Margarita Salas Centro de Biología Molecular Severo Ochoa, Centro de Biología Molecular Severo Ochoa, Spain, Spain,
1 protocol

Mónica Berjón-Otero Present address: Max Planck Institute for Medical Research, Germany, Germany,
1 protocol

Ana Lechuga Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones científicas y Universidad Autónoma de Madrid, Spain, Spain,
1 protocol

María Salas Centro de Biología Molecular Severo Ochoa, CSIC/UAM, Spain
1 protocol
Adison Wong Singapore Institute of Technology
1 protocol

Alba Blesa Universidad Francisco de Vitoria
4 protocols

Arsalan Daudi University of California
157 protocols

Benoit Chassaing Georgia State University
14 protocols

Editor
Modesto Redrejo-Rodriguez
  • Assistant Professor, Biochemistry Department, Madrid Autonomous University
Research focus
  • Molecular biology
  • DNA Replication and Repair mechanisms of viruses and mobile elements
  • 2 Author merit
2 Protocols published
Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
Authors:  Ana Lechuga, Mónica Berjón-Otero, Margarita Salas and Modesto Redrejo-Rodríguez, date: 01/05/2018, view: 1999, Q&A: 0
This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation. One of the proteins is overexpressed in E. coli as HA-tagged recombinant protein and cell-free extracts are ...
Determination of Mutation Frequency During Viral DNA Replication
This protocol is a simple method for evaluating mutation frequency during African swine fever virus (ASFV) replication, although it could be used also for other DNA viruses (poxvirus, herpesvirus, mimivirus, etc) with minor modifications. ...
9 Protocols reviewed
Rust Removal Experiments
Authors:  Mahmoud Kamal Ahmadi and Blaine A. Pfeifer, date: 04/05/2016, view: 6732, Q&A: 0
Iron oxidation, known as rust formation, causes enormous loss in term of property damages and associated economic risks. Depending on the degree of formation, rust consists of several layers of iron in different oxidation states. The brownish top ...
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RNase H Polymerase-independent Cleavage Assay for Evaluation of RNase H Activity of Reverse Transcriptase Enzymes
Authors:  Angela Corona and Enzo Tramontano, date: 08/20/2015, view: 5320, Q&A: 0
The ribonuclease H (RNase H) polymerase-independent cleavage assay allows detection and quantification of RNase H activity of reverse transcriptase (RT) enzymes with a hybrid substrate formed by a fluorescein labeled RNA annealed with Dabcyl DNA ...
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18 Protocols edited
Plant Assays for Quantifying Ralstonia solanacearum Virulence
Authors:  Devanshi Khokhani, Tuan Minh Tran , Tiffany M. Lowe-Power and Caitilyn Allen, date: 09/20/2018, view: 416, Q&A: 0
Virulence assays are powerful tools to study microbial pathogenesis in vivo. Good assays track disease development and, coupled with targeted mutagenesis, can identify pathogen virulence factors. Disease development in plants is extremely ...
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CRISPR/Cas Gene Editing of a Large DNA Virus: African Swine Fever Virus
Gene editing of large DNA viruses, such as African swine fever virus (ASFV), has traditionally relied on homologous recombination of a donor plasmid consisting of a reporter cassette with surrounding homologous viral DNA. However, this homologous ...
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