Cell Biology

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    Trichloroacetic Acid Fixation and Antibody Staining of Zebrafish Larvae
    Authors:  E. Anne Martin, Sundas Ijaz, Alberto E. Pereda and Adam C. Miller, date: 01/20/2022, view: 38, Q&A: 0

    Larval zebrafish have been established as an excellent model for examining vertebrate biology, with many researchers using the system for neuroscience. Controlling a fast escape response of the fish, the Mauthner cells and their associated network

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    Whole-mount Immunohistochemistry to Visualize Mouse Embryonic Dermal Lymphatic Vasculature
    Authors:  Xin Geng and R. Sathish Srinivasan, date: 10/20/2021, view: 580, Q&A: 0

    Lymphatic vessels are abundant in the skin where they regulate interstitial fluid uptake and immune surveillance. Defects in dermal lymphatic vessels, such as fewer vessels and abnormal lymphatic vessel coverage with mural cells, are frequently

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    Phalloidin Staining of Actin Filaments for Visualization of Muscle Fibers in Caenorhabditis elegans
    Authors:  Mario Romani and Johan Auwerx, date: 10/05/2021, view: 1330, Q&A: 0

    Advances in C. elegans research have allowed scientists to recapitulate different human disorders, from neurodegenerative diseases to muscle dysfunction, in these nematodes. Concomitantly, the interest in visualizing organs affected by these

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    Clearing of the Mouse Brain for Optical Imaging Using CUBIC
    Authors:  Lianxiang Luo, Xiaoyu Chen, Ruijian Wu, Han Wang and Hui Luo, date: 08/20/2021, view: 592, Q&A: 0

    Here, we describe a simple and efficient tissue clearing method, CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis), which was first introduced in 2014 (Susaki et al., 2014). In the present study, the hemispheres of

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    Optical Clearing and 3D Analysis Optimized for Mouse and Human Pancreata

    The pancreas is a heavily innervated organ, but pancreatic innervation can be challenging to comprehensively assess using conventional histological methods. However, recent advances in whole-mount tissue clearing and 3D rendering techniques have

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    Brain-localized and Intravenous Microinjections in the Larval Zebrafish to Assess Innate Immune Response
    Authors:  Alison M. Rojas and Celia E. Shiau, date: 04/05/2021, view: 2673, Q&A: 0

    Creating a robust and controlled infection model is imperative for studying the innate immune response. Leveraging the particular strengths of the zebrafish model system, such as optical transparency, ex utero development, and large clutch size,

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    Measuring Bone Volume at Multiple Densities by Micro-computed Tomography
    Authors:  Emma C. Walker, Narelle E. McGregor, Audrey S. M. Chan and Natalie A. Sims, date: 01/05/2021, view: 2042, Q&A: 0

    Bone strength is controlled by both bone mass, and the organization and quality of the bone material. The current standard method for measuring bone mass in mouse and rat studies is micro-computed tomography. This method typically uses a single

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    Mechanical Tissue Compression and Whole-mount Imaging at Single Cell Resolution for Developing Murine Epididymal Tubules
    Author:  Tsuyoshi Hirashima, date: 08/05/2020, view: 2344, Q&A: 0
    Cells inside the body are subjected to various mechanical stress, such as stretch or compression provided by surrounding cells, shear stresses by blood or lymph flows, and normal stresses by luminal liquids. Force loading to the biological tissues ...
    Toluidine Blue O Staining of Paraffin-Sectioned Maize Tissue
    Authors:  Josh Strable, Jeffrey R. Yen, Michael J. Scanlon and Anne W. Sylvester, date: 05/05/2020, view: 2739, Q&A: 0
    Histology incorporates microtechnique, the preparation of living material for observation and microscopy to study the anatomy of cells, tissues and organs. Interpreting histological patterns in a specimen may help to correlate form and function at ...
    Method for Measuring Mucociliary Clearance and Cilia-generated Flow in Mice by ex vivo Imaging
    Authors:  Eric Song and Akiko Iwasaki, date: 03/20/2020, view: 2806, Q&A: 0
    Ex vivo biophysical measurements provide valuable insights into understanding both physiological and pathogenic processes. One critical physiological mechanism that is regulated by these biophysical properties is cilia-generated flow that ...



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