Jason Reuter —BIO-PROTOCOL

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Jason Reuter

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4 Protocols published

Subcutaneous Injection of Tumor Cells

Author: Jason Reuter, date: 12/20/2011, view: 34169, Q&A: 1

Growth of cells in the subcutaneous space of immunocompromised mice is a common method for assaying tumorigenic potential in vivo. This technique is also used to assess the effects of therapeutic interventions on cancer cell lines.

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emilia

What can happen if you inject the tumor cells in complete medium? Will this affect the experiment?
Thank you!

7/10/2014 11:36:48 PM Reply

emilia

ok. Thank you very much for your advice and quick reply.

2014-07-11 00:35:30

Jason Reuter

The fluid will be absorbed. However, it may support the cells initially. My only point is that you haven't strictly tested the cells' ability to grow tumors independent of additional supplements.

2014-07-11 00:28:01

emilia

In which way may the tumor growth depend on the serum? Will not it be drain out if it is in small volum(100ul)? Thx

2014-07-10 23:59:23

Jason Reuter

You should be consistent between, but I expect the experiment will work. It should also be acknowledged that tumor growth may depend on serum and medium.

2014-07-10 23:49:13

Isolation of Primary Human Keratinocytes from Foreskin

Author: Jason Reuter, date: 12/20/2011, view: 10050, Q&A: 0

Keratinocytes are the primary constituents of human skin, the functional barrier between our bodies and the external environment. The balance between keratinocyte differentiation and self-renewal is crucial to skin homeostasis. Primary keratinocyte ...

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