Systems Biology


    Protein-ligand Binding Assay by Liquid Chromatography-Mass Spectrometry
    Author:  Xiyan Li, date: 08/20/2011, view: 15012, Q&A: 0
    Protein-small molecule binding coefficients are determined by quantitative LC-MS in this method. Traditional biochemical plot is consequently used to set up the binding curve between a known protein and a small molecule (<1000 da) to determine the affinity constant (kd) and the stoichiometry (bmax). da)="" to="" determine="" the="" affinity="" constant="" (kd)="" and="" the="" stoichiometry=""> ...

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