Margarita Salas Centro de Biología Molecular Severo Ochoa, Centro de Biología Molecular Severo Ochoa, Spain, Spain,
1 protocol

Mónica Berjón-Otero Present address: Max Planck Institute for Medical Research, Germany, Germany,
1 protocol

Ana Lechuga Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones científicas y Universidad Autónoma de Madrid, Spain, Spain,
1 protocol

María Salas Centro de Biología Molecular Severo Ochoa, CSIC/UAM, Spain
1 protocol
Adison Wong Singapore Institute of Technology
1 protocol

Alba Blesa Universidad Francisco de Vitoria
23 protocols

Anna Köhler Georg August Universität Göttingen
1 protocol

Arsalan Daudi University of California
157 protocols

Editor
Modesto Redrejo-Rodriguez
  • Assistant Professor, Biochemistry Department, Madrid Autonomous University
Research focus
  • Molecular biology
  • DNA Replication and Repair mechanisms of viruses and mobile elements
  • 2 Author merit
2 Protocols published
Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
Authors:  Ana Lechuga, Mónica Berjón-Otero, Margarita Salas and Modesto Redrejo-Rodríguez, date: 01/05/2018, view: 6511, Q&A: 0
This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation. One of the proteins is overexpressed in E. coli as HA-tagged recombinant protein and cell-free extracts are ...
Determination of Mutation Frequency During Viral DNA Replication
This protocol is a simple method for evaluating mutation frequency during African swine fever virus (ASFV) replication, although it could be used also for other DNA viruses (poxvirus, herpesvirus, mimivirus, etc) with minor modifications. ...
10 Protocols reviewed
Giant Mimiviridae CsCl Purification Protocol
Authors:  Lionel Bertaux, Audrey Lartigue and Sandra Jeudy, date: 11/20/2020, view: 615, Q&A: 0
While different giant viruses’ purification protocols are available, they are not fully described and they use sucrose gradient that does not reach an equilibrium. Here, we report a protocol for the purification of members of the Mimiviridae ...
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Quantification of Densities of Bacterial Endosymbionts of Insects by Real-time PCR
Author:  Daisuke Kageyama, date: 10/05/2017, view: 7247, Q&A: 0
Increased attention has been paid to the endosymbiotic bacteria of insects. Because most insect endosymbionts are uncultivable, quantitative PCR (qPCR) is a practical and convenient method to quantify endosymbiont titers. Here we report a protocol ...
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20 Protocols edited
Protein- and Sequencing-based Massively Parallel Single-cell Approaches to Gene Expression Profiling
Authors:  Xuhuai Ji, Igor Goncharov, Miao Zhao, Molly Miranda and Holden T. Maecker, date: 02/05/2019, view: 4882, Q&A: 0
Understanding cellular diversity and dynamics in immune cells are critical for elucidating mechanisms of diagnosis, response to therapeutics, and patient prognosis. Our goal is to apply a technically simple approach for gene expression cytometry ...
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Quantitative Transformation Efficiency Assay for Bacillus subtilis
Authors:  Christian L. Loyo and Briana M. Burton, date: 12/05/2018, view: 4487, Q&A: 0
Bacillus subtilis (B. subtilis) is a model Gram-positive organism used to study a variety of physiological states and stress responses, one of which is the development of competence. Competence refers to the physiological state of ...
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