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Alka Mehra Institute of Genomics and Integrative Biology
18 protocols

Amanda Garner 1 protocol

Anca Savulescu University of Cape Town
11 protocols

Annis Richardson University of California, Berkeley
5 protocols

Editor
Gal Haimovich
  • Post-Doc, Molecular Genetics, Weizmann Institute of Science, Israel
Research focus
  • Cell biology
  • mRNA localization, mRNA transfer, membrane nanotubes, exosomes
  • 1 Author merit

Education

PhD, Technion - Israeli institute of technology, 2011

Lab information

Jeffrey Gerst lab http://www.weizmann.ac.il/molgen/Gerst/
We are studying protein and mRNA localization in eukaryotic cells (mainly yeast & mammalian cells).
http://www.weizmann.ac.il/molgen/Gerst/

Publications

1. Weiss M., Haimovich G. and Pick U. (2001) Phosphate and Sulfate uptake in the halotolerant alga Dunaliella are driven by Na+-symport mechanism. J. Plant Physiology, 158:1519-1525.
2. Kamer I., Sarig R., Zaltsman Y., Niv H., Oberkovitz G., Regev L., Haimovich G., Lerenthal Y., Marcellus RC. and Gross A. (2005) Pro-apoptotic BID is an ATM effector in the DNA damage response. Cell, 122(4):593-603.
3. Lotan R., Goler Bar-On V., Duek L., Haimovich G. and Choder M. (2007) The Rpb7p subunit of yeast RNA polymerase II plays roles in the two major cytoplasmic mRNA decay mechanisms. J. Cell Biology, 178(7):1133-1143.
4. Goler Bar-On V., Selitrennik M., Barkai O., Haimovich G., Lotan R. and Choder M. (2008) Transcription in the nucleus and mRNA decay in the cytoplasm are coupled processes. Genes and Development, 22:2022-2027.
5. Harel-Sharvit L., Eldad N., Haimovich G., Barkai O., Duek L. and Choder M. (2010) RNA polymerase II subunits link transcription and mRNA decay to translation. Cell, 143(4):552-563.
6. Haimovich G., Singer RH., Choder M. and Trček T. (2013) The fate of the messenger is pre-determined: a new model for regulation of gene expression. Biochem. Biophys. Acta - Gene Regulatory Mechanisms, 1829(6-7):643-653.
7. Haimovich G., Medina D., Causse S., Garber M., Millan-Zambrano G., Barkai O., Chavez S., Perez-Ortin JE., Darzacq X. and Choder M. (2013) Gene Expression Is Circular: Factors for mRNA Degradation Also Foster mRNA Synthesis. Cell, 153(5):1000-1011.
8. Somekh J., Haimovich G., Guterman A., Choder M. and Dori D. (2014) Conceptual Modeling of mRNA decay provokes new hypotheses. PLoS One, 9(9): e107085.
9. Buxbaum AR. *, Haimovich G. * and Singer RH. (2015) In the right place at the right time: visualizing and understanding mRNA localization. Nature Reviews Molecular Cell Biology, 16:95-109. * - first co-author (equal contribution).
10. Haimovich G., Cohen-Zontag O. and Gerst JE. (2016) A role for mRNA trafficking and localized translation in peroxisome biogenesis and function? Biochem. Biophys. Acta - Molecular Cell Research, 1863(5):911-921.
11. Haimovich G., Zabezhinsky D., Haas B., Slobodin B., Purushothaman P., Lin F., Levin JZ., Nusbaum C. and Gerst JE. (2016) Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to “MS2 coat protein bound to yeast mRNAs block 5’ to 3’ degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system”. RNA, 22:660-666.
12. Haimovich G., Ecker CM., Dunagin MC., Eggan E., Raj A., Gerst JE. and Singer RH. (2017) Intercellular mRNA trafficking via membrane nanotube-like extensions in mammalian cells. PNAS, 114(46): E9873–E9882.

3 Protocols reviewed
Ectopic Gene Expression in Macrophages Using in vitro Transcribed mRNA
Authors:  Pallavi Chandra and Jennifer A. Philips, date: 05/20/2018, view: 511, Q&A: 0
Macrophages are immune cells that contribute to host defense through various mechanisms including phagocytosis and antigen presentation. Their antimicrobial capacity is subverted by clinically important intracellular pathogens such as Mycobacterium ...
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Formation of Minimised Hairpin Template-transcribing Dumbbell Vectors for Small RNA Expression
Authors:  Xiaoou Jiang and Volker Patzel, date: 06/05/2017, view: 2452, Q&A: 0
A major barrier for using non-viral vectors for gene therapy is the short duration of transgene expression in postmitotic tissues. Previous studies showed transgene expression from conventional plasmid fell to sub-therapeutic level shortly after ...
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37 Protocols edited
An Improved Method for Measuring Chromatin-binding Dynamics Using Time-dependent Formaldehyde Crosslinking
Formaldehyde crosslinking is widely used in combination with chromatin immunoprecipitation (ChIP) to measure the locations along DNA and relative levels of transcription factor (TF)-DNA interactions in vivo. However, the measurements that ...
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Single-probe RNA FISH in Yeast
Authors:  Gable M. Wadsworth, Rasesh Y. Parikh and Harold D. Kim, date: 06/05/2018, view: 379, Q&A: 0
Quantitative profiling of mRNA expression is an important part of understanding the state of a cell. The technique of RNA Fluorescence In Situ Hybridization (FISH) involves targeting an RNA transcript with a set of 40 complementary ...
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