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Jeffrey Gerst Molecular Genetics, Weizmann Institute of Science, Israel, Israel,
1 protocol
Abhijit Kale Deciduous Therapeutics
 10 protocols

Akira Karasawa New York Structural Biology Center
 8 protocols

Alexandros Kokotos Weill Cornell Medicine
 18 protocols

Alka Mehra Institute of Genomics and Integrative Biology
 40 protocols

Editor
Gal Haimovich
  • Research scientist, Molecular Genetics, Weizmann Institute of Science, Israel
Research focus
  • Cell biology
  • mRNA localization, mRNA transfer, membrane nanotubes, exosomes
  • 2 Author merit
Lab Protocol Hub

Education

PhD, Technion - Israeli institute of technology, 2011

Lab information

Jeffrey Gerst lab http://www.weizmann.ac.il/molgen/Gerst/
We are studying protein and mRNA localization in eukaryotic cells (mainly yeast & mammalian cells).
http://www.weizmann.ac.il/molgen/Gerst/

Publications

  1. https://greenfluorescentblog.wordpress.com/about/list-of-publications/
  2. Haimovich, G., Zabezhinsky, D., Haas, B., Slobodin, B., Purushothaman, P., Fan, L., Levin, J. Z., Nusbaum, C. and Gerst, J. E. (2016). Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to "MS2 coat proteins bound to yeast mRNAs block 5' to 3' degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system". RNA 22(5): 660-666.
  3. Haimovich, G., Cohen-Zontag, O. and Gerst, J. E. (2016). A role for mRNA trafficking and localized translation in peroxisome biogenesis and function? Biochim Biophys Acta 1863(5): 911-921.
  4. Buxbaum, A. R.*, Haimovich, G.* and Singer, R. H. (2015). In the right place at the right time: visualizing and understanding mRNA localization. Nat Rev Mol Cell Biol 16(2): 95-109. (*Equal contribution)
  5. Somekh, J., Haimovich, G., Guterman, A., Dori, D. and Choder, M. (2014). Conceptual modeling of mRNA decay provokes new hypotheses. PLoS One 9(9): e107085.
  6. Haimovich, G., Medina, D. A., Causse, S. Z., Garber, M., Millan-Zambrano, G., Barkai, O., Chavez, S., Perez-Ortin, J. E., Darzacq, X. and Choder, M. (2013). Gene expression is circular: factors for mRNA degradation also foster mRNA synthesis. Cell 153(5): 1000-1011.
  7. Haimovich, G., Choder, M., Singer, R. H. and Trcek, T. (2013). The fate of the messenger is pre-determined: a new model for regulation of gene expression. Biochim Biophys Acta 1829(6-7): 643-653.
  8. Harel-Sharvit, L., Eldad, N., Haimovich, G., Barkai, O., Duek, L. and Choder, M. (2010). RNA polymerase II subunits link transcription and mRNA decay to translation. Cell 143(4): 552-563.
  9. Goler-Baron, V., Selitrennik, M., Barkai, O., Haimovich, G., Lotan, R. and Choder, M. (2008). Transcription in the nucleus and mRNA decay in the cytoplasm are coupled processes. Genes Dev 22(15): 2022-2027.
  10. Lotan, R., Goler-Baron, V., Duek, L., Haimovich, G. and Choder, M. (2007). The Rpb7p subunit of yeast RNA polymerase II plays roles in the two major cytoplasmic mRNA decay mechanisms. J Cell Biol 178(7): 1133-1143.
  11. Kamer, I., Sarig, R., Zaltsman, Y., Niv, H., Oberkovitz, G., Regev, L., Haimovich, G., Lerenthal, Y., Marcellus, R. C. and Gross, A. (2005). Proapoptotic BID is an ATM effector in the DNA-damage response. Cell 122(4): 593-603.
  12. Weiss, M., Haimovich, G. and Pick, U. (2001). Phosphate and Sulfate uptake in the halotolerant alga Dunaliella are driven by Na+-symport mechanism. J Plant Physiology 158:1519-1525.



2 Protocols published
Single-molecule Fluorescence in situ Hybridization (smFISH) for RNA Detection in Adherent Animal Cells
Authors:  Gal Haimovich and Jeffrey E. Gerst, date: 11/05/2018, view: 23759, Q&A: 1
Transcription and RNA decay play critical roles in the process of gene expression and the ability to accurately measure cellular mRNA levels is essential for understanding this regulation. Here, we describe a single-molecule fluorescent in situ ...
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Plasmid Extract from Budding Yeast (Saccharomyces cerevisiae)
Author:  Gal Haimovich, date: 07/20/2018, view: 7577, Q&A: 0
Plasmids are widely used tools in yeast research. In many cases, plasmid libraries are used in genetic screens or in yeast two hybrid screens. In such cases, it is necessary to extract plasmids carrying unknown genetic elements from positive clones ...
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4 Protocols reviewed
Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
Authors:  Rachna Narayanan and Andrew C. Oates, date: 03/20/2019, view: 9686, Q&A: 0
In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes ...
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Ectopic Gene Expression in Macrophages Using in vitro Transcribed mRNA
Authors:  Pallavi Chandra and Jennifer A. Philips, date: 05/20/2018, view: 4859, Q&A: 0
Macrophages are immune cells that contribute to host defense through various mechanisms including phagocytosis and antigen presentation. Their antimicrobial capacity is subverted by clinically important intracellular pathogens such as Mycobacterium ...
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94 Protocols edited
Dual Color, Live Imaging of Vesicular Transport in Axons of Cultured Sensory Neurons
Authors:  Yoko Bekku and James L. Salzer, date: 06/20/2021, view: 846, Q&A: 0

The function of neurons in afferent reception, integration, and generation of electrical activity relies on their strikingly polarized organization, characterized by distinct membrane domains. These domains have different compositions resulting from

...
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Quantitation of Secretory Granule Size in Drosophila Larval Salivary Glands
Authors:  Cheng-I J. Ma and Julie A. Brill, date: 06/05/2021, view: 1116, Q&A: 0

Maturation of secretory granules is a crucial process that ensures the bioactivity of cargo proteins undergoing regulated secretion. In Drosophila melanogaster, the larval salivary glands produce secretory granules that are up to four-fold larger in

...
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1 Protocol feedback
Single-molecule Fluorescence in situ Hybridization (smFISH) for RNA Detection in Adherent Animal Cells
Authors:  Gal Haimovich and Jeffrey E. Gerst, date: 11/05/2018, view: 23759, Q&A: 1
Transcription and RNA decay play critical roles in the process of gene expression and the ability to accurately measure cellular mRNA levels is essential for understanding this regulation. Here, we describe a single-molecule fluorescent in situ hybridization (smFISH) method (as performed in Haimovich et al., 2017) that detects ...
More >
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