Molecular Biology

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    Safe DNA-extraction Protocol Suitable for Studying Tree-fungus Interactions
    Authors:  Susanna Keriö, Eeva Terhonen and Jared M. LeBoldus, date: 06/05/2020, view: 3221, Q&A: 0
    We present a safe and low-cost method suitable for DNA extraction from mycelium and tree tissue samples. After sample preparation, the extraction takes about 60 min. Method performance was tested by extracting DNA from various tree tissue samples ...
    Different Methods of Soil DNA Extraction
    Authors:  Asmita Kamble and Harinder Singh, date: 01/20/2020, view: 6709, Q&A: 0
    Soil is the major reservoir of microbial diversity. Only 1% of microbial diversity can be cultured while 99% is still not culturable. It is necessary to extract DNA from soil in order to explore the 99% microbial diversity, which will be useful to ...
    Simple Synthesis of Functionalized Paramagnetic Beads for Nucleic Acid Purification and Manipulation
    The purification of nucleic acids is one of the most common procedures employed in modern molecular biology laboratories. Typically, commercial column-based protocols are utilized to isolate DNA or RNA from various sources. However, these methods ...
    Isolation of PCR-quality Genomic DNA from Soils Impacted with Extra Heavy Crude Oil
    For the study of microbial communities in samples of soils impacted with extra heavy crude oil, it is necessary to perform molecular analyses. Due to the difficulty of oil matrix handling, there are very few protocols reported in writing. Also, one ...
    Fusarium graminearum Inoculation on Wheat Head
    Authors:  Chanjing Feng, Huiquan Liu and Zhe Tang, date: 08/05/2018, view: 5502, Q&A: 0
    Fusarium graminearum, the major causal agent of Fusarium head blight (FHB), causes serious wheat yield losses and a threat to human and animal health. The main efforts to combat the disease are the research of pathogenesis ...
    Plasmid Extract from Budding Yeast (Saccharomyces cerevisiae)
    Author:  Gal Haimovich, date: 07/20/2018, view: 7374, Q&A: 0
    Plasmids are widely used tools in yeast research. In many cases, plasmid libraries are used in genetic screens or in yeast two hybrid screens. In such cases, it is necessary to extract plasmids carrying unknown genetic elements from positive clones ...
    An Optimized CTAB Method for Genomic DNA Extraction from Freshly-picked Pinnae of Fern, Adiantum capillus-veneris L.
    Authors:  Yi Shu, Jin Wan-Ting, Yuan Ya-Ning and Fang Yu-Han, date: 07/05/2018, view: 13619, Q&A: 0
    As the sister clade of seed plants, ferns are significant materials for plant phylogeny research. However, the genomic DNA extraction protocol for fern samples like modified CTAB method still lacks robustness. Here, we found that the amount and ...
    Root Gall Formation, Resting Spore Isolation and High Molecular Weight DNA Extraction of Plasmodiophora brassicae
    Authors:  Sara Mehrabi, Suzana Stjelja and Christina Dixelius, date: 06/05/2018, view: 4732, Q&A: 0
    Isolation of DNA from obligate biotrophic soil-borne plant pathogens is challenging. This is because of their strict requirement of living plant tissue for their growth and propagation. A soil habitat further imposes risk of contamination from other ...
    Isolation of Genomic DNA from Chlamydomonas reinhardtii
    Author:  Tyler M Wittkopp, date: 05/05/2018, view: 10073, Q&A: 0
    Chlamydomonas reinhardtii is a soil-dwelling eukaryotic green alga that is widely used as a laboratory model organism for research on photosynthesis, ciliary biology, lipid metabolism and many other aspects of cell biology and physiology. ...
    Small-scale DNA Extraction Method for Maize and Other Plants
    Author:  China Lunde, date: 03/20/2018, view: 5725, Q&A: 1
    Corn, or maize, is a major cereal crop and model monocot. Two of its benefits are ease of pollination and tractable genetics. For a comparison of development between maize and the dicot model, Arabidopsis, see Lunde and Hake, 2005. For an ...



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