Biochemistry

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    Sucrose Gradient Analysis of Proteins
    Author:  Hui Zhu, date: 02/05/2012, view: 25934, Q&A: 1
    The sedimentation rate of a protein in a linear sucrose gradient can be used to determine its S value when compared to proteins of known S values which are run in a parallel gradient. This can be used to estimate an approximate molecular weight of ...
    Bradford Protein Assay
    Author:  Fanglian He, date: 03/20/2011, view: 375173, Q&A: 22
    The Bradford protein assay is used to measure the concentration of total protein in a sample. The principle of this assay is that the binding of protein molecules to Coomassie dye under acidic conditions results in a color change from brown to blue. ...
    BCA (Bicinchoninic Acid) Protein Assay
    Author:  Fanglian He, date: 03/05/2011, view: 137517, Q&A: 3
    The BCA protein assay is used for quantitation of total protein in a sample. The principle of this method is that proteins can reduce Cu+2 to Cu+1 in an alkaline solution (the biuret reaction) and result in a purple color ...
    Silver Staining
    Author:  Nabila Aboulaich, date: 02/05/2011, view: 30676, Q&A: 1



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