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Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice
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Fluorescence Immunolocalization in Mitotic Chromosome Spreads from Maize Embryos
Authors:
Jing-Han Liu
,
Ya-zhong Wang
,
Lin Chen
and
Yan He
,
date:
06/20/2019,
view:
1842,
Q&A:
0
Immunolocalization is a necessary technique in the study of chromosome structure and function. It can provide important information for better understanding the relation between epigenetic modifications and chromosome condensation. In this protocol, ...
Heat Induced Epitope Retrieval (HIER) Assisted Protein Immunostaining in Maize
Authors:
Katsutoshi Tsuda
and
George Chuck
,
date:
06/05/2019,
view:
1688,
Q&A:
0
Protein immunostaining provides important spatio-temporal information about gene expression. Even using high quality antibodies, signal reproducibility and specificity can be problematic depending on tissue fixation methods. For example, ...
Protein Immunoprecipitation in Maize
Author:
María Jazmín Abraham-Juárez
,
date:
06/05/2019,
view:
2013,
Q&A:
0
Protein immunoprecipitation (IP) is a method used to identify cellular protein complexes. Whence, antibodies that specifically recognize a native bait protein or an epitope tag fused to the protein of interest allow the protein of interest to be ...
A Transient Expression Assay Using
Arabidopsis
Mesophyll Protoplasts
Author:
Xiyan Li
,
date:
05/20/2011,
view:
18309,
Q&A:
0
This method can be used to free and separate the mesophyll cells from
Arabidopsis
leaves. The protoplasts that are generated in this way can be used for transient expression for protein activity and subcellular localization assays.
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