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Coauthors
Margarita Salas Centro de Biología Molecular Severo Ochoa, Centro de Biología Molecular Severo Ochoa, Spain, Spain,
1 protocol

Mónica Berjón-Otero Present address: Max Planck Institute for Medical Research, Germany, Germany,
1 protocol

Ana Lechuga Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones científicas y Universidad Autónoma de Madrid, Spain, Spain,
1 protocol

María Salas Centro de Biología Molecular Severo Ochoa, CSIC/UAM, Spain
1 protocol
Coreviewers
Alba Blesa Universidad Francisco de Vitoria
3 protocols

Arsalan Daudi University of California
154 protocols

Chao Jiang Stanford University
3 protocols

Claudia Catalanotti 10x Genomics
14 protocols

Editor
Modesto Redrejo-Rodriguez
  • Research associate, Centro de Biología Molecular Severo Ochoa, Spain
Research focus
  • Molecular biology
  • DNA Replication and Repair mechanisms of viruses and mobile elements
  • 2 Author merit

Education

PhD, Universidad Autónoma de Madrid (Spain), 2009

Publications

https://www.ncbi.nlm.nih.gov/sites/myncbi/modesto.redrejo%20rodriguez.1/bibliography/41957182/public/?sort=date&direction=descending

2 Protocols published
Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
Authors:  Ana Lechuga, Mónica Berjón-Otero, Margarita Salas and Modesto Redrejo-Rodríguez, date: 01/05/2018, view: 443, Q&A: 0
This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation. One of the proteins is overexpressed in E. coli as HA-tagged recombinant protein and cell-free extracts are ...
Determination of Mutation Frequency During Viral DNA Replication
This protocol is a simple method for evaluating mutation frequency during African swine fever virus (ASFV) replication, although it could be used also for other DNA viruses (poxvirus, herpesvirus, mimivirus, etc) with minor modifications. ...
9 Protocols reviewed
Quantification of Densities of Bacterial Endosymbionts of Insects by Real-time PCR
Author:  Daisuke Kageyama, date: 10/05/2017, view: 951, Q&A: 0
Increased attention has been paid to the endosymbiotic bacteria of insects. Because most insect endosymbionts are uncultivable, quantitative PCR (qPCR) is a practical and convenient method to quantify endosymbiont titers. Here we report a protocol ...
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Escherichia coli Infection of Drosophila
Authors:  Charles Tracy and Helmut Krämer, date: 05/05/2017, view: 1747, Q&A: 0
Following septic insults, healthy insects, just like vertebrates, mount a complex immune response to contain and destroy pathogens. The failure to efficiently clear bacterial infections in immuno-compromised fly mutants leads to higher mortality ...
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8 Protocols edited
Targeted Genome Editing of Virulent Phages Using CRISPR-Cas9
Authors:  Marie-Laurence Lemay, Ariane C. Renaud, Geneviève M. Rousseau and Sylvain Moineau, date: 01/05/2018, view: 594, Q&A: 0
This protocol describes a straightforward method to generate specific mutations in the genome of strictly lytic phages. Briefly, a targeting CRISPR-Cas9 system and a repair template suited for homologous recombination are provided inside a bacterial ...
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Rolling Circle Amplification to Screen Yam Germplasm for Badnavirus Infections and to Amplify and Characterise Novel Badnavirus Genomes
Since the first discovery of badnaviruses (family Caulimoviridae, genus Badnavirus) in yam (Dioscorea spp.) germplasm in the 1970s (Harrison and Roberts, 1973), several hundred partial badnavirus reverse transcriptase ...
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