Molecular Biology

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Western Blot in Maize

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    Isolation of PCR-quality Genomic DNA from Soils Impacted with Extra Heavy Crude Oil
    For the study of microbial communities in samples of soils impacted with extra heavy crude oil, it is necessary to perform molecular analyses. Due to the difficulty of oil matrix handling, there are very few protocols reported in writing. Also, one ...
    Plasmid Extract from Budding Yeast (Saccharomyces cerevisiae)
    Author:  Gal Haimovich, date: 07/20/2018, view: 3538, Q&A: 0
    Plasmids are widely used tools in yeast research. In many cases, plasmid libraries are used in genetic screens or in yeast two hybrid screens. In such cases, it is necessary to extract plasmids carrying unknown genetic elements from positive clones ...
    Root Gall Formation, Resting Spore Isolation and High Molecular Weight DNA Extraction of Plasmodiophora brassicae
    Authors:  Sara Mehrabi, Suzana Stjelja and Christina Dixelius, date: 06/05/2018, view: 2609, Q&A: 0
    Isolation of DNA from obligate biotrophic soil-borne plant pathogens is challenging. This is because of their strict requirement of living plant tissue for their growth and propagation. A soil habitat further imposes risk of contamination from other ...
    Isolation of Genomic DNA from Chlamydomonas reinhardtii
    Author:  Tyler M Wittkopp, date: 05/05/2018, view: 4882, Q&A: 0
    Chlamydomonas reinhardtii is a soil-dwelling eukaryotic green alga that is widely used as a laboratory model organism for research on photosynthesis, ciliary biology, lipid metabolism and many other aspects of cell biology and physiology. ...
    Small-scale DNA Extraction Method for Maize and Other Plants
    Author:  China Lunde, date: 03/20/2018, view: 3507, Q&A: 1
    Corn, or maize, is a major cereal crop and model monocot. Two of its benefits are ease of pollination and tractable genetics. For a comparison of development between maize and the dicot model, Arabidopsis, see Lunde and Hake, 2005. For an ...
    Zebrafish Embryo DNA Preparation
    Author:  Lili Jing, date: 02/20/2012, view: 17145, Q&A: 1
    This protocol explains how to extract DNA from a single zebrafish embryo. It does not require the use of expensive kits.
    Genotyping Transgenic Zebrafish Using Genomic DNA Extracted from Clutch of Embryos
    Author:  Lili Jing, date: 02/05/2012, view: 12191, Q&A: 0
    Transgenic zebrafish are very useful genetic tools to study various biological processes. Identification the right transgene founder and the subsequent transgenic animals are always tedious and time consuming. This protocol provides a relatively ...
    Chloroform-ethanol Isolation Genomic DNA from Mouse Tail
    Author:  Lin Fang, date: 09/20/2011, view: 10016, Q&A: 0
    This protocol describes a simple and fast method to purify genomic DNA from mouse tails using chloroform.
    E. coli Genomic DNA Extraction
    Author:  Fanglian He, date: 07/20/2011, view: 172365, Q&A: 52
    This protocol uses phenol/chloroform method to purify genomic DNA without using commercial kits.
    Extract Genomic DNA from Arabidopsis Leaves (Can be Used for Other Tissues as Well)
    Author:  Yongxian Lu, date: 07/05/2011, view: 24932, Q&A: 1
    This is a simple protocol for isolating genomic DNA from fresh plant tissues. DNA from this experiment can be used for all kinds of genetics studies, including genotyping and mapping. This protocol uses Edward’s extraction buffer to isolate DNA.