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    Protocols in Current Issue
    Detection of piggyBac-mediated Transposition by Splinkerette PCR in Transgenic Lines of Strongyloides ratti
    Authors:  Hongguang Shao and James B. Lok, date: 01/05/2014, view: 13435, Q&A: 0
    [Abstract] Splinkerette PCR (spPCR) is a newly developed and efficient method to ascertain and characterize genomic insertion sites of transgenes. The method described in this protocol was successfully applied to confirm piggyBac transposon-mediated integration of transgenes into chromosomes of the parasitic nematode Strongyloides ratti. ...



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