Samuel Wilson Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA
3 protocols

Julie Horton Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA
1 protocol
Alexandros Alexandratos National and Kapodistrian University of Athens
39 protocols

Chong He Buck Institute for Research on Aging
7 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
95 protocols

Gregory Finnigan Kansas State University
2 protocols

Melike Çağlayan
  • Assist. Prof., University of Florida
Research focus
  • Biochemistry
  • 3 Author merit



Lab information

Caglayan Lab

1) Caglayan M. and Wilson S.H. Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals a promutagenic DNA intermediate during double strand break repair. Nature Communications (2018) 9: 4213.
2) Horton J.K., Stefanick D.F., Caglayan M., Zhao M.L., Gassman N.R., Wilson S.H. XRCC1 phosphorylation affects aprataxin recruitment and DNA deadenylation activity. DNA Repair (2018) – 64: 26 – 33.
3) Prasad R., Caglayan M., Da-Peng D., Nadalutti C.A., Gassman N.R., Zhao M., Stefanick D.F., Horton J.K., Krasich R., Longley M.J., Copeland W.C., Griffith J.D., Wilson S.H. DNA polymerase β: The missing link of the base excision repair machinery in mammalian mitochondria. DNA Repair (2017) 60: 77 – 88.
4) Caglayan M., Prasad R., Krasich R., Longley M.J., Kadoda K., Tsuda M., Sasanuma H., Takeda S., Tano K., Copeland W.C., Wilson S.H. Complementation of aprataxin deficiency by base excision repair enzymes in mitochondrial extracts. Nucleic Acids Research (2017) 17: 10079 – 10088.
5) Caglayan M., Wilson S.H. Role of DNA polymerase β oxidized nucleotide insertion in DNA ligation failure. Journal of Radiation Research - Review article (2017) 1093: 1 – 5.
6) Caglayan M., Horton J.K., Da-Peng D., Stefanick D.F., Wilson S.H. Oxidized nucleotide insertion by pol β confounds ligation during base excision repair. Nature Communications (2017) 8: 14045.
7) Sassa A., Caglayan M., Rodriguez Y., Beard W.A., Wilson S.H., Nohmi T., Honma M., Yasui M. Impact of ribonucleotide backbone on translesion synthesis and repair of 7,8-Dihydro-8-oxoguanine. Journal of Biological Chemistry (2016) 291: 24314 – 24323.
8) Caglayan M., Wilson S.H. Oxidant and environmental toxicant-induced effects compromise DNA ligation during base excision DNA repair. DNA Repair - Review article (2015) 35: 85 – 89.
9) Caglayan M., Horton J.K., Prasad R., Wilson S.H. Complementation of aprataxin deficiency by base excision repair enzymes. Nucleic Acids Research (2015) 43: 2271 – 2281.
10) Caglayan M., Batra V.K., Sassa A., Prasad R., Wilson S.H. Role of polymerase β in complementing aprataxin deficiency during abasic-site base excision repair. Nature Structural and Molecular Biology (2014) 21: 497 – 499.
11) Sassa A., Caglayan M., Dyrkheeva N.S., Beard W.A., Wilson S.H. Base excision repair of tandem modifications in a methylated CpG dinucleotide. Journal of Biological Chemistry (2014) 289: 13996 – 4008.
3 Protocols published
In vitro Assay to Measure DNA Polymerase β Nucleotide Insertion Coupled with the DNA Ligation Reaction during Base Excision Repair
Authors:  Melike Çağlayan and Samuel H. Wilson, date: 06/20/2017, view: 5578, Q&A: 0
We previously reported that oxidized nucleotide insertion by DNA polymerase β (pol β) can confound the DNA ligation step during base excision repair (BER) (Çağlayan et al., 2017). Here, we describe a method to investigate pol β nucleotide ...
Enzymatic Activity Assays for Base Excision Repair Enzymes in Cell Extracts from Vertebrate Cells
Authors:  Melike Çağlayan, Julie K. Horton and Samuel H. Wilson, date: 06/05/2015, view: 6493, Q&A: 0
We previously reported enzymatic activity assays for the base excision repair (BER) enzymes DNA polymerase β (pol β), aprataxin (APTX), and flap endonuclease 1 (FEN1) in cell extracts from Saccharomyces cerevisiae (Çağlayan and Wilson, ...
4 Protocols reviewed
Purification and HPLC Analysis of Cell Wall Muropeptides from Caulobacter crescentus
Authors:  Gabriele Stankeviciute and Eric A. Klein, date: 11/05/2019, view: 1924, Q&A: 0
The peptidoglycan sacculus, or cell wall, is what defines bacterial cell shape. Cell wall composition can be best characterized at the molecular level by digesting the peptidoglycan murein polymer into its muropeptide subunits and quantifying the ...
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RNA Capping by Transcription Initiation with Non-canonical Initiating Nucleotides (NCINs): Determination of Relative Efficiencies of Transcription Initiation with NCINs and NTPs
Authors:  Jeremy G. Bird, Bryce E. Nickels and Richard H. Ebright, date: 06/20/2017, view: 6010, Q&A: 0
It recently has been established that adenine-containing cofactors, including nicotinamide adenine dinucleotide (NAD+), reduced nicotinamide adenine dinucleotide (NADH), and 3’-desphospho-coenzyme A (dpCoA), can serve as ‘non-canonical ...
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