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E. coli Genomic DNA Extraction
Published: Jul 20, 2011 DOI: 10.21769/BioProtoc.97 Views: 217215
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Abstract
This protocol uses phenol/chloroform method to purify genomic DNA without using commercial kits.
Background
Materials and Reagents
- Tris base (Calbiochem-Behring)
- Proteinase K (Sigma-Aldrich)
- Phenol\chloroform (1: 1) (EM Science)
- 200 proof ethanol (Pharmco-AAPER)
- RNAase (Life Technologies, Invitrogen™)
- Ethanol
- SDS
- EDTA
- Tryptone
- Yeast extract
- NaCl
- LB medium (see Recipes)
- TE buffer (see Recipes)
- Lysis buffer (see Recipes)
Equipment
- Tabletop centrifuge (Eppendorf)
- 1.5 ml Eppendorf tube
- Incubator
- Gloves
Procedure
Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.
Category
Microbiology > Microbial genetics > DNA
Molecular Biology > DNA > DNA extraction
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