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A peer-reviewed protocol journal. No publication fee; no access fee.
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Reviewed
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Q&A
Alka Mehra
Institute of Genomics and Integrative Biology
36 protocols
David Cisneros
Umeå University
30 protocols
Gal Haimovich
Molecular Genetics, Weizmann Institute of Science, Israel
93 protocols
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Reviewer
Ran Chen
CEO, Zhejiang JFK Biological Technology Co. Ltd.
Research focus
Molecular biology
Contributions
7
Author merit
Reviewer merit
Editor merit
7 Protocols published
Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’)
Author:
Ran Chen
,
date:
02/05/2012,
view:
57068,
Q&A:
15
Bone marrow derived macrophages are a type of white blood cell that can be isolated from mammalian bone marrow. In this protocol, a method is described in which bone marrow cells are isolated from mouse leg bones (femur and tibia), and then ...
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fang xiao
zhengzhou university
Hello！ M-CSF's volume is 40ng/ml, what effect will it have?
2/25/2019 9:40:59 AM
Reply
MTT Assay of Cell Numbers after Drug/Toxin Treatment
Author:
Ran Chen
,
date:
04/05/2011,
view:
21528,
Q&A:
1
MTT assay is a colorimetric method for measuring the activity of enzymes in living cells that reduce MTT to formazan dyes, giving a purple color. It is commonly used to determine cytotoxicity of potential medicinal agents and toxic materials, since ...
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Yuanqing Lin
How many number of cells should be placed in each well for effective assay?
7/20/2011 5:14:19 PM
Reply
bio-protocol
It depends on the cell size, growth rate and treatment time. Usually we let the cells reach 50%-80% confluence before the treatment if the treatment time is within 1 day.
2011-08-31 16:03:58
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2 Protocols reviewed
Delivery of the Cas9 or TevCas9 System into
Phaeodactylum tricornutum
via Conjugation of Plasmids from a Bacterial Donor
Authors:
Helen Wang
,
Samuel S. Slattery
,
Bogumil J. Karas
and
David R. Edgell
,
date:
08/20/2018,
view:
3478,
Q&A:
2
Diatoms are an ecologically important group of eukaryotic microalgae with properties that make them attractive for biotechnological applications such as biofuels, foods, cosmetics and pharmaceuticals.
Phaeodactylum tricornutum
is a model ...
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Ectopic Gene Expression in Macrophages Using
in vitro
Transcribed mRNA
Authors:
Pallavi Chandra
and
Jennifer A. Philips
,
date:
05/20/2018,
view:
3849,
Q&A:
0
Macrophages are immune cells that contribute to host defense through various mechanisms including phagocytosis and antigen presentation. Their antimicrobial capacity is subverted by clinically important intracellular pathogens such as
Mycobacterium
...
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