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Chiasma Counting in Maize Male Meiocytes

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A Low-volume Hydroponic Protocol for Maize and A Sensitive Bud-length Assay for Root-to-Shoot Impacts of The Strigolactone Analog, rac-GR24

Authors: Jiahn-Chou Guan and Karen E. Koch, date: 08/20/2020, view: 191, Q&A: 0

Hydroponic approaches have been a boon for root research by facilitating root-feeding studies as well as secretion analysis. Results have aided our understanding of root-shoot signaling, transport, hormone function and more. However, existing ...

In vitro Conditions for Dark Growth and Analysis of Maize Seedlings

Authors: Jeffrey Farrow, Marschal A. Bellinger and Carolyn G. Rasmussen, date: 03/20/2020, view: 704, Q&A: 0

Maize is an important model organism for understanding plant traits essential for proper growth and germination. One type of growth, skotomorphogenesis, occurs in the absence of light. Seedlings grown in the absence of light exhibit dramatic ...

Quantifying MAMP-induced Production of Reactive Oxygen Species in Sorghum and Maize

Authors: Rozalynne Samira, Xinye Zhang, Jennifer Kimball, Yaya Cui, Gary Stacey and Peter J. Balint-Kurti, date: 07/20/2019, view: 1540, Q&A: 0

Plants have the ability to recognize microbe-associated molecular patterns (MAMPs) and mount a defense response. The level of the MAMP response can vary depending on genetic and environmental factors. The most commonly studied MAMPs are flg22, a ...

Hormone Treatments during Maize Vegetative and Reproductive Development

Authors: María Jazmín Abraham-Juárez and Harry Klein, date: 05/20/2019, view: 1566, Q&A: 0

Plant hormones are a group of organic natural compounds that can influence physiological processes when present at low concentrations. Plant biologists frequently use exogenous hormone application to modify plant physiology or to rescue mutants with ...

A Simplified Hydroponic Culture of Arabidopsis

Authors: Houqing Zeng, Chao Xia, Cankui Zhang and Li-Qing Chen, date: 12/20/2018, view: 5412, Q&A: 0

Hydroponic culture systems are widely used in research due to their intrinsic properties, such as easily altering nutrient composition, applying chemical treatments like metal, salt and hormones in the growth media, and collecting the root sample. ...

Screening and Genetic Analysis of Ethylene-Response Mutants in Etiolated Rice Seedlings

Authors: Cui-Cui Yin, Biao Ma, He Zhao, Shou-Yi Chen and Jin-Song Zhang, date: 09/05/2018, view: 2812, Q&A: 0

Ethylene, the simplest gaseous plant phytohormone, is involved in the control of rice growth and development processes, but the mechanism of ethylene regulating these pathways remains unclear in rice. Recent studies have found that ...

Growth of Chlamydomonas reinhardtii under Circadian Conditions

Authors: Volker Wagner and Maria Mittag, date: 08/20/2018, view: 3341, Q&A: 0

The green biflagellate unicellular alga Chlamydomonas reinhardtii serves as a model to study fundamental biological processes such as the structure and function of flagella or light-driven processes including photosynthesis, its behavioral ...

Protocol to Test the Effect of Sorbitol in vitro on Hybrid Larch (Larix x eurolepis Henry) Emblings

Authors: Bettina Johst and María del Carmen Dacasa Rüdinger, date: 07/20/2018, view: 2565, Q&A: 0

This protocol presents a method to test the effect of sorbitol in vitro on hybrid larch plants derived from somatic embryogenesis. We have tested four different media variants, one control variant without sorbitol and three variants of ...

Root Gall Formation, Resting Spore Isolation and High Molecular Weight DNA Extraction of Plasmodiophora brassicae

Authors: Sara Mehrabi, Suzana Stjelja and Christina Dixelius, date: 06/05/2018, view: 3796, Q&A: 0

Isolation of DNA from obligate biotrophic soil-borne plant pathogens is challenging. This is because of their strict requirement of living plant tissue for their growth and propagation. A soil habitat further imposes risk of contamination from other ...

Growing and Pollinating Maize

Authors: Sarah Hake and China Lunde, date: 05/05/2018, view: 3167, Q&A: 0

[Abstract] Corn, also known as maize, has been a model organism for genetics since the 1900’s due to the ease of pollinations, large ...

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