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Biochemistry
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Western Blot in Maize
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Western Blot in Maize
Author:
María Jazmín Abraham-Juárez
,
date:
06/05/2019,
view:
870,
Q&A:
0
Since its introduction in 1979 (Towbin
et al.
, 1979), protein blotting (Western blot) has become a routine tool in molecular biology research. It is used to detect low amounts of proteins in complex samples or to monitor protein expression ...
A Lentiviral Pseudotype ELLA for the Measurement of Antibodies Against Influenza Neuraminidase
Authors:
Fabrizio Biuso
,
George Carnell
,
Emanuele Montomoli
and
Nigel Temperton
,
date:
07/20/2018,
view:
2205,
Q&A:
0
This protocol describes the rapid and safe production of lentiviral pseudotypes characterized by a lentiviral core containing a reporter, in conjunction with avian influenza haemagglutinin (HA) and human neuraminidase (NA) glycoproteins on the ...
Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-histone Antibodies
Author:
Zheng Liu
,
date:
06/20/2012,
view:
9140,
Q&A:
0
Autoimmune disease is characterized with the break of tolerance against self antigens. Anti-histone antibodies can be found in the majority of patients with system lupus erythematosus (SLE) and a number of lupus-prone mouse strains. This protocol ...
RNP-IP (Original Method): Obtaining Majority RNA from RNA Binding Protein in the Nucleus
Author:
FengZhi Liu
,
date:
05/20/2012,
view:
16445,
Q&A:
1
Post-transcriptional regulation of gene expression is a ribonucleoprotein (RNP)-driven process, which involves RNA binding proteins (RBPs) and noncoding RNAs that regulate splicing, nuclear export, subcellular localization, mRNA stability and ...
Immunofluorescence Detection and F-actin Staining of MTLn3 Cells
Author:
Huan Pang
,
date:
04/20/2012,
view:
12691,
Q&A:
0
Epidermal growth factor (EGF)-stimulated MTLn3 cells protrusion play an important role in cell migration. Phalloidin which binds F-actin in cells is an imaging tool used with light microscopy to investigate the distribution of actin. The protocol ...
Flourescent Immunostaining Protocol for a-Bungorotoxin (AChRs) in Zebrafish
Author:
Lili Jing
,
date:
02/20/2012,
view:
10625,
Q&A:
0
Zebrafish embryo is convenient for studying neuromuscular system due to its transparency. This protocol is a rapid method to visualize the AChR clusters on the postsynaptic muscle membrane.
A General Protocol for GST Pull-down
Author:
Lili Jing
,
date:
01/20/2012,
view:
64012,
Q&A:
3
GST-Pull down assay is an effective way to examine the direct binding of two proteins
in vitro
. This protocol is based on GST pull down system from GE healthcare, and uses the binding of unplugged/MuSK receptor and Wnt ligand as an example ...
Crosslinking and Immunoprecipitation in Zebrafish
Author:
Lili Jing
,
date:
01/20/2012,
view:
11542,
Q&A:
0
Immunoprecipitation (IP) is a routine method to detect protein binding and interactions. But the weak binding between two proteins is often hard to detect during regular IP procedure. This protocol offers a crossliking and IP combination method to ...
ELISA Measurement of Mouse IL-2
Author:
Guo N. Huang
,
date:
01/05/2012,
view:
8790,
Q&A:
0
Interleukin-2 (IL-2) is a cytokine secreted by T cells that is essential for immune system activation. This protocol is routinely used for quantification of IL-2 concentration in the supernant of cultured lymphocytes under various stimulations and ...
qPCR of Yeast ChIP DNA
Author:
Wei Zheng
,
date:
09/20/2011,
view:
13807,
Q&A:
1
This protocol is adapted from Chris Yellman's personal notes for qPCR of yeast ChIP DNA to verify binding sites already identified by microarray/sequencing analysis. In addition, qPCR is a quick way to assay the quality of a ChIP sample ...