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A high-quality database for basic life science protocols
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A high-quality database for basic life science protocols
Plant Science
Latest Protocol Video
Using Seed Chipping to Genotype Maize Kernels
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Fluorescence Immunolocalization in Mitotic Chromosome Spreads from Maize Embryos
Authors:
Jing-Han Liu
,
Ya-zhong Wang
,
Lin Chen
and
Yan He
,
date:
06/20/2019,
view:
898,
Q&A:
0
Immunolocalization is a necessary technique in the study of chromosome structure and function. It can provide important information for better understanding the relation between epigenetic modifications and chromosome condensation. In this protocol, ...
Growing and Pollinating Maize
Authors:
Sarah Hake
and
China Lunde
,
date:
05/05/2018,
view:
2657,
Q&A:
0
[Abstract]
Corn, also known as maize, has been a model organism for genetics since the 1900’s due to the ease of pollinations, large ...
Isolation of Genomic DNA from
Chlamydomonas reinhardtii
Author:
Tyler M Wittkopp
,
date:
05/05/2018,
view:
6082,
Q&A:
0
Chlamydomonas reinhardtii
is a soil-dwelling eukaryotic green alga that is widely used as a laboratory model organism for research on photosynthesis, ciliary biology, lipid metabolism and many other aspects of cell biology and physiology. ...
Small-scale DNA Extraction Method for Maize and Other Plants
Author:
China Lunde
,
date:
03/20/2018,
view:
4055,
Q&A:
1
Corn, or maize, is a major cereal crop and model monocot. Two of its benefits are ease of pollination and tractable genetics. For a comparison of development between maize and the dicot model,
Arabidopsis
, see Lunde and Hake, 2005. For an ...
Extract Genomic DNA from
Arabidopsis
Leaves (Can be Used for Other Tissues as Well)
Author:
Yongxian Lu
,
date:
07/05/2011,
view:
25781,
Q&A:
1
This is a simple protocol for isolating genomic DNA from fresh plant tissues. DNA from this experiment can be used for all kinds of genetics studies, including genotyping and mapping. This protocol uses Edward’s extraction buffer to isolate DNA.
DNA Extraction from Dried Plant Tissues Using 96-well Format (cTab Method)
Author:
Yongxian Lu
,
date:
07/05/2011,
view:
15541,
Q&A:
5
This high throughput DNA isolation protocol is used to extract DNA of high quality from plant tissues for various genetics studies, like genotyping, and mapping etc. This protocol uses the well-established CTAB extraction procedure, and has been ...