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Detection of
piggyBac
-mediated Transposition by Splinkerette PCR in Transgenic Lines of
Strongyloides ratti
Authors:
Hongguang Shao
and
James B. Lok
,
date:
01/05/2014,
view:
13435,
Q&A:
0
[Abstract] Splinkerette PCR (spPCR) is a newly developed and efficient method to ascertain and characterize genomic insertion sites of transgenes. The method described in this protocol was successfully applied to confirm
piggyBac
transposon-mediated integration of transgenes into chromosomes of the parasitic nematode
Strongyloides ratti
. ...
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