Molecular Biology


    Protocols in Current Issue
    Conjugation of Duplexed siRNN Oligonucleotides with DD-HyNic Peptides for Cellular Delivery of RNAi Triggers
    Authors:  Alexander S. Hamil, Khirud Gogoi and Steven F. Dowdy, date: 04/05/2016, view: 7606, Q&A: 0
    [Abstract] Despite the great promise that short interfering RNA (siRNA) induced RNAi responses hold as a therapeutic modality, due to their size (~15 kDa) and high negative charge (Bumcrot et al., 2006), siRNAs have no bioavailability and require a delivery agent to enter cells (Figure 1). TAT peptide transduction domain (PTD) has been developed as ...
    Post-crystallization Improvement of RNA Crystals by Synergistic Ion Exchange and Dehydration
    Authors:  Jinwei Zhang and Adrian R. Ferré-D’Amaré, date: 09/05/2015, view: 7516, Q&A: 0
    [Abstract] Compared to the recent dramatic growth in the numbers of genome-wide and functional studies of complex non-coding RNAs, mechanistic and structural analyses have lagged behind. A major technical bottleneck in the structural determination of large RNAs and their complexes is preparation of diffracting crystals. Empirically, a vast majority of such ...
    Synthesis of 5’ end-labeled RNA
    Author:  Harald Putzer, date: 07/20/2012, view: 12554, Q&A: 2
    [Abstract] 5’ end-labeled RNA molecules are useful substrates to analyse the endo- and exonucleolytic activities of various ribonucleases. Here two protocols are given to synthesize P32 labeled RNAs with a 5’ PPP or 5’ P moiety. 5’ exoribonucleases generally do not work on 5’ PPP RNA and require a 5’ P substrate. The activity of certain ...

    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.