Gal Haimovich —BIO-PROTOCOL

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Jeffrey Gerst Molecular Genetics, Weizmann Institute of Science, Israel, Israel,
1 protocol

Alka Mehra Institute of Genomics and Integrative Biology
20 protocols

Amanda Garner 1 protocol

Anca Savulescu University of Cape Town
14 protocols

Annis Richardson University of California, Berkeley
5 protocols

Editor

Gal Haimovich

Post-Doc, Molecular Genetics, Weizmann Institute of Science, Israel

Research focus

Cell biology
mRNA localization, mRNA transfer, membrane nanotubes, exosomes

2 Author merit
Reviewer merit
Editor merit

Lab Protocol Hub

Gerst Lab

Education

PhD, Technion - Israeli institute of technology, 2011

Lab information

Jeffrey Gerst lab http://www.weizmann.ac.il/molgen/Gerst/
We are studying protein and mRNA localization in eukaryotic cells (mainly yeast & mammalian cells).
http://www.weizmann.ac.il/molgen/Gerst/

Publications

https://greenfluorescentblog.wordpress.com/about/list-of-publications/
Haimovich, G., Zabezhinsky, D., Haas, B., Slobodin, B., Purushothaman, P., Fan, L., Levin, J. Z., Nusbaum, C. and Gerst, J. E. (2016). Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to "MS2 coat proteins bound to yeast mRNAs block 5' to 3' degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system". RNA 22(5): 660-666.
Haimovich, G., Cohen-Zontag, O. and Gerst, J. E. (2016). A role for mRNA trafficking and localized translation in peroxisome biogenesis and function? Biochim Biophys Acta 1863(5): 911-921.
Buxbaum, A. R.*, Haimovich, G.* and Singer, R. H. (2015). In the right place at the right time: visualizing and understanding mRNA localization. Nat Rev Mol Cell Biol 16(2): 95-109. (*Equal contribution)
Somekh, J., Haimovich, G., Guterman, A., Dori, D. and Choder, M. (2014). Conceptual modeling of mRNA decay provokes new hypotheses. PLoS One 9(9): e107085.
Haimovich, G., Medina, D. A., Causse, S. Z., Garber, M., Millan-Zambrano, G., Barkai, O., Chavez, S., Perez-Ortin, J. E., Darzacq, X. and Choder, M. (2013). Gene expression is circular: factors for mRNA degradation also foster mRNA synthesis. Cell 153(5): 1000-1011.
Haimovich, G., Choder, M., Singer, R. H. and Trcek, T. (2013). The fate of the messenger is pre-determined: a new model for regulation of gene expression. Biochim Biophys Acta 1829(6-7): 643-653.
Harel-Sharvit, L., Eldad, N., Haimovich, G., Barkai, O., Duek, L. and Choder, M. (2010). RNA polymerase II subunits link transcription and mRNA decay to translation. Cell 143(4): 552-563.
Goler-Baron, V., Selitrennik, M., Barkai, O., Haimovich, G., Lotan, R. and Choder, M. (2008). Transcription in the nucleus and mRNA decay in the cytoplasm are coupled processes. Genes Dev 22(15): 2022-2027.
Lotan, R., Goler-Baron, V., Duek, L., Haimovich, G. and Choder, M. (2007). The Rpb7p subunit of yeast RNA polymerase II plays roles in the two major cytoplasmic mRNA decay mechanisms. J Cell Biol 178(7): 1133-1143.
Kamer, I., Sarig, R., Zaltsman, Y., Niv, H., Oberkovitz, G., Regev, L., Haimovich, G., Lerenthal, Y., Marcellus, R. C. and Gross, A. (2005). Proapoptotic BID is an ATM effector in the DNA-damage response. Cell 122(4): 593-603.
Weiss, M., Haimovich, G. and Pick, U. (2001). Phosphate and Sulfate uptake in the halotolerant alga Dunaliella are driven by Na+-symport mechanism. J Plant Physiology 158:1519-1525.

2 Protocols published

Single-molecule Fluorescence in situ Hybridization (smFISH) for RNA Detection in Adherent Animal Cells

Authors: Gal Haimovich and Jeffrey E. Gerst, date: 11/05/2018, view: 321, Q&A: 1

Transcription and RNA decay play critical roles in the process of gene expression and the ability to accurately measure cellular mRNA levels is essential for understanding this regulation. Here, we describe a single-molecule fluorescent in situ ...

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Plasmid Extract from Budding Yeast (Saccharomyces cerevisiae)

Author: Gal Haimovich, date: 07/20/2018, view: 587, Q&A: 0

Plasmids are widely used tools in yeast research. In many cases, plasmid libraries are used in genetic screens or in yeast two hybrid screens. In such cases, it is necessary to extract plasmids carrying unknown genetic elements from positive clones ...

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3 Protocols reviewed

Formation of Minimised Hairpin Template-transcribing Dumbbell Vectors for Small RNA Expression

Authors: Xiaoou Jiang and Volker Patzel, date: 06/05/2017, view: 3118, Q&A: 0

A major barrier for using non-viral vectors for gene therapy is the short duration of transgene expression in postmitotic tissues. Previous studies showed transgene expression from conventional plasmid fell to sub-therapeutic level shortly after ...

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A Golden Gate-based Protocol for Assembly of Multiplexed gRNA Expression Arrays for CRISPR/Cas9

Authors: Johan Vad-Nielsen, Lin Lin , Kristopher Torp Jensen, Anders Lade Nielsen and Yonglun Luo, date: 12/05/2016, view: 7204, Q&A: 1

The CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein 9 (Cas9) has become the most broadly used and powerful tool for genome editing. Many applications of CRISPR-Cas9 require the delivery of multiple small guide ...

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39 Protocols edited

A Method for SUMO Modification of Proteins in vitro

Authors: Christine C. Lee, Bing Li, Hongtao Yu and Michael J. Matunis, date: 10/05/2018, view: 435, Q&A: 0

The Small Ubiquitin-related Modifier (SUMO) is a protein that is post-translationally added to and reversibly removed from other proteins in eukaryotic cells. SUMO and enzymes of the SUMO pathway are well conserved from yeast to humans and SUMO ...

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Fluorophore-Based Mitochondrial Ca²⁺ Uptake Assay

Author: Charles B. Phillips, date: 07/20/2018, view: 475, Q&A: 0

The physiological importance of mitochondrial calcium uptake, observed in processes such as ATP production, intracellular calcium signaling, and apoptosis, makes desirable a simple, straightforward way of investigating this event with unambiguous ...

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