Plant Science

Categories

    Acidified Blue Ink-staining Procedure for the Observation of Fungal Structures Inside Roots of Two Disparate Plant Lineages
    Authors:  Jill Kowal, Elena Arrigoni and Sophie Lane, date: 10/20/2020, view: 1500, Q&A: 0
    Identifying microscopic mycorrhizal fungal structures in roots, i.e., hyphae, vesicles and arbuscules, requires root staining procedures that are often time consuming and involves chemicals known to present health risks from exposure. By ...
    Visualization of Actin Organization and Quantification in Fixed Arabidopsis Pollen Grains and Tubes
    Authors:  Xiaolu Qu, Qiannan Wang, Haiyan Wang and Shanjin Huang, date: 01/05/2020, view: 2625, Q&A: 0
    Although it is widely accepted that actin plays an important role in regulating pollen germination and pollen tube growth, how actin exactly performs functions remains incompletely understood. As the function of actin is dictated by its spatial ...
    Artificial Inoculation of Epichloë festucae into Lolium perenne, and Visualisation of Endophytic and Epiphyllous Fungal Growth
    Authors:  Yvonne Becker, Kimberly A. Green, Barry Scott and Matthias Becker, date: 09/05/2018, view: 5796, Q&A: 0
    Natural hosts for the fungal endophyte Epichloë festucae include Festuca rubra (fine fescue) and Festuca trachyphylla (hard fescue). Some strains also form stable associations with Lolium perenne (perennial ryegrass). L. ...
    Analysis of Chromosome Condensation/Decondensation During Mitosis by EdU Incorporation in Nigella damascena L. Seedling Roots
    Author:  Eugene V. Sheval, date: 02/05/2018, view: 4506, Q&A: 0
    To investigate the chromosome dynamics during mitosis, it is convenient to mark the discrete chromosome foci and then analyze their spatial rearrangements during prophase condensation and telophase decondensation. To label the chromosome regions in ...
    Determination of H+-ATPase Activity in Arabidopsis Guard Cell Protoplasts through H+-pumping Measurement and H+-ATPase Quantification
    Authors:  Shota Yamauchi and Ken-ichiro Shimazaki, date: 12/20/2017, view: 6759, Q&A: 0
    The opening of stomata in plants in response to blue light is driven by the plasma membrane H+-ATPase in guard cells. To evaluate the activation of the H+-ATPase in vivo, we can use H+-pumping by guard cells ...
    Quantification of Membrane Damage/Cell Death Using Evan’s Blue Staining Technique
    Membrane damage is a hallmark of both biotic and abiotic stress responses. The membrane determines the ability of a cell to sustain altered environmental conditions and hence can be used as a biomarker to assess stress-induced cell damage or death. ...
    Use of SCRI Renaissance 2200 (SR2200) as a Versatile Dye for Imaging of Developing Embryos, Whole Ovules, Pollen Tubes and Roots
    Authors:  Thomas J. Musielak, Patrick Bürgel, Martina Kolb and Martin Bayer, date: 09/20/2016, view: 13150, Q&A: 1
    Confocal laser scanning microscopy in combination with fluorescent proteins is a powerful tool for the study of sexual reproduction and other developmental processes in plants. In order to understand the origin and localization of fluorescent ...
    Quantification of Callose Deposition in Plant Leaves
    Callose is an amorphous homopolymer, composed of β-1, 3-glucan, which is widespread in higher plants. Callose is involved in multiple aspects of plant growth and development. It is synthetized in plants at the cell plate during cytokinesis, in ...
    Histochemical Staining of Silica Body in Rice Leaf Blades
    Silicon (Si) is a biologically important element for plants in the order Poales (Yamamoto et al., 2011; Kido et al., 2015). In rice, Si is mainly deposited in the motor cells and the cell walls of the leaf epidermis. However, the ...
    Detection of Poly (A) RNA in Mesophyll Cells of Nicotiana benthamiana Using in situ Hybridization
    Authors:  Yuri Mizuno and Daigo Takemoto, date: 09/05/2015, view: 7067, Q&A: 0
    Export of transcribed mRNAs from nucleoplasm to cytosol is an essential process for the translation of genes into proteins. This process is tightly regulated by nuclear pores, composed of about 30 nucleoporin proteins (Nups). Whether or not the ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.