Macrophages usually attach to the plastic firmly while other cells, e.g. fibroblast cells, loosely attach. Thereby, other cells can be washed out by using PBS during the culture and after about 10 days, almost all cells become attached macrophages. You can also use Trypsin-EDTA to facilitate the process... Macrophages can still remain attached to the plastic after 1min treatment with Trypsin-EDTA while other cells become completely detached ... 2016-03-06 23:28:01

It depends on the cell size, growth rate and treatment time. Usually we let the cells reach 50%-80% confluence before the treatment if the treatment time is within 1 day. 2011-08-31 16:03:58