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Transcardiac Perfusion of the Mouse for Brain Tissue Dissection and Fixation

JW Jinyun Wu YC Yuqi Cai XW Xiaoyang Wu YY Yue Ying YT Yilin Tai

Miao He*

0 Q&A 13489 Views Mar 5, 2021

Transcardiac perfusion with saline followed by 4% paraformaldehyde (PFA) is widely used to clear blood and preserve brain for immunostaining or in situ hybridization. PFA breaks into formaldehyde in solution, which cross-link protein and DNA molecules to preserve tissue and cell structure. Here we provide a step by step guide for performing this procedure in mouse.

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