Plant Science

Immunolocalization is a necessary technique in the study of chromosome structure and function. It can provide important information for better understanding the relation between epigenetic modifications and chromosome condensation. In this protocol, ...

Protein immunostaining provides important spatio-temporal information about gene expression. Even using high quality antibodies, signal reproducibility and specificity can be problematic depending on tissue fixation methods. For example, ...

Protein immunoprecipitation (IP) is a method used to identify cellular protein complexes. Whence, antibodies that specifically recognize a native bait protein or an epitope tag fused to the protein of interest allow the protein of interest to be ...

This method can be used to free and separate the mesophyll cells from Arabidopsis leaves. The protoplasts that are generated in this way can be used for transient expression for protein activity and subcellular localization assays.