Plants

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Western Blot in Maize

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    Transient Transformation of Maize BMS Suspension Cells via Particle Bombardment
    Authors:  Liang-Zi Zhou and Thomas Dresselhaus, date: 12/05/2019, view: 327, Q&A: 0
    Maize is one of the most important crop species and serves also as a model plant for grass research. A major bottleneck in maize research is stable transformation, which is both time and cost consuming, but also a technical challenge for most labs ...
    A Simple Protocol for Informative Visualization of Enriched Gene Ontology Terms
    Authors:  Titouan Bonnot, Morgane B. Gillard and Dawn H. Nagel, date: 11/20/2019, view: 915, Q&A: 1
    In genome-scale datasets, Gene Ontology (GO) enrichment is a common analysis to highlight functions over-represented or under-represented in a subset of differentially expressed genes to elucidate the biological significance of the results. However, ...
    Isolation and Efficient Maize Protoplast Transformation
    Authors:  Lina Gomez-Cano, Fan Yang and Erich Grotewold, date: 08/20/2019, view: 1044, Q&A: 0
    Establishing plant gene function and the study of gene expression regulation often require that genes (or gene variants) are introduced into plants and their activity somehow assayed. Some plants (e.g., Arabidopsis) are easily ...
    Quantifying MAMP-induced Production of Reactive Oxygen Species in Sorghum and Maize
    Plants have the ability to recognize microbe-associated molecular patterns (MAMPs) and mount a defense response. The level of the MAMP response can vary depending on genetic and environmental factors. The most commonly studied MAMPs are flg22, a ...
    Fluorescence Immunolocalization in Mitotic Chromosome Spreads from Maize Embryos
    Authors:  Jing-Han Liu, Ya-zhong Wang, Lin Chen and Yan He, date: 06/20/2019, view: 813, Q&A: 0
    Immunolocalization is a necessary technique in the study of chromosome structure and function. It can provide important information for better understanding the relation between epigenetic modifications and chromosome condensation. In this protocol, ...
    Double Labeling of Microtubules and Actin Filaments in Maize Leaf Division Zone
    Authors:  Qiong Nan, Janette Mendoza and Michelle Renee Facette, date: 06/20/2019, view: 991, Q&A: 0
    Maize leaves grow in a gradient. The base of young maize leaves contains a high proportion of symmetrically and asymmetrically dividing cells, making the leaf an effective model system for the study of cell division. Here, we describe a double ...
    Heat Induced Epitope Retrieval (HIER) Assisted Protein Immunostaining in Maize
    Authors:  Katsutoshi Tsuda and George Chuck, date: 06/05/2019, view: 750, Q&A: 0
    Protein immunostaining provides important spatio-temporal information about gene expression. Even using high quality antibodies, signal reproducibility and specificity can be problematic depending on tissue fixation methods. For example, ...
    Western Blot in Maize
    Author:  María Jazmín Abraham-Juárez, date: 06/05/2019, view: 1914, Q&A: 0
    Since its introduction in 1979 (Towbin et al., 1979), protein blotting (Western blot) has become a routine tool in molecular biology research. It is used to detect low amounts of proteins in complex samples or to monitor protein expression ...
    Protein Immunoprecipitation in Maize
    Author:  María Jazmín Abraham-Juárez, date: 06/05/2019, view: 890, Q&A: 0
    Protein immunoprecipitation (IP) is a method used to identify cellular protein complexes. Whence, antibodies that specifically recognize a native bait protein or an epitope tag fused to the protein of interest allow the protein of interest to be ...
    Maize Protein Extraction for Different Downstream Applications
    Author:  María Jazmín Abraham-Juárez, date: 05/20/2019, view: 1059, Q&A: 0
    Sample preparation is a key step for making plant proteomics analyses reproducible. The large amount of organic compounds, complex polysaccharides, proteases and other cellular components in vascular plants interfere with the analysis of proteins ...