DE
David R Edgell
  • Professor and Acting Chair, Department of Biochemistry, Department of Biochemistry, Schulich School of Medicine & Dentistry, The University of Western Ontario, Canada, Canada,
Research fields
  • Microbiology
Personal information

Education

PhD, Dalhousie

Research focus

Genome editing, synthetic biology, mobile genetic elements

Publications

Pereira CV, Bacman SR, Arguello T, Zekonyte U, Williams SL, Edgell DR, Moraes
CT. mitoTev-TALE: a monomeric DNA editing enzyme to reduce mutant mitochondrial
DNA levels. EMBO Mol Med. 2018 Jul 16. pii: e8084. doi: 10.15252/emmm.201708084.
[Epub ahead of print] PubMed PMID: 30012581.


Kaiser JC, King AN, Grigg JC, Sheldon JR, Edgell DR, Murphy MEP, Brinsmade SR,
Heinrichs DE. Repression of branched-chain amino acid synthesis in Staphylococcus
aureus is mediated by isoleucine via CodY, and by a leucine-rich attenuator
peptide. PLoS Genet. 2018 Jan 22;14(1):e1007159. doi:
10.1371/journal.pgen.1007159. eCollection 2018 Jan. PubMed PMID: 29357354; PubMed
Central PMCID: PMC5794164.


Slattery SS, Diamond A, Wang H, Therrien JA, Lant JT, Jazey T, Lee K, Klassen
Z, Desgagné-Penix I, Karas BJ, Edgell DR. An Expanded Plasmid-Based Genetic
Toolbox Enables Cas9 Genome Editing and Stable Maintenance of Synthetic Pathways
in Phaeodactylum tricornutum. ACS Synth Biol. 2018 Feb 16;7(2):328-338. doi:
10.1021/acssynbio.7b00191. Epub 2018 Jan 8. PubMed PMID: 29298053.


Guha TK, Edgell DR. Applications of Alternative Nucleases in the Age of
CRISPR/Cas9. Int J Mol Sci. 2017 Nov 29;18(12). pii: E2565. doi:
10.3390/ijms18122565. Review. PubMed PMID: 29186020; PubMed Central PMCID:
PMC5751168.


Wolfs JM, Hamilton TA, Lant JT, Laforet M, Zhang J, Salemi LM, Gloor GB,
Schild-Poulter C, Edgell DR. Biasing genome-editing events toward precise length
deletions with an RNA-guided TevCas9 dual nuclease. Proc Natl Acad Sci U S A.
2016 Dec 27;113(52):14988-14993. doi: 10.1073/pnas.1616343114. Epub 2016 Dec 12.
PubMed PMID: 27956611; PubMed Central PMCID: PMC5206545.


Roy AC, Wilson GG, Edgell DR. Perpetuating the homing endonuclease life cycle:
identification of mutations that modulate and change I-TevI cleavage preference.
Nucleic Acids Res. 2016 Sep 6;44(15):7350-9. doi: 10.1093/nar/gkw614. Epub 2016
Jul 7. PubMed PMID: 27387281; PubMed Central PMCID: PMC5009752.


Wolfs JM, DaSilva M, Meister SE, Wang X, Schild-Poulter C, Edgell DR.
MegaTevs: single-chain dual nucleases for efficient gene disruption. Nucleic
Acids Res. 2014 Jul;42(13):8816-29. doi: 10.1093/nar/gku573. Epub 2014 Jul 10.
PubMed PMID: 25013171; PubMed Central PMCID: PMC4117789.


McMurrough TA, Dickson RJ, Thibert SM, Gloor GB, Edgell DR. Control of
catalytic efficiency by a coevolving network of catalytic and noncatalytic
residues. Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):E2376-83. doi:
10.1073/pnas.1322352111. Epub 2014 May 27. PubMed PMID: 24912189; PubMed Central
PMCID: PMC4060692.


Kleinstiver BP, Wang L, Wolfs JM, Kolaczyk T, McDowell B, Wang X,
Schild-Poulter C, Bogdanove AJ, Edgell DR. The I-TevI nuclease and linker domains
contribute to the specificity of monomeric TALENs. G3 (Bethesda). 2014 Apr
16;4(6):1155-65. doi: 10.1534/g3.114.011445. PubMed PMID: 24739648; PubMed
Central PMCID: PMC4065259.


Kleinstiver BP, Wolfs JM, Edgell DR. The monomeric GIY-YIG homing
endonuclease I-BmoI uses a molecular anchor and a flexible tether to sequentially
nick DNA. Nucleic Acids Res. 2013 May 1;41(10):5413-27. doi: 10.1093/nar/gkt186.
Epub 2013 Apr 4. PubMed PMID: 23558745; PubMed Central PMCID: PMC3664794.


Kleinstiver BP, Wolfs JM, Kolaczyk T, Roberts AK, Hu SX, Edgell DR. Monomeric
site-specific nucleases for genome editing. Proc Natl Acad Sci U S A. 2012 May
22;109(21):8061-6. doi: 10.1073/pnas.1117984109. Epub 2012 May 7. PubMed PMID:
22566637; PubMed Central PMCID: PMC3361397.
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