Hi, may I ask for which solutions the Bradford assay can be...
Hi,
may I ask for which solutions the Bradford assay can be used, is it suitable for to measure a protein conc. from organic cream?
2
answers
Sort by: most helpful / most recent
It also depends on what your "organic cream" is. If it contains a lot of non-protein material, (i.e
Lipids) you may need to extract protein content before assaying using something like trichloroacetic acid to precipitate proteins.
Mike
A Bradford's assay is the most common assay for protein concentration. If protein concentration for your sample is completely unknown, you may need to dilute it sufficiently to bring it into the range of your prepared standards. Avoid using SDS or ionic detergents in sample preparation as they may interfere with results.
Mike
References (optional)
Add more
Post an Answer