Jason Reuter —BIO-PROTOCOL

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Jason Reuter

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4 Protocols published

Subcutaneous Injection of Tumor Cells

Author: Jason Reuter, date: 12/20/2011, view: 45199, Q&A: 2

Growth of cells in the subcutaneous space of immunocompromised mice is a common method for assaying tumorigenic potential in vivo. This technique is also used to assess the effects of therapeutic interventions on cancer cell lines.

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ngu trinh

trinh van ngu

why need to use Matrigel in this experiment? what is the function of Matrigel? Any research did without Matrigel?

12/10/2018 5:43:40 PM Reply

ngu trinh

trinh van ngu

Thank you so much for your response.

2018-12-14 18:17:25

shohreh fakhari

kurdistan university of medical sciences

Matrigel has been shown to significantly enhance the tumorigenicity of a wide range of cancer cell lines in vivo.
https://link.springer.com/protocol/10.1385/1-59259-406-9:287
if you don't want to use matrigel, you should use large number of cells for increased tumorigenicity of cancer cells.

2018-12-11 03:00:33

Isolation of Primary Human Keratinocytes from Foreskin

Author: Jason Reuter, date: 12/20/2011, view: 12868, Q&A: 0

Keratinocytes are the primary constituents of human skin, the functional barrier between our bodies and the external environment. The balance between keratinocyte differentiation and self-renewal is crucial to skin homeostasis. Primary keratinocyte ...

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