Protocols in Current Issue
    Intestinal Enteroid Culture for Human Astroviruses
    Authors:  Irene A. Owusu, Carmen Mirabelli and Abimbola O. Kolawole, date: 07/20/2020, view: 2322, Q&A: 0
    [Abstract] Human astroviruses (HAstV) are non-enveloped, positive-sense single stranded RNA viruses that typically cause gastroenteritis in children, the elderly and among immunocompromised individuals. Some HAstV species have also been implicated in neurological diseases. It is important to study these viruses to understand the pathogenesis and develop ...
    Human Astrovirus Propagation, Purification and Quantification
    Authors:  Shauna Marvin, Victoria Meliopoulos and Stacey Schultz-Cherry, date: 03/20/2014, view: 10285, Q&A: 0
    [Abstract] Astrovirus are small, nonenveloped, single-stranded RNA viruses that cause diarrhea in a wide variety of mammals and birds. Here, we describe astrovirus propagation, purification and titration. The Caco-2 human intestinal adenocarcinoma cell line is most widely used for studying astrovirus, although other cell lines, such as 293, T84 and LLC-MK2 ...

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