Plant Science

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    Barley Transformation by Particle Bombardment Using Callus
    Author:  Sung-Yong Kim, date: 06/05/2020, view: 1648, Q&A: 0
    The delivery of desired construct into plant cells relies mainly on Agrobacterium-mediated transformation. However, Agrobacterium transformation is still very challenging for barley as only one genotype is amendable to ...
    In vitro Protein-DNA Binding Assay (AlphaScreen® Technology)
    Authors:  Mika Nomoto, Yasuomi Tada and Hironaka Tsukagoshi, date: 02/05/2019, view: 5397, Q&A: 0
    Identification of specific DNA binding sites of transcription factors is important in understanding their functions. Recent techniques allow us to investigate genome-wide in vivo binding positions by chromatin immunoprecipitation combined ...
    GUS Staining of Guard Cells to Identify Localised Guard Cell Gene Expression
    Authors:  Zhao Liu, Wei Wang, Chun-Guang Zhang, Jun-Feng Zhao and Yu-Ling Chen , date: 07/20/2017, view: 14571, Q&A: 0
    Determination of a gene expression in guard cells is essential for studying stomatal movements. GUS staining is one means of detecting the localization of a gene expression in guard cells. If a gene is specially expressed in guard cells, the whole ...
    3’ Rapid Amplification of cDNA Ends (3’ RACE) Using Arabidopsis Samples
    Production of functional eukaryotic RNA is a very elaborate process that involves a complex interplay between transcription and various RNA processing activities, including splicing, 5’ capping, and 3’ cleavage and polyadenylation (Bentley, 2014). ...
    5’ Rapid Amplification of cDNA Ends (5’ RACE) of Agrobacterial T-DNA Genes within Transformed Plant Sample
    Author:  Yi Zhang, date: 09/20/2015, view: 10336, Q&A: 0
    The T-DNA (transferred-DNA) region of virulent Agrobacterium tumefaciens (A. tumefaciens) strain is transferred and integrated into the plant genome, and thereby the T-DNA genes are expressed in transformed plant cells. This ...
    SGR-based Reporter to Assay Plant Transcription Factor-promoter Interactions
    Authors:  Shisong Ma and Savithramma Dinesh-Kumar, date: 08/20/2014, view: 9017, Q&A: 0
    We developed an in vivo method to assay plant transcription factor (TF)–promoter interactions using the transient expression system in Nicotiana benthamiana (N. benthamiana) plants. The system uses the Arabidopsis stay ...
    Determination of Luciferase Activity in Arabidopsis seedling
    Authors:  Mohan TC, Gabriel Castrillo and Antonio Leyva, date: 06/20/2014, view: 10789, Q&A: 0
    There are different direct and indirect methods available to study gene expression in plant systems. In this protocol we describe a modified expression assay using transgenic plants expressing the luciferase gene under the control of phosphate ...
    Modified Single-Cell Transient Gene Expression Assay in Barley Epidermal Cells
    Authors:  Shiwei Bai, Cheng Chang, Xinyun Han and Qian-Hua Shen, date: 05/05/2013, view: 8563, Q&A: 0
    Transient gene expression via biolistic particle delivery is a widely used technique for gene functional analysis in plants. In this protocol we describe a modified single-cell transient expression assay through transformation with a particle inflow ...



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