Cancer Biology


    Protocols in Current Issue
    Image-based Quantification of Macropinocytosis Using Dextran Uptake into Cultured Cells
    Authors:  Anh H. Le and Laura M. Machesky, date: 04/05/2022, view: 1053, Q&A: 0

    Macropinocytosis is an evolutionarily conserved process, which is characterized by the formation of membrane ruffles and the uptake of extracellular fluid. We recently demonstrated a role for CYFIP-related Rac1 Interactor (CYRI) proteins in macropinocytosis. High-molecular weight dextran (70kDa or higher) has generally been used as a marker for

    Spherical Invasion Assay: A Novel Method to Measure Invasion of Cancer Cells
    [Abstract] The invasion of tumor cells into the neighboring blood vessels and lymph nodes is a vital step for distant metastasis. Traditionally, the invasive activity of growth factors (or the anti-invasive activity of drugs) is measured with the Boyden chamber assay. However, this assay has a few disadvantages like poor physiological relevance of transwell ...
    Studying Chemotactic Migration in Dunn Chamber: An Example Applied to Adherent Cancer Cells
    Authors:  Khedidja Benseddik and Kossay Zaoui, date: 02/05/2022, view: 1282, Q&A: 0

    Cell migration is a vital process in the development of multicellular organisms. When deregulated, it is involved in many diseases such as inflammation and cancer metastisation. Some cancer cells could be stimulated using chemoattractant molecules, such as growth factor Heregulin β1. They respond to the attractant or repellent gradients

    Preparation and Cultivation of Colonic and Small Intestinal Murine Organoids Including Analysis of Gene Expression and Organoid Viability
    Authors:  Luisa Klemke, Joshua P. Blume, Tiago De Oliveira and Ramona Schulz-Heddergott, date: 01/20/2022, view: 2584, Q&A: 0

    Organoids are complex three-dimensional structures, which contain different cell types and help to overcome many limitations of conventional 2D cell culture techniques. Here, we present a protocol for the cultivation of murine matched-pairs of small intestinal and colonic epithelial organoids, and colonic tumor organoids derived from the chemical

    Measurement of Bone Metastatic Tumor Growth by a Tibial Tumorigenesis Assay
    Authors:  Baotong Zhang, Xin Li, Wei-Ping Qian, Daqing Wu and Jin-Tang Dong, date: 11/20/2021, view: 2057, Q&A: 0

    Bone metastasis is a frequent and lethal complication of many cancer types (i.e., prostate cancer, breast cancer, and multiple myeloma), and a cure for bone metastasis remains elusive. To recapitulate the process of bone metastasis and understand how cancer cells metastasize to bone, intracardiac injection and intracaudal arterial animal models

    Imaging of Human Cancer Cells in 3D Collagen Matrices
    Authors:  Karin Pfisterer, Brooke Lumicisi and Maddy Parsons, date: 01/20/2021, view: 3908, Q&A: 0

    Research on cell migration and interactions with the extracellular matrix (ECM) was mostly focused on 2D surfaces in the past. Many recent studies have highlighted differences in migratory behaviour of cells on 2D surfaces compared to complex cell migration modes in 3D environments. When embedded in 3D matrices, cells constantly sense the

    Single Cell Migration Assay Using Human Breast Cancer MDA-MB-231 Cell Line
    Authors:  David M. Gau and Partha Roy, date: 04/20/2020, view: 4929, Q&A: 0
    [Abstract] Cell migration is a fundamental cellular process that plays a crucial role in many physioglogical and pathological processes such as wound healing or cancer metastasis. Many assays have been developed to examine cell migration, such as the wound healing or scratch assay, Boyden Chamber or transwell assay, and the method we will describe here, ...
    In vivo Tumor Growth and Spontaneous Metastasis Assays Using A549 Lung Cancer Cells
    [Abstract] Metastasis accounts for the majority of cancer related deaths. The genetically engineered mouse (GEM) models and cell line-based subcutaneous and orthotopic mouse xenografts have been developed to study the metastatic process. By using lung cancer cell line A549 as an example, we present a modified protocol to establish the cell line-based ...
    Bone-in-culture Array to Model Bone Metastasis in ex vivo Condition
    Authors:  Hai Wang and Xiang H.-F. Zhang, date: 01/20/2020, view: 3145, Q&A: 0
    [Abstract] Bone is the most frequently affected organ by metastases of breast cancer and prostate cancer. Our knowledge on bone metastasis is extremely limited due to the lack of potent and efficient experimental models. We developed the “Bone-In-Culture Array (BICA)” platform to model the bone colonization of cancer cells in ex vivo cultures. The ...
    Analysis of Random Migration of Cancer Cells in 3D
    Authors:  Sai P. Visweshwaran and Alexis Gautreau, date: 01/05/2020, view: 4552, Q&A: 1
    [Abstract] The ability of cancer cells to migrate through a complex three-dimensional (3D) environment is a hallmark event of cancer metastasis. Therefore, an in vitro migration assay to evaluate cancer cell migration in a 3D setting is valuable to examine cancer progression. Here, we describe such a simple migration assay in a 3D ...

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