Plant Science

Latest Protocol Video

Western Blot in Maize

Categories

    Fluorescence Immunolocalization in Mitotic Chromosome Spreads from Maize Embryos
    Authors:  Jing-Han Liu, Ya-zhong Wang, Lin Chen and Yan He, date: 06/20/2019, view: 314, Q&A: 0
    Immunolocalization is a necessary technique in the study of chromosome structure and function. It can provide important information for better understanding the relation between epigenetic modifications and chromosome condensation. In this protocol, ...
    Heat Induced Epitope Retrieval (HIER) Assisted Protein Immunostaining in Maize
    Authors:  Katsutoshi Tsuda and George Chuck, date: 06/05/2019, view: 262, Q&A: 0
    Protein immunostaining provides important spatio-temporal information about gene expression. Even using high quality antibodies, signal reproducibility and specificity can be problematic depending on tissue fixation methods. For example, ...
    Protein Immunoprecipitation in Maize
    Author:  María Jazmín Abraham-Juárez, date: 06/05/2019, view: 314, Q&A: 0
    Protein immunoprecipitation (IP) is a method used to identify cellular protein complexes. Whence, antibodies that specifically recognize a native bait protein or an epitope tag fused to the protein of interest allow the protein of interest to be ...
    A Transient Expression Assay Using Arabidopsis Mesophyll Protoplasts
    Author:  Xiyan Li, date: 05/20/2011, view: 15995, Q&A: 0
    This method can be used to free and separate the mesophyll cells from Arabidopsis leaves. The protoplasts that are generated in this way can be used for transient expression for protein activity and subcellular localization assays.