Search

Prokaryotes

Categories

    A Quick and Easy Method for Making Competent Escherichia coli Cells for Transformation Using Rubidium Chloride
    Authors:  Nidhi Sharma, M. Ximena Anleu Gil and Diego Wengier, date: 11/05/2017, view: 790, Q&A: 0
    This protocol describes a quick and efficient method to make competent E. coli cells for transformation using rubidium chloride. Commercial competent cells are expensive and this protocol provides a cheaper alternative to them.
    Purifying Properly Folded Cysteine-rich, Zinc Finger Containing Recombinant Proteins for Structural Drug Targeting Studies: the CH1 Domain of p300 as a Case Example
    Authors:  Yong Joon Kim, Stefan Kaluz, Anil Mehta, Emily Weinert, Shannon Rivera and Erwin G. Van Meir, date: 09/05/2017, view: 1304, Q&A: 1
    The transcription factor Hypoxia-Inducible Factor (HIF) complexes with the coactivator p300, activating the hypoxia response pathway and allowing tumors to grow. The CH1 and CAD domains of each respective protein form the interface between p300 and ...
    A General Protocol for GST Pull-down
    Author:  Lili Jing, date: 01/20/2012, view: 48907, Q&A: 3
    GST-Pull down assay is an effective way to examine the direct binding of two proteins in vitro. This protocol is based on GST pull down system from GE healthcare, and uses the binding of unplugged/MuSK receptor and Wnt ligand as an example ...
    The Inoue Method for Preparation and Transformation of Competent E. coli: "Ultra Competent" Cells
    Author:  Hogune Im, date: 10/20/2011, view: 13314, Q&A: 1
    This protocol differs from other procedures in that the bacterial culture is grown at 18 °C rather than the conventional 37 °C. Otherwise, the protocol is unremarkable and follows a fairly standard course. Why growing the cells at low temperature ...
    Expression and Purification of GST-tagged Proteins from E. coli
    Author:  Lin Fang, date: 09/20/2011, view: 12882, Q&A: 1
    This protocol describes a method for the small and large-scale expression and purification of GST proteins. Due to the diverse nature of proteins, a small-scale expression and purification test is always recommended.
    E. coli Genomic DNA Extraction
    Author:  Fanglian He, date: 07/20/2011, view: 106743, Q&A: 48
    This protocol uses phenol/chloroform method to purify genomic DNA without using commercial kits.
    Laemmli-SDS-PAGE
    Author:  Fanglian He, date: 06/05/2011, view: 81207, Q&A: 9
    Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is used to separate proteins with relative molecular mass no smaller than 10 KD. Very small proteins (<10 KD) are difficult to resolve due to low ability of binding to SDS, ...
    Labeling Protein with Thiol-reactive Probes
    Author:  Qingrong Yan, date: 06/05/2011, view: 6604, Q&A: 1
    This protocol is used to label protein or peptide with a maleinmide or iodoacetamide conjugated fluorescent probe through the free cysteine.
    RbCl Super Competent Cells
    Author:  Xiyan Li, date: 06/05/2011, view: 13850, Q&A: 2
    This method is used to inexpensively prepare home-made competent cells of E. coli. The transformation efficiency is at the high end of chemical-efficient competent cells, and close to library-efficient competent cells.