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hi every one , i am facing problem is plotting std protein...

Sneha Shriram More Oct 20, 2017

hi every one , i am facing problem is plotting std protein graph. here i used 1mg/ml std protein. different concentration used for plotting is 0.2mg / 4ml 0. 4mg/ 4ml 0.6mg/ 4ml 0.8 mg/ 4ml and 1mg /4ml. and for estimation of protein only 50 ul sample was used form each Read more more

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MICHAEL D SMALLING Answered Dec 22, 2018

Louisiana Tech University

Hi Sneha, be advised that you are using very low concentrations for your Bradford's assay standards. Typically, you would use those amounts per 1mL rather than 4mL. However, as you mix your unknown samples at the same rate as your standards, 50uL/2.5mL, the readings for your standard will correlate to the same protein concentration in your unknown samples.

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