Plants

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    Agrobacterium-mediated Transformation of Nicotiana tabacum cv. Xanthi Leaf Explants
    Authors:  Ana Lilia Rosales-Campos and Abel Gutiérrez-Ortega, date: 01/20/2019, view: 29, Q&A: 0
    Plant transformation has become an important technology for the large-scale production of a number of goods, ranging from vaccines and pharmaceutical products to biofuel. A plant that is frequently used for this purpose is Nicotiana tabacum ...
    A Simplified Hydroponic Culture of Arabidopsis
    Authors:  Houqing Zeng, Chao Xia, Cankui Zhang and Li-Qing Chen, date: 12/20/2018, view: 164, Q&A: 0
    Hydroponic culture systems are widely used in research due to their intrinsic properties, such as easily altering nutrient composition, applying chemical treatments like metal, salt and hormones in the growth media, and collecting the root sample. ...
    Screening and Genetic Analysis of Ethylene-Response Mutants in Etiolated Rice Seedlings
    Authors:  Cui-Cui Yin, Biao Ma, He Zhao, Shou-Yi Chen and Jin-Song Zhang, date: 09/05/2018, view: 490, Q&A: 0
    Ethylene, the simplest gaseous plant phytohormone, is involved in the control of rice growth and development processes, but the mechanism of ethylene regulating these pathways remains unclear in rice. Recent studies have found that ...
    Growth of Chlamydomonas reinhardtii under Circadian Conditions
    Authors:  Volker Wagner and Maria Mittag, date: 08/20/2018, view: 815, Q&A: 0
    The green biflagellate unicellular alga Chlamydomonas reinhardtii serves as a model to study fundamental biological processes such as the structure and function of flagella or light-driven processes including photosynthesis, its behavioral ...
    Microscopic Observation of Subcellular GFP-tagged Protein Localization in Rice Anthers
    Authors:  Seijiro Ono and Ken-Ichi Nonomura, date: 08/05/2018, view: 624, Q&A: 0
    This protocol demonstrates a simple method to determine the subcellular localization of fluorescence-tagged proteins on the vibratome sections of rice developing anthers. If a cell type-specific promoter is used to drive the tagged protein-encoding ...
    Quantification of Root Colonizing Bacteria
    Authors:  Maged M. Saad, Axel de Zélicourt, Eleonora Rolli , Lukas Synek and Heribert Hirt , date: 07/20/2018, view: 677, Q&A: 0
    Here we describe a simple method to quantify the number of viable bacteria (e.g., Enterobacter sp. SA187) that colonize the root system of Arabidopsis thaliana.
    Protocol to Test the Effect of Sorbitol in vitro on Hybrid Larch (Larix x eurolepis Henry) Emblings
    Authors:  Bettina Johst and María del Carmen Dacasa Rüdinger, date: 07/20/2018, view: 678, Q&A: 0
    This protocol presents a method to test the effect of sorbitol in vitro on hybrid larch plants derived from somatic embryogenesis. We have tested four different media variants, one control variant without sorbitol and three variants of ...
    Visualization of Growth and Morphology of Fungal Hyphae in planta Using WGA-AF488 and Propidium Iodide Co-staining
    Authors:  Amey Redkar, Elaine Jaeger and Gunther Doehlemann, date: 07/20/2018, view: 950, Q&A: 0
    Fungal pathogens colonizing plants show a varying degree of symptoms. Microscopy techniques have been used to study the infection and proliferation of fungal hyphae inside the host. One of the best optimized and commonly used method is the ...
    RNA Immunoprecipitation (RIP) Sequencing of Pri-miRNAs Associated with the Dicing Complex in Arabidopsis
    Authors:  Ziwei Li, Songxiao Zhong, Wenye Wu and Binglian Zheng, date: 07/05/2018, view: 1006, Q&A: 0
    RNA immunoprecipitation (RIP) is an antibody-based technique used to map in vivo RNA-protein interactions. DBR1, an RNA debranching enzyme, is responsible for the debranching of lariat RNA, for the degradation and turnover of lariat RNAs. ...
    Micropropagation of Prickly Pear by Axillary Shoot Proliferation
    A protocol for the axillary bud proliferation of prickly pear (Opuntia; Cactaceae) is presented. This genus is widely used as a crop in the arid and semi-arid areas of the globe worldwide, providing numerous benefits for human and animal ...