Immunology

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Western Blot in Maize

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    Immunohistochemistry Staining for Tumor-associated Macrophage Polarization in Murine Subcutaneous Colon Tumor Allografts
    Authors:  Gage J. Greening, Shelby N. Bess and Timothy J. Muldoon, date: 12/05/2018, view: 2364, Q&A: 0
    Tumor-associated macrophages are the most abundant immune cells in the tumor microenvironment and may express both anti-tumor and pro-tumor functions depending on their polarization towards an M1 (classical) or M2 (alternative) phenotype, ...
    Thioglycollate Induced Peritonitis
    Author:  Zheng Liu, date: 06/20/2011, view: 27027, Q&A: 3
    Intraperitoneal (i.p.) injection of thioglycollate elicits a robust influx of neutrophils into peritoneal cavity. The trafficking of the cells is believed to be mediated by chemokines CXCL1, CXCL2, and CXCL8 (Call et al., 2001; Cacalano et ...
    Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-dsDNA Antibodies
    Author:  Zheng Liu, date: 02/20/2011, view: 12330, Q&A: 0
    ELISA is an easy and relatively sensitive way to measure protein concentration. This protocol describes how to measure serum levels of murine anti-dsDNA antibodies. Circulating anti-dsDNA antibody is a hallmark of SLE both in human patients and in ...