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Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice

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Western Blot in Maize

Author: María Jazmín Abraham-Juárez, date: 06/05/2019, view: 5288, Q&A: 0

Since its introduction in 1979 (Towbin et al., 1979), protein blotting (Western blot) has become a routine tool in molecular biology research. It is used to detect low amounts of proteins in complex samples or to monitor protein expression ...

A Lentiviral Pseudotype ELLA for the Measurement of Antibodies Against Influenza Neuraminidase

Authors: Fabrizio Biuso, George Carnell, Emanuele Montomoli and Nigel Temperton, date: 07/20/2018, view: 4126, Q&A: 0

This protocol describes the rapid and safe production of lentiviral pseudotypes characterized by a lentiviral core containing a reporter, in conjunction with avian influenza haemagglutinin (HA) and human neuraminidase (NA) glycoproteins on the ...

Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-histone Antibodies

Author: Zheng Liu, date: 06/20/2012, view: 10708, Q&A: 0

Autoimmune disease is characterized with the break of tolerance against self antigens. Anti-histone antibodies can be found in the majority of patients with system lupus erythematosus (SLE) and a number of lupus-prone mouse strains. This protocol ...

RNP-IP (Original Method): Obtaining Majority RNA from RNA Binding Protein in the Nucleus

Author: FengZhi Liu, date: 05/20/2012, view: 18072, Q&A: 1

Post-transcriptional regulation of gene expression is a ribonucleoprotein (RNP)-driven process, which involves RNA binding proteins (RBPs) and noncoding RNAs that regulate splicing, nuclear export, subcellular localization, mRNA stability and ...

Immunofluorescence Detection and F-actin Staining of MTLn3 Cells

Author: Huan Pang, date: 04/20/2012, view: 14381, Q&A: 0

Epidermal growth factor (EGF)-stimulated MTLn3 cells protrusion play an important role in cell migration. Phalloidin which binds F-actin in cells is an imaging tool used with light microscopy to investigate the distribution of actin. The protocol ...

Flourescent Immunostaining Protocol for a-Bungorotoxin (AChRs) in Zebrafish

Author: Lili Jing, date: 02/20/2012, view: 11878, Q&A: 0

Zebrafish embryo is convenient for studying neuromuscular system due to its transparency. This protocol is a rapid method to visualize the AChR clusters on the postsynaptic muscle membrane.

A General Protocol for GST Pull-down

Author: Lili Jing, date: 01/20/2012, view: 71745, Q&A: 3

GST-Pull down assay is an effective way to examine the direct binding of two proteins in vitro. This protocol is based on GST pull down system from GE healthcare, and uses the binding of unplugged/MuSK receptor and Wnt ligand as an example ...

Crosslinking and Immunoprecipitation in Zebrafish

Author: Lili Jing, date: 01/20/2012, view: 13218, Q&A: 0

Immunoprecipitation (IP) is a routine method to detect protein binding and interactions. But the weak binding between two proteins is often hard to detect during regular IP procedure. This protocol offers a crossliking and IP combination method to ...

ELISA Measurement of Mouse IL-2

Author: Guo N. Huang, date: 01/05/2012, view: 10499, Q&A: 0

Interleukin-2 (IL-2) is a cytokine secreted by T cells that is essential for immune system activation. This protocol is routinely used for quantification of IL-2 concentration in the supernant of cultured lymphocytes under various stimulations and ...

qPCR of Yeast ChIP DNA

Author: Wei Zheng, date: 09/20/2011, view: 15769, Q&A: 1

This protocol is adapted from Chris Yellman's personal notes for qPCR of yeast ChIP DNA to verify binding sites already identified by microarray/sequencing analysis. In addition, qPCR is a quick way to assay the quality of a ChIP sample ...

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