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dear Fanglian, I plan on taking a study on the detection of...

samaila waziri May 11, 2016

Protocol: E. coli Genomic DNA Extraction

dear Fanglian, I plan on taking a study on the detection of E. coli in groundwater samples. I need a E. coli DNA extraction and amplification procedure that does not require the use of Kit. you could post the methodology/procedure to my e-mail address: smlbnjmn@gmail.com

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samaila waziri Answered May 21, 2016

Ahmadu Bello University, Zaria, Kaduna State, Nigeria

thank you fanglian

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Fanglian He Author Answered May 20, 2016

Bio-protocol

There are a number of companies (i.e. Qiagen) providing the related commercial products with detailed manufacture's instructions. Thus, Bio-protocol does not publish this type of protocols as a separate publication. You could either find the instructions on the company websites or contact them directly.

Good luck!
Fanglian

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samaila waziri Answered May 18, 2016

Ahmadu Bello University, Zaria, Kaduna State, Nigeria

dear fanglian, I will also require a detailed procedure for the detection of E coli in water samples that require a DNA extraction Kit and PCR mastermix

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Fanglian He Author Answered May 11, 2016

Bio-protocol

Hi Samaila,

Please feel free to save a copy of the above protocol at your end.

Good luck,
Fanglian

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