Annarita Miccio UMR1163 Imagine Institute
1 protocol

Elisa Magrin
1 protocol

Anne Chalumeau
1 protocol

Giacomo Frati
  • Post-Doc, Chromatin and gene regulation upon differentiation Imagine Institute
Research focus
  • Stem cell
  • 1 Author merit

Education

PhD in Molecular medicine, University Vita-Salute San Raffaele, 2016

Publications

Chalumeau, A, Frati G, Miccio A. Reverse phase (RP)-high-performance liquid chromatography (HPLC) analysis of globin chains. Bio-protocol (2020). bio-protocol.org/prep420.



El Hoss S, Cochet S, Godard A, Yan H, Dussiot M, Frati G, Boutonnat-Faucher B, Laurance S, Renaud O, Joseph L, Miccio A, Brousse V, Mohandas N, El Nemer W. Fetal hemoglobin rescues ineffective erythropoiesis in sickle cell disease Haematologica (2020) https://doi.org/10.3324/haematol.2020.265462



Frati G Miccio A. A genome-editing system for therapeutical targeting of stem cells. Chapter in the volume entitled “Leukemic Stem Cells”, Methods in Molecular Biology. Doi: https://doi.org/10.1007/978-1-0716-0810-4



Weber L*, Frati G*; Felix T, Hardouin G, Casini A, Wollenschlaeger C, Meneghini V, Masson C, De Cian A, Chalumeau A, Mavilio F, Amendola M, Andre-Schmutz I, Cereseto A, El Nemer W, Concordet JP, Giovannangeli C, Cavazzana M, Miccio A. Editing a γ-Globin Repressor Binding Site Restores Fetal Hemoglobin Synthesis and Corrects the Sickle Cell Disease Phenotype Science Advances (2020), 6 (7), eaay9392. https://doi.org/10.1126/sciadv.aay9392.

* Contributed equally



Tykesson E, Hassinen A, Zielinska K, Thelin MA, Frati G, Ellervik U, Westergren-Thorsson G, Malmström A, Kellokumpu S, Maccarana M. Dermatan sulfate epimerase 1 and dermatan 4-O-sulfotransferase 1 form complexes that generate long epimerized 4-O-sulfated blocks. J. Biol. Chem. jbc.RA118.003875 (2018). doi:10.1074/jbc.RA118.003875



Frati G, Luciani M, Meneghini V, De Cicco S, Ståhlman M, Blomqvist M, Grossi S, Filocamo M, Morena F, Menegon A, Martino S, Gritti A. Human iPSC-based models highlight defective glial and neuronal differentiation from neural progenitor cells in metachromatic leukodystrophy. Cell Death Dis. 9, (2018)



Meneghini V*, Frati G*, Sala D, De Cicco S, Luciani M, Cavazzin C, Paulis M, Mentzen W, Morena F, Giannelli S, Sanvito F, Villa A, Bulfone A, Broccoli V, Martino S, Gritti A. Generation of Human Induced Pluripotent Stem Cell-Derived Bona Fide Neural Stem Cells for Ex Vivo Gene Therapy of Metachromatic Leukodystrophy. STEM CELLS Transl. Med. 6, 352–368 (2017).

*Contributed equally
1 Protocol published
Reverse Phase-high-performance Liquid Chromatography (RP-HPLC) Analysis of Globin Chains from Human Erythroid Cells
Authors:  Anne Chalumeau, Giacomo Frati, Elisa Magrin and Annarita Miccio, date: 01/20/2021, view: 748, Q&A: 0

β-hemoglobinopathies are severe genetic disorders characterized either by the abnormal synthesis of the adult β-globin chains of the hemoglobin (Hb) tetramer (βS-globin chains) in sickle cell disease (SCD) or by the reduced

...
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.