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A peer-reviewed protocol journal. No publication fee; no access fee.
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Alka Mehra
Institute of Genomics and Integrative Biology
38 protocols
Chhuttan Meena
National Chemical laboratory NCL Pune
2 protocols
David Cisneros
Umeå University
33 protocols
Gal Haimovich
Molecular Genetics, Weizmann Institute of Science, Israel
95 protocols
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Reviewer
Ran Chen
CEO, Zhejiang JFK Biological Technology Co. Ltd.
Research focus
Molecular biology
Contributions
7
Author merit
Reviewer merit
Editor merit
7 Protocols published
Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’)
Author:
Ran Chen
,
date:
02/05/2012,
view:
60205,
Q&A:
15
Bone marrow derived macrophages are a type of white blood cell that can be isolated from mammalian bone marrow. In this protocol, a method is described in which bone marrow cells are isolated from mouse leg bones (femur and tibia), and then ...
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fang xiao
zhengzhou university
Hello！ M-CSF's volume is 40ng/ml, what effect will it have?
2/25/2019 9:40:59 AM
Reply
MTT Assay of Cell Numbers after Drug/Toxin Treatment
Author:
Ran Chen
,
date:
04/05/2011,
view:
22471,
Q&A:
1
MTT assay is a colorimetric method for measuring the activity of enzymes in living cells that reduce MTT to formazan dyes, giving a purple color. It is commonly used to determine cytotoxicity of potential medicinal agents and toxic materials, since ...
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Yuanqing Lin
How many number of cells should be placed in each well for effective assay?
7/20/2011 5:14:19 PM
Reply
bio-protocol
It depends on the cell size, growth rate and treatment time. Usually we let the cells reach 50%-80% confluence before the treatment if the treatment time is within 1 day.
2011-08-31 16:03:58
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3 Protocols reviewed
Evaluation of the Sequence Variability within the PCR Primer/Probe Target Regions of the SARS-CoV-2 Genome
Authors:
Kashif Aziz Khan
and
Peter Cheung
,
date:
12/20/2020,
view:
902,
Q&A:
0
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; initially named 2019-nCoV) is responsible for the recent coronavirus disease (COVID-19) pandemic, and polymerase chain reaction (PCR) is the current standard method for diagnosis from ...
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Delivery of the Cas9 or TevCas9 System into
Phaeodactylum tricornutum
via Conjugation of Plasmids from a Bacterial Donor
Authors:
Helen Wang
,
Samuel S. Slattery
,
Bogumil J. Karas
and
David R. Edgell
,
date:
08/20/2018,
view:
4348,
Q&A:
2
Diatoms are an ecologically important group of eukaryotic microalgae with properties that make them attractive for biotechnological applications such as biofuels, foods, cosmetics and pharmaceuticals.
Phaeodactylum tricornutum
is a model ...
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