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Hi, thanks for the protocol. Does this protocol yield high molecular...

Anthony Bayega Apr 4, 2016

Protocol: E. coli Genomic DNA Extraction

Hi, thanks for the protocol. Does this protocol yield high molecular weight DNA? I am interested in extracting like 80kb and above. Also, after the lysis step, is the lysate not too thick (mucous) to handle and even seperate the proteins out after phenol-chloroform?

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Anthony Bayega Answered Apr 6, 2016

McGill University

I am running the protocol now and will let u know the results. Thanks

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Fanglian He Author Answered Apr 5, 2016

Bio-protocol

Hi Anthony,

Yes, you should expect to see DNA with high MW by using this protocol. And, the lysate was not too thick to handle in my hands.

Good luck!
Fanglian

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