Alka Mehra Institute of Genomics and Integrative Biology
20 protocols

David Cisneros Umeå University
23 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
42 protocols

Reviewer
Ran Chen
  • Stanford University
Research focus
  • Molecular biology
  • 7 Author merit
7 Protocols published
Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’)
Author:  Ran Chen, date: 02/05/2012, view: 45874, Q&A: 13
Bone marrow derived macrophages are a type of white blood cell that can be isolated from mammalian bone marrow. In this protocol, a method is described in which bone marrow cells are isolated from mouse leg bones (femur and tibia), and then ...
MTT Assay of Cell Numbers after Drug/Toxin Treatment
Author:  Ran Chen, date: 04/05/2011, view: 17429, Q&A: 1
MTT assay is a colorimetric method for measuring the activity of enzymes in living cells that reduce MTT to formazan dyes, giving a purple color. It is commonly used to determine cytotoxicity of potential medicinal agents and toxic materials, since ...
2 Protocols reviewed
Delivery of the Cas9 or TevCas9 System into Phaeodactylum tricornutum via Conjugation of Plasmids from a Bacterial Donor
Authors:  Helen Wang, Samuel S. Slattery, Bogumil J. Karas and David R. Edgell, date: 08/20/2018, view: 719, Q&A: 0
Diatoms are an ecologically important group of eukaryotic microalgae with properties that make them attractive for biotechnological applications such as biofuels, foods, cosmetics and pharmaceuticals. Phaeodactylum tricornutum is a model ...
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Ectopic Gene Expression in Macrophages Using in vitro Transcribed mRNA
Authors:  Pallavi Chandra and Jennifer A. Philips, date: 05/20/2018, view: 1128, Q&A: 0
Macrophages are immune cells that contribute to host defense through various mechanisms including phagocytosis and antigen presentation. Their antimicrobial capacity is subverted by clinically important intracellular pathogens such as Mycobacterium ...
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