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Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice

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    Barley Transformation by Particle Bombardment Using Callus
    Author:  Sung-Yong Kim, date: 06/05/2020, view: 1024, Q&A: 0
    The delivery of desired construct into plant cells relies mainly on Agrobacterium-mediated transformation. However, Agrobacterium transformation is still very challenging for barley as only one genotype is amendable to ...
    Fluorescence Immunolocalization in Mitotic Chromosome Spreads from Maize Embryos
    Authors:  Jing-Han Liu, Ya-zhong Wang, Lin Chen and Yan He, date: 06/20/2019, view: 1844, Q&A: 0
    Immunolocalization is a necessary technique in the study of chromosome structure and function. It can provide important information for better understanding the relation between epigenetic modifications and chromosome condensation. In this protocol, ...
    Growing and Pollinating Maize
    Authors:  Sarah Hake and China Lunde, date: 05/05/2018, view: 3548, Q&A: 0
    [Abstract] Corn, also known as maize, has been a model organism for genetics since the 1900’s due to the ease of pollinations, large ...
    Isolation of Genomic DNA from Chlamydomonas reinhardtii
    Author:  Tyler M Wittkopp, date: 05/05/2018, view: 9074, Q&A: 0
    Chlamydomonas reinhardtii is a soil-dwelling eukaryotic green alga that is widely used as a laboratory model organism for research on photosynthesis, ciliary biology, lipid metabolism and many other aspects of cell biology and physiology. ...
    Small-scale DNA Extraction Method for Maize and Other Plants
    Author:  China Lunde, date: 03/20/2018, view: 5325, Q&A: 1
    Corn, or maize, is a major cereal crop and model monocot. Two of its benefits are ease of pollination and tractable genetics. For a comparison of development between maize and the dicot model, Arabidopsis, see Lunde and Hake, 2005. For an ...
    Extract Genomic DNA from Arabidopsis Leaves (Can be Used for Other Tissues as Well)
    Author:  Yongxian Lu, date: 07/05/2011, view: 27824, Q&A: 2
    This is a simple protocol for isolating genomic DNA from fresh plant tissues. DNA from this experiment can be used for all kinds of genetics studies, including genotyping and mapping. This protocol uses Edward’s extraction buffer to isolate DNA.
    DNA Extraction from Dried Plant Tissues Using 96-well Format (cTab Method)
    Author:  Yongxian Lu, date: 07/05/2011, view: 16672, Q&A: 5
    This high throughput DNA isolation protocol is used to extract DNA of high quality from plant tissues for various genetics studies, like genotyping, and mapping etc. This protocol uses the well-established CTAB extraction procedure, and has been ...



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