Home
About
For Authors
Submit Protocol
Request a Protocol
My bio page
Reset the password
Home
About
For Authors
Submit Protocol
Request a Protocol
A high-quality database for basic life science protocols
Search
Biochemistry
Latest Protocol Video
Mating and Tetrad Dissection in
Chlamydomonas
Categories
By Date
By View
A Lentiviral Pseudotype ELLA for the Measurement of Antibodies Against Influenza Neuraminidase
Authors:
Fabrizio Biuso
,
George Carnell
,
Emanuele Montomoli
and
Nigel Temperton
,
date:
07/20/2018,
view:
1675,
Q&A:
0
This protocol describes the rapid and safe production of lentiviral pseudotypes characterized by a lentiviral core containing a reporter, in conjunction with avian influenza haemagglutinin (HA) and human neuraminidase (NA) glycoproteins on the ...
Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-histone Antibodies
Author:
Zheng Liu
,
date:
06/20/2012,
view:
8733,
Q&A:
0
Autoimmune disease is characterized with the break of tolerance against self antigens. Anti-histone antibodies can be found in the majority of patients with system lupus erythematosus (SLE) and a number of lupus-prone mouse strains. This protocol ...
RNP-IP (Original Method): Obtaining Majority RNA from RNA Binding Protein in the Nucleus
Author:
FengZhi Liu
,
date:
05/20/2012,
view:
15745,
Q&A:
1
Post-transcriptional regulation of gene expression is a ribonucleoprotein (RNP)-driven process, which involves RNA binding proteins (RBPs) and noncoding RNAs that regulate splicing, nuclear export, subcellular localization, mRNA stability and ...
Immunofluorescence Detection and F-actin Staining of MTLn3 Cells
Author:
Huan Pang
,
date:
04/20/2012,
view:
12063,
Q&A:
0
Epidermal growth factor (EGF)-stimulated MTLn3 cells protrusion play an important role in cell migration. Phalloidin which binds F-actin in cells is an imaging tool used with light microscopy to investigate the distribution of actin. The protocol ...
Flourescent Immunostaining Protocol for a-Bungorotoxin (AChRs) in Zebrafish
Author:
Lili Jing
,
date:
02/20/2012,
view:
10204,
Q&A:
0
Zebrafish embryo is convenient for studying neuromuscular system due to its transparency. This protocol is a rapid method to visualize the AChR clusters on the postsynaptic muscle membrane.
A General Protocol for GST Pull-down
Author:
Lili Jing
,
date:
01/20/2012,
view:
60508,
Q&A:
3
GST-Pull down assay is an effective way to examine the direct binding of two proteins
in vitro
. This protocol is based on GST pull down system from GE healthcare, and uses the binding of unplugged/MuSK receptor and Wnt ligand as an example ...
Crosslinking and Immunoprecipitation in Zebrafish
Author:
Lili Jing
,
date:
01/20/2012,
view:
11028,
Q&A:
0
Immunoprecipitation (IP) is a routine method to detect protein binding and interactions. But the weak binding between two proteins is often hard to detect during regular IP procedure. This protocol offers a crossliking and IP combination method to ...
ELISA Measurement of Mouse IL-2
Author:
Guo N. Huang
,
date:
01/05/2012,
view:
8330,
Q&A:
0
Interleukin-2 (IL-2) is a cytokine secreted by T cells that is essential for immune system activation. This protocol is routinely used for quantification of IL-2 concentration in the supernant of cultured lymphocytes under various stimulations and ...
qPCR of Yeast ChIP DNA
Author:
Wei Zheng
,
date:
09/20/2011,
view:
13123,
Q&A:
1
This protocol is adapted from Chris Yellman's personal notes for qPCR of yeast ChIP DNA to verify binding sites already identified by microarray/sequencing analysis. In addition, qPCR is a quick way to assay the quality of a ChIP sample ...
Western Blot for Detecting Phosphorylated STAT3
Author:
Huagang Zhang
,
date:
08/20/2011,
view:
19002,
Q&A:
0
The STAT3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors and is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. STAT3 is ...