Jeffrey Gerst Weizmann Institute of Science Rehovot
2 protocols

Rohini Nair Weizmann Institute of Science Rehovot
1 protocol
Abhijit Kale LevitasBio
10 protocols

Akira Karasawa New York Structural Biology Center
10 protocols

Aksiniya Asenova University of California, Davis
17 protocols

Alexander Miethke Cincinnati Childrens Hospital Cincinnati
12 protocols

Editor
Gal Haimovich
  • Research scientist, Weizmann Institute of Science Rehovot
Research focus
  • mRNA localization, mRNA transfer, membrane nanotubes, exosomes
  • 3 Author merit

Education

PhD, Technion - Israeli institute of technology, 2011

Lab information

Jeffrey Gerst lab http://www.weizmann.ac.il/molgen/Gerst/
We are studying protein and mRNA localization in eukaryotic cells (mainly yeast & mammalian cells).
http://www.weizmann.ac.il/molgen/Gerst/

12 Protocols published
An Aptamer-based mRNA Affinity Purification Procedure (RaPID) for the Identification of Associated RNAs (RaPID-seq) and Proteins (RaPID-MS) in Yeast
Authors:  Rohini R. Nair, Gal Haimovich and Jeffrey E. Gerst, date: 01/05/2022, view: 1203, Q&A: 2

RNA-RNA and RNA-protein interactions are involved in the regulation of gene expression. Here, we describe an updated and extended version of our RNA purification and protein identification (RaPID) protocol for the pulldown of aptamer-tagged mRNAs by

...
Single-molecule Fluorescence in situ Hybridization (smFISH) for RNA Detection in Adherent Animal Cells
Authors:  Gal Haimovich and Jeffrey E. Gerst, date: 11/05/2018, view: 27495, Q&A: 2
Transcription and RNA decay play critical roles in the process of gene expression and the ability to accurately measure cellular mRNA levels is essential for understanding this regulation. Here, we describe a single-molecule fluorescent in situ ...
4 Protocols reviewed
Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
Authors:  Rachna Narayanan and Andrew C. Oates, date: 03/20/2019, view: 11277, Q&A: 0
In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes ...
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Ectopic Gene Expression in Macrophages Using in vitro Transcribed mRNA
Authors:  Pallavi Chandra and Jennifer A. Philips, date: 05/20/2018, view: 5930, Q&A: 0
Macrophages are immune cells that contribute to host defense through various mechanisms including phagocytosis and antigen presentation. Their antimicrobial capacity is subverted by clinically important intracellular pathogens such as Mycobacterium ...
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118 Protocols edited
Isolation of tdTomato Expressing Inter-follicular Epidermal Melanocytes or Keratinocytes from Mouse Tail Skin
Authors:  Samuel Pop, Oscar Urtatiz and Catherine D. Van Raamsdonk, date: 04/20/2022, view: 975, Q&A: 0

The epidermis is the outermost layer of the skin. It is made up of mostly keratinocytes along with a small number of melanocytes and Langerhans cells. Melanocytes produce a pigment called melanin, which is transferred to the keratinocytes, and

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Production of Recombinant Hepatitis B virus (HBV) and Detection of HBV in Infected Human Liver Organoids
Authors:  Tanvir Hossain, Shahla Romal and Tokameh Mahmoudi, date: 04/20/2022, view: 418, Q&A: 0

The absence of long term, primary untransformed in vitro models that support hepatitis B virus (HBV) infection and replication have hampered HBV pre-clinical research, which was reflected in the absence of a curative therapy until recently. One of

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1 Protocol feedback
Single-molecule Fluorescence in situ Hybridization (smFISH) for RNA Detection in Adherent Animal Cells
Authors:  Gal Haimovich and Jeffrey E. Gerst, date: 11/05/2018, view: 27495, Q&A: 2
Transcription and RNA decay play critical roles in the process of gene expression and the ability to accurately measure cellular mRNA levels is essential for understanding this regulation. Here, we describe a single-molecule fluorescent in situ hybridization (smFISH) method (as performed in Haimovich et al., 2017) that detects ...
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