Home About Us

Cancer Biology

Latest Protocol Video

Chiasma Counting in Maize Male Meiocytes

Categories

By Date By View

A Rigorous Quantitative Approach to Analyzing Phagocytosis Assays

Authors: Michael D. Caponegro, Kaitlyn Koenig Thompson, Maryam Tayyab and Stella E. Tsirka, date: 08/05/2020, view: 57, Q&A: 0

Studying monocytic cells in isolated systems in vitro contributes significantly to the understanding of innate immune physiology. Functional assays produce read outs which can be used to measure responses to selected stimuli, such as ...

Cellular Retention Assay to Determine the Interaction Frequency of CD19-directed Chimeric Antigen Receptor (CAR) Engineered Cells against CD19⁺ Leukemic Cells

Authors: Marvin I. De los Santos and Samuel D. Bernal, date: 09/05/2019, view: 1283, Q&A: 0

Cancer recognition by chimeric antigen receptor T (CAR-T) cell is prerequisite for cancer killing to occur. Anomalies in the binding specificity of scFv in the CAR construct may prevent successful eradication of these cancer cells. In some cases, ...

Immunohistochemistry Staining for Tumor-associated Macrophage Polarization in Murine Subcutaneous Colon Tumor Allografts

Authors: Gage J. Greening, Shelby N. Bess and Timothy J. Muldoon, date: 12/05/2018, view: 4928, Q&A: 0

Tumor-associated macrophages are the most abundant immune cells in the tumor microenvironment and may express both anti-tumor and pro-tumor functions depending on their polarization towards an M1 (classical) or M2 (alternative) phenotype, ...

Quantifying Gene Expression Directly from FACS Using Hydrolysis (TaqMan) Probes on Flash-frozen Cells

Authors: Kirsten A. Copren, Oyang Teng, Cassandra J. Adams, Cláudia Bispo and Michael Lee, date: 04/20/2018, view: 4592, Q&A: 0

The TaqMan Gene Expression Cells-to-Ct^TM Kit enables reverse transcription directly from cell lysates without the need for isolating RNA. The recommended input range for the lysis reaction is between 10-10⁵ cells, though the ...

Purifying Properly Folded Cysteine-rich, Zinc Finger Containing Recombinant Proteins for Structural Drug Targeting Studies: the CH1 Domain of p300 as a Case Example

Authors: Yong Joon Kim, Stefan Kaluz, Anil Mehta, Emily Weinert, Shannon Rivera and Erwin G. Van Meir, date: 09/05/2017, view: 8354, Q&A: 0

The transcription factor Hypoxia-Inducible Factor (HIF) complexes with the coactivator p300, activating the hypoxia response pathway and allowing tumors to grow. The CH1 and CAD domains of each respective protein form the interface between p300 and ...

Murine xenograft model

Author: FengZhi Liu, date: 06/05/2012, view: 18519, Q&A: 1

This experiment is used to test the efficacies of chemo treatments or gene therapy in an in vivo system. In this protocol, the mouse xenograft model is used.

Protocol for knockdown of HuR with siRNA

Author: FengZhi Liu, date: 05/20/2012, view: 10472, Q&A: 1

Through a base-pairing-dependent mechanism, 21-23 nucleotide (nt) siRNA binds to complementary target mRNA to inhibit translation.

Cell Proliferation Assay by Flow Cytometry (BrdU and PI Staining)

Author: Hui Zhu, date: 04/05/2012, view: 60002, Q&A: 2

Cell Proliferation assays include an important set of fluorescence-based tests that can monitor cell health and cell division by evaluating DNA synthesis through thymidine incorporation. Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a ...

Protein Extraction from Mice Xenograft Tumor

Author: Huan Pang, date: 04/05/2012, view: 21003, Q&A: 1

Proteins from frozen tissue such as xenograft tumor need to be extracted efficiently without being degraded to make the best use of a limited resource and to ensure that an accurate representation of proteins in selected tumor tissue can be ...

Protocol to Determine Mitotic Index by FACS

Author: Hui Zhu, date: 03/20/2012, view: 16562, Q&A: 0

Fluorescence Activated Cell Sorting (FACS) is a sensitive method to count mitotic cells. Cells are stained with an antibody that recognizes an antigen present only in mitotic cells, combined with propidium iodide (PI) to stain DNA. Two-dimensional ...

Find out more

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.