I am preparing to analyze fish larval body protein by Bradford method. Recently I found NaOH is good to prepare protein solution by the sample. But I still have few questions..
1.Is it necessary to add 100 ul water to dye regent when use the blank. If the dye regent is used without 100ul of H2O in blank, is it a issue? Because I already diluted my bio-rad dye regent four times with de-ionized water as per instruction?
2. In protein quantification by UV-1800, do we need use cubic curve instead of linear calibration curve?
3. In the calibration curve, do we need to select the the intersect as zero or not?
4. Actually to prepare 2 ml of protein solution (100 ul will be pipette out as the sample to add with 5 ml dye regent later), how much mg of dry sample or wet sample do we need to take ?
3/15/2020 10:10:11 AM Reply