Alessandro Didonna University of California, San Francisco
47 protocols

Alka Mehra Institute of Genomics and Integrative Biology
39 protocols

Chhuttan Meena National Chemical laboratory NCL Pune
2 protocols

David Cisneros Umeå University
33 protocols

Reviewer
Ran Chen
  • CEO, Zhejiang JFK Biological Technology Co. Ltd.
Research focus
  • Molecular biology
  • 7 Author merit
7 Protocols published
Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’)
Author:  Ran Chen, date: 02/05/2012, view: 61946, Q&A: 15
Bone marrow derived macrophages are a type of white blood cell that can be isolated from mammalian bone marrow. In this protocol, a method is described in which bone marrow cells are isolated from mouse leg bones (femur and tibia), and then ...
MTT Assay of Cell Numbers after Drug/Toxin Treatment
Author:  Ran Chen, date: 04/05/2011, view: 23044, Q&A: 1
MTT assay is a colorimetric method for measuring the activity of enzymes in living cells that reduce MTT to formazan dyes, giving a purple color. It is commonly used to determine cytotoxicity of potential medicinal agents and toxic materials, since ...
4 Protocols reviewed
COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR

The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and

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Evaluation of the Sequence Variability within the PCR Primer/Probe Target Regions of the SARS-CoV-2 Genome
Authors:  Kashif Aziz Khan and Peter Cheung, date: 12/20/2020, view: 1568, Q&A: 0
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; initially named 2019-nCoV) is responsible for the recent coronavirus disease (COVID-19) pandemic, and polymerase chain reaction (PCR) is the current standard method for diagnosis from ...
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