Biochemistry

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    A Lentiviral Pseudotype ELLA for the Measurement of Antibodies Against Influenza Neuraminidase
    Authors:  Fabrizio Biuso, George Carnell, Emanuele Montomoli and Nigel Temperton, date: 07/20/2018, view: 1675, Q&A: 0
    This protocol describes the rapid and safe production of lentiviral pseudotypes characterized by a lentiviral core containing a reporter, in conjunction with avian influenza haemagglutinin (HA) and human neuraminidase (NA) glycoproteins on the ...
    Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-histone Antibodies
    Author:  Zheng Liu, date: 06/20/2012, view: 8733, Q&A: 0
    Autoimmune disease is characterized with the break of tolerance against self antigens. Anti-histone antibodies can be found in the majority of patients with system lupus erythematosus (SLE) and a number of lupus-prone mouse strains. This protocol ...
    RNP-IP (Original Method): Obtaining Majority RNA from RNA Binding Protein in the Nucleus
    Author:  FengZhi Liu, date: 05/20/2012, view: 15745, Q&A: 1
    Post-transcriptional regulation of gene expression is a ribonucleoprotein (RNP)-driven process, which involves RNA binding proteins (RBPs) and noncoding RNAs that regulate splicing, nuclear export, subcellular localization, mRNA stability and ...
    Immunofluorescence Detection and F-actin Staining of MTLn3 Cells
    Author:  Huan Pang, date: 04/20/2012, view: 12063, Q&A: 0
    Epidermal growth factor (EGF)-stimulated MTLn3 cells protrusion play an important role in cell migration. Phalloidin which binds F-actin in cells is an imaging tool used with light microscopy to investigate the distribution of actin. The protocol ...
    Flourescent Immunostaining Protocol for a-Bungorotoxin (AChRs) in Zebrafish
    Author:  Lili Jing, date: 02/20/2012, view: 10204, Q&A: 0
    Zebrafish embryo is convenient for studying neuromuscular system due to its transparency. This protocol is a rapid method to visualize the AChR clusters on the postsynaptic muscle membrane.
    A General Protocol for GST Pull-down
    Author:  Lili Jing, date: 01/20/2012, view: 60508, Q&A: 3
    GST-Pull down assay is an effective way to examine the direct binding of two proteins in vitro. This protocol is based on GST pull down system from GE healthcare, and uses the binding of unplugged/MuSK receptor and Wnt ligand as an example ...
    Crosslinking and Immunoprecipitation in Zebrafish
    Author:  Lili Jing, date: 01/20/2012, view: 11028, Q&A: 0
    Immunoprecipitation (IP) is a routine method to detect protein binding and interactions. But the weak binding between two proteins is often hard to detect during regular IP procedure. This protocol offers a crossliking and IP combination method to ...
    ELISA Measurement of Mouse IL-2
    Author:  Guo N. Huang, date: 01/05/2012, view: 8330, Q&A: 0
    Interleukin-2 (IL-2) is a cytokine secreted by T cells that is essential for immune system activation. This protocol is routinely used for quantification of IL-2 concentration in the supernant of cultured lymphocytes under various stimulations and ...
    qPCR of Yeast ChIP DNA
    Author:  Wei Zheng, date: 09/20/2011, view: 13123, Q&A: 1
    This protocol is adapted from Chris Yellman's personal notes for qPCR of yeast ChIP DNA to verify binding sites already identified by microarray/sequencing analysis. In addition, qPCR is a quick way to assay the quality of a ChIP sample ...
    Western Blot for Detecting Phosphorylated STAT3
    Author:  Huagang Zhang, date: 08/20/2011, view: 19002, Q&A: 0
    The STAT3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors and is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. STAT3 is ...