Plant Science

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Western Blot in Maize

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    Hormone Treatments during Maize Vegetative and Reproductive Development
    Authors:  María Jazmín Abraham-Juárez and Harry Klein, date: 05/20/2019, view: 194, Q&A: 0
    Plant hormones are a group of organic natural compounds that can influence physiological processes when present at low concentrations. Plant biologists frequently use exogenous hormone application to modify plant physiology or to rescue mutants with ...
    A Simplified Hydroponic Culture of Arabidopsis
    Authors:  Houqing Zeng, Chao Xia, Cankui Zhang and Li-Qing Chen, date: 12/20/2018, view: 1667, Q&A: 0
    Hydroponic culture systems are widely used in research due to their intrinsic properties, such as easily altering nutrient composition, applying chemical treatments like metal, salt and hormones in the growth media, and collecting the root sample. ...
    Screening and Genetic Analysis of Ethylene-Response Mutants in Etiolated Rice Seedlings
    Authors:  Cui-Cui Yin, Biao Ma, He Zhao, Shou-Yi Chen and Jin-Song Zhang, date: 09/05/2018, view: 1428, Q&A: 0
    Ethylene, the simplest gaseous plant phytohormone, is involved in the control of rice growth and development processes, but the mechanism of ethylene regulating these pathways remains unclear in rice. Recent studies have found that ...
    Growth of Chlamydomonas reinhardtii under Circadian Conditions
    Authors:  Volker Wagner and Maria Mittag, date: 08/20/2018, view: 1688, Q&A: 0
    The green biflagellate unicellular alga Chlamydomonas reinhardtii serves as a model to study fundamental biological processes such as the structure and function of flagella or light-driven processes including photosynthesis, its behavioral ...
    Protocol to Test the Effect of Sorbitol in vitro on Hybrid Larch (Larix x eurolepis Henry) Emblings
    Authors:  Bettina Johst and María del Carmen Dacasa Rüdinger, date: 07/20/2018, view: 1401, Q&A: 0
    This protocol presents a method to test the effect of sorbitol in vitro on hybrid larch plants derived from somatic embryogenesis. We have tested four different media variants, one control variant without sorbitol and three variants of ...
    Root Gall Formation, Resting Spore Isolation and High Molecular Weight DNA Extraction of Plasmodiophora brassicae
    Authors:  Sara Mehrabi, Suzana Stjelja and Christina Dixelius, date: 06/05/2018, view: 2029, Q&A: 0
    Isolation of DNA from obligate biotrophic soil-borne plant pathogens is challenging. This is because of their strict requirement of living plant tissue for their growth and propagation. A soil habitat further imposes risk of contamination from other ...
    Growing and Pollinating Maize
    Authors:  Sarah Hake and China Lunde, date: 05/05/2018, view: 1881, Q&A: 0
    [Abstract] Corn, also known as maize, has been a model organism for genetics since the 1900’s due to the ease of pollinations, large ...
    Arabidopsis Growing Protocol – A General Guide
    Author:  Xiyan Li, date: 09/05/2011, view: 24273, Q&A: 1
    Arabidopsis as the model organism for higher plants is widely studied among plant biology labs around the world. However, taking care of this tiny plant may not be trivial. Here is a general guide used for the Heven Sze lab at the University of ...
    Arabidopsis Stomatal Opening and Closing Experiment
    Author:  Zhixin Zhao, date: 05/20/2011, view: 14142, Q&A: 1
    Stomata pores are mainly localized in the lower epidermis of plant leaves and transpirational water loss occurs through these pores. ABA inhibits light-promoted stomatal opening and promotes stomatal closure. This protocol describes how to measure ...
    Arabidopsis Pollen Tube Germination
    Author:  Xiyan Li, date: 05/20/2011, view: 15668, Q&A: 0
    This method uses a PEG-supplemented liquid solution to germinate separated Aradidopsis pollen. It thus eliminated the need for humidity control.