Microbiology

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    Protocols in Current Issue
    Purification of the Bacterial Amyloid “Curli” from Salmonella enterica Serovar Typhimurium and Detection of Curli from Infected Host Tissues
    [Abstract]

    Microbiologists are learning to appreciate the importance of “functional amyloids” that are produced by numerous bacterial species and have impacts beyond the microbial world. These structures are used by bacteria to link together, presumably to increase survival, protect against harsh conditions, and perhaps to influence cell-cell

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    Listeria innocua Biofilm Assay Using NanoLuc Luciferase
    Authors:  Aleš Berlec, Nika Janež, Meta Sterniša, Anja Klančnik and Jerica Sabotič, date: 02/05/2022, view: 1147, Q&A: 0
    [Abstract]

    Biofilms serve as a bacterial survival strategy, allowing bacteria to persist under adverse environmental conditions. The non-pathogenic Listeria innocua is used as a surrogate organism for the foodborne pathogen Listeria monocytogenes, because they share genetic and physiological similarities and can be used in a Biosafety Level 1 laboratory.

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    An Inexpensive Imaging Platform to Record and Quantitate Bacterial Swarming
    Authors:  Weijie Chen, Sridhar Mani and Jay X. Tang, date: 09/20/2021, view: 1456, Q&A: 0
    [Abstract]

    Bacterial swarming refers to a rapid spread, with coordinated motion, of flagellated bacteria on a semi-solid surface (Harshey, 2003). There has been extensive study on this particular mode of motility because of its interesting biological and physical relevance, e.g., enhanced antibiotic resistance (Kearns, 2010) and turbulent collective motion

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    Development and Quantitation of Pseudomonas aeruginosa Biofilms after in vitro Cultivation in Flow-reactors
    Authors:  Yingdan Zhang, Jingru Zhao, Hang Cheng, Jing Wang, Liang Yang and Haihua Liang, date: 08/20/2021, view: 1314, Q&A: 0
    [Abstract]

    Characterization of biofilm formation and metabolic activities is critical to investigating biofilm interactions with environmental factors and illustrating biofilm regulatory mechanisms. An appropriate in vitro model that mimics biofilm in vivo habitats therefore demands accurate quantitation and investigation of biofilm-associated activities.

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    Inkjet 3D Printing of Polymers Resistant to Fungal Attachment
    Authors:  Yinfeng He, Cindy Vallieres, Morgan R. Alexander, Ricky D. Wildman and Simon V. Avery, date: 05/05/2021, view: 2944, Q&A: 0
    [Abstract]

    Inkjet 3D printing is an additive manufacturing method that allows the user to produce a small batch of customized devices for comparative study versus commercial products. Here, we describe the use of a commercial 2D ink development system (Dimatix material printing) to manufacture small batches of 3D medical or other devices using a recently

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    Candida Biofilm Formation Assay on Essential Oil Coated Silicone Rubber
    [Abstract]

    Development of biofilm associated candidemia for patients with implanted biomaterials causes an urgency to develop antimicrobial and biofilm inhibitive coatings in the management of recalcitrant Candida infections. Recently, there is an increase in the number of patients with biofilm formation and resistance to antifungal therapy. Therefore, there

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    Analysis of Pseudomonas aeruginosa c-di-GMP High and Low Subpopulations Using Flow-assisted Cell Sorting (FACS) and Quantitative Reverse Transcriptase PCR (qRT-PCR)
    Authors:  Catherine R. Armbruster and Matthew R. Parsek, date: 01/20/2021, view: 2314, Q&A: 0
    [Abstract]

    Cyclic diguanylate monophosphate (c-di-GMP) is a second messenger signaling molecule that drives the transition from planktonic to the biofilm mode of growth in many bacterial species. Pseudomonas aeruginosa has at least two surface sensing systems that produce c-di-GMP in response to surface attachment, the Wsp and Pil-Chp systems. We recently

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    Bacterial Adhesion Kinetics in a High Throughput Setting in Seconds-minutes Time Resolution
    Authors:  Nimrod Shteindel and Yoram Gerchman, date: 01/20/2021, view: 1663, Q&A: 0
    [Abstract] Bacterial surface adhesion, the first step in many important processes including biofilm formation and tissue invasion, is a fast process that occurs on a time scale of seconds. Adhesion patterns tend to be stochastic and spatially heterogeneous, especially when bacteria are present in low population densities and at early stages of adhesion to ...
    Simple Time-lapse Imaging for Quantifying the Hydrostatic Production of Oxygenic Photogranules
    Authors:  Esmee D. Joosten, Jérôme Hamelin and Kim Milferstedt, date: 10/05/2020, view: 1997, Q&A: 0
    [Abstract] Oxygenic photogranules (OPGs) are dense, three-dimensional aggregates containing a syntrophic, light-driven microbial community. Their temporal and spatial development interests microbial ecologists working at the bioprocess engineering interface, as this knowledge can be used to optimize biotechnological applications, such as wastewater treatment ...
    Evaluation of Viable Cells in Pseudomonas aeruginosa Biofilms by Colony Count and Live/Dead Staining
    Authors:  Magdalena Pezzoni, Ramón Augusto Pizarro and Cristina Susana Costa, date: 09/20/2020, view: 3248, Q&A: 0
    [Abstract] Pseudomonas aeruginosa is a human pathogen capable to form robust biofilms. P. aeruginosa biofilms represent a serious problem because of the adverse effects on human health and industry, from sanitary and economic points of view. Typical strategies to break down biofilms have been long used, such as the use of disinfectants or ...



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