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A high-quality database for basic life science protocols
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A high-quality database for basic life science protocols
Immunology
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Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice
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Site Density Protocol
Author:
Jun Huang
,
date:
07/05/2012,
view:
7424,
Q&A:
0
The site densities of cell surface molecules are useful information for cell function analysis. Using antibody staining and commercial available calibration beads, this assay quantitatively determines the T cell receptor site density at the single T ...
In vitro
Differentiation of Mouse Th0, Th1 and Th2 from Naïve CD4 T Cells
Author:
Jia Li
,
date:
11/20/2011,
view:
53078,
Q&A:
20
In vitro
differentiation of helper T cells of various lineages is frequently used in T helper cell study. Naïve CD4 T cells can differentiate into certain lineage of T help cells
in vitro
in the presence of specific stimulatory ...
Naïve T Lymphocyte Infection by Murine Stem Cell Virus (MSCV) Protocol
Author:
Jia Li
,
date:
11/20/2011,
view:
11556,
Q&A:
0
This assay can be used for studying genes related to lymphocyte proliferation and differentiation
in vitro
. Stimulating lymphocytes to proliferate is important for the infection efficiency of MSCV. After overnight culture, if naïve cells ...
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