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Measurements of Root Colonized Bacteria Species

Authors: Juan Ignacio Vílchez, Yu Yang, Dian Yi and Huiming Zhang, date: 04/05/2021, view: 3146, Q&A: 0

[Abstract]

Root-associated bacteria are able to influence plant fitness and vigor. A key step in understanding the belowground plant-bacteria interactions is to quantify root colonization by the bacteria of interest. Probably, genetic engineering with fluorescence markers is the most powerful way to monitor bacterial strains in plant. However, this could

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Endophytic Microbial Community DNA Extraction from the Plant Phyllosphere

Authors: Carlos A. Ruiz-Pérez and María Mercedes Zambrano, date: 02/20/2017, view: 9554, Q&A: 1

[Abstract] The plant phyllosphere, which represents all plant parts that are above the ground, is considered one of the most extensive ecosystems to be colonized by microorganisms, both at the surface as epiphytes or as endophytes within the plant. These plant-associated microbial communities are reservoirs of microbial diversity and they can be important ...

Extraction and Measurement of Strigolactones in Sorghum Roots

Authors: Kaori Yoneyama, Xiaonan Xie, Takahito Nomura and Koichi Yoneyama, date: 03/20/2016, view: 9327, Q&A: 0

[Abstract] Strigolactones (SLs) are carotenoid-derived signaling chemicals containing two lactone moieties in their structures and induce seed germination of root parasitic plants, Striga and Orobanche spp. In the rhizosphere, SLs are essential host recognition signals not only for root parasitic plants but also for arbuscular mycorrhizal ...

Quantification of the Volume and Surface Area of Symbiosomes and Vacuoles of Infected Cells in Root Nodules of Medicago truncatula

Authors: Aleksandr Gavrin and Elena E. Fedorova, date: 12/05/2015, view: 9620, Q&A: 0

[Abstract] Legumes are able to form endosymbiotic interactions with nitrogen-fixing rhizobia. Endosymbiosis takes shape in formation of a symbiotic organ, the root nodule. Medicago truncatula (M. truncatula) nodules contain several zones representing subsequent stages of development. The apical part of the nodule consists of the meristem ...

Establishment of a Symbiotic in vitro System between a Green Meadow Orchid and a Rhizoctonia-like Fungus

Authors: Enrico Ercole, Michele Rodda, Mariangela Girlanda and Silvia Perotto, date: 05/20/2015, view: 10689, Q&A: 0

[Abstract] Symbiotic orchid seed germination in an in vitro system allows the growth of mycorrhizal protocorms and plantlets for scientific purposes. Orchids in nature need to establish a mycorrhizal symbiosis with fungal partners to germinate and develop into adult plants. Here we present a protocol for symbiotic germination of the terrestrial ...

Quantification of Flavin Production by Bacteria

Authors: Svetlana N. Yurgel, Joseph Lynch, Jennifer Rice, Neil Adhikari and Sanja Roje, date: 08/05/2014, view: 9179, Q&A: 0

[Abstract] This protocol provides a simple and fast method of quantification for intracellular flavin content, and for flavin secretion by bacteria. Intracellular flavins are extracted from bacterial pellets, and secreted flavins are examined in the cell growth medium. Flavins are separated and measured using HPLC with fluorescence detection, and quantified ...

RNA Isolation From Meloidogyne Spp. Galls

Authors: Alejandra García, Marta Barcala, Javier Cabrera, Carmen Fenoll and Carolina Escobar, date: 09/05/2013, view: 8916, Q&A: 0

[Abstract] We describe an efficient method to obtain a sufficient quantity of RNA from nematode-induced galls with a high quality and integrity, proved to be appropriate for transcriptomic analysis, i.e. real time PCR, microarray hybridization or second generation sequencing. This protocol is efficient for small quantities of galls (organs with high ...

Indole Derivative Feeding Test and Detection of TRP and Indole derivatives by Thin Layer Chromatography

Authors: Magdalena Hilbert, Lars M. Voll, Jörg Hofmann and Alga Zuccaro, date: 06/20/2013, view: 9963, Q&A: 0

[Abstract] The mutualistic root endophyte Piriformospora indica colonizes a wide range of plants and the colonization of root cells by this fungus is very often associated with beneficial effects to its host, such as growth promotion and increased biotic and abiotic stress tolerance. These traits could be based on general mechanisms and signaling ...

Growth Assay and Detection of TRP and Indole Derivatives in Piriformospora indica Culture Supernatant by LC-MS/MS

Authors: Magdalena Hilbert, Lars M. Voll, Jörg Hofmann and Alga Zuccaro, date: 06/20/2013, view: 11734, Q&A: 0

[Abstract] The mutualistic root endophyte Piriformospora indica colonizes a wide range of plants and the colonization of root cells by this fungus is very often associated with beneficial effects to its host, such as growth promotion and increased biotic and abiotic stress tolerance. These traits may be based on general mechanisms and signaling ...

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