André Rey-Cibati Instituto de Estudios Avanzados (IDEA), 2020-
1 protocol

Gustavo Benaim
  • Professor, Instituto de Estudios Avanzados (IDEA)., Instituto de Estudios Avanzados (IDEA), Venezuela, 2002-2020; Head of Laboratoy of Biophysics, Instituto de Biologia Experimental. Faculty of Science, Universidad Central de Venezuela, Venezuela, 1992-2020; Head of Laboratory of Cell Signaling and Biochemistry of Parasites. , Instituto de Estudios Avanzados (IDEA). , Instituto de Estudios Avanzados (IDEA). , Venezuela, 2000-2020; Professor, Instituto de Estudios Avanzados (IDEA). , Instituto de Estudios Avanzados (IDEA). , Venezuela, 2000-2000
Research focus
  • Cell biology
  • 1 Author merit

Education

PhD, "Doctor in Science" - Area: Cell Biology. Faculty of Science. Universidad Central de Venezuela and Instituto de Ciencias Biomedicas. Universidade Federal de Rio de aneiro. Brazil, 1988

Publications

Since 2015
• Stateva, S.R., Salas, V., Benaim, G., Menéndez, M., Solís, D. & Villalobo, A. (2015) Characterization of phospho-(tyrosine)-mimetic calmodulin mutants. PLoS One Apr 1;10(4):e0120798. doi: 10.1371/journal.pone.0120798. eCollection 2015.
• Paniz-Mondolfi, A., Rajesh S., Jour, G., Mahmoodi, M., Diwan, A.H., Barkoh, B.A., Cason, R., Huttenbach, Y., Benaim, G., Galbiacea, J. & Luthra, R. (2015) Cutaneous carcinosarcoma and the EMT: to transition, or not to transition? That is the question. (2015) Virchows Archiv. Eur. J. Pathol. 466: 359-60.
• Veiga-Santos, P., Li, K., Lameira, L., de Carvalho, T.M., Huang, G., Galizzi, M., Shang, N., Li, Q., Gonzalez-Pacanowska, D., Hernandez-Rodriguez, V., Benaim, G., Guo, R.T., Urbina, J.A., Docampo, R., de Souza, W. & Oldfield, E. (2015) SQ109: A New Drug Lead for Chagas Disease. Antimicrob. Agents Chemother. 59: 50-61
• Stateva, S.R., Salas, V., Anguita, E., Benaim, G. & Villalobo, A. (2015) Ca2+/Camodulin and Apo-Calmodulin Both Bind to and Enhance the Tyrosine Kinase Activity of c-Src. PLoS One June, 9 ;10 (6):e0128783. doi: 10.1371/journal.pone.0128783. eCollection 2015. PMID: 26058065
• Stateva, S.R., Salas, V., Benguría, A., Cossío, I., Anguita, E., Martín-Nieto, J., Benaim, G. & Villalobo, A. (2015).The Activating Role of Phospho-(Tyr)-Calmodulin on the Epidermal Growth Factor Receptor. Biochem. J. 472: 195-204
• Figarella, K., Marsiccobetre, S., Arocha, I., Colina, W., Hasegawa, M., Rodriguez, M., Duszenko, M., Benaim, G. & Uzcategui, N. L. (2016) Synthesis of Ergosterone-coupled Triazol and characterization of their biological activity on Leishmania mexicana promastigotes: Induction of mitochondrial dysfunction, oxidative stress, and acidocalcisomes Ca2+ release. Microbial Cell. 3: 14-28
• Sbaraglini, M.L., Vanrell, M.C., Bellera, C.L., Benaim, G., Carrillo, C., Talevi, A. & Romano, P.S. (2016). Drug repositioning for neglected tropical protozoan diseases. Curr. Top. Med. Chem. 16: 2201-2222
• Benaim, G., Pimentel, A.A., Felibertt, P., Mayora, A., Colman, L., Sojo, F., Rojas, H., & De Sanctis, J.B. (2016). Sphingosine inhibits the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) activity. Biochem. Biophys. Res. Commun. 473: 572-577
• García-García, V., Oldfield, E. & Benaim, G, (2016) Inhibition of Leishmania mexicana Growth by the Tuberculosis Drug SQ109. Antimicrob. Agents Chemother. 60: 6386-6389
• Pérez-Gordones, M.C., Ramírez-Iglesias, J.R., Cervino, V., Uzcanga, G.L., Benaim, G., & Mendoza, M. (2017) Evidence of the presence of a calmodulin-sensitive plasma membrane Ca2+-ATPase in Trypanosoma equiperdum. Mol. Biochem. Parasitol. 213:1-11.
• De Gouveia Y., Hernández-Rodríguez V., Rodríguez-Durán J., Suárez A., & Benaim, G., (2017) Efecto de un metilen-dioxiflavonoide aislado de Iresine diffusa sobre la viabilidad celular de Leishmania mexicana y la homeostasis intracelular del Ca2+. Invest. Clín. 58: 334-351.
• Pinto-Martinez, A., Rodriguez-Durán, J., Serrano-Martin, X., Hernandez-Rodriguez, V. & Benaim, G. (2018) Mechanism of Action of Miltefosine on Leishmania donovani Involves the Impairment of Acidocalcisome Function and the Activation of the Sphingosine-Dependent Plasma Membrane Ca2+ Channel. Antimicrob Agents Chemother. 62: 1-10
• Valdivieso, E., Torrealba, C., Mejias, F., Sojo, F., Rojas-Ruiz, F., Arvelo, F., Benaim, G., Kouznetsov, V & Dagger, V. (2018) In vitro 4-Aryloxy-7-chloroquinoline derivatives are effective in mono- and combined therapy against Leishmania donovani and induce mitocondrial membrane potential disruption. Acta Tropíca 186: 36-42.
• Pinto-Martinez, A., Hernandez-Rodriguez, V.,Rodriguez-Duran, J. Hejchman, E. & Benaim, G. (2018) Anti-Trypanosoma cruzi action of a new benzofuran derivative based structure. Exp. Parasitol. 189: 8-15
• Ramírez-Iglesias, J.R., Pérez-Gordones, M.C. del Castillo, J.R. Mijares, A. Benaim, G. & Mendoza, M. (2018) Identification and characterization of a calmodulin binding domain in the plasma membrane Ca2+-ATPase from Trypanosoma equiperdum. Mol. Biochem. Parasitol. 222: 51-60
• Rodríguez, S., Silva, M., Benaím, G. & Bubis, J. (2018) Phosphorylation-Induced Conformational Changes of Photoactivated Rhodopsin Probed by Fluorescent Labeling at Cys140 and Cys316 Biochemie 150: 57-69
• Moghaddam, P.A., Singh, R., Mahmoodi, M., Mehrotra, M., Benaim, G., Luthra, R. & Paniz-Mondolfi, A. (2018) Poorly differentiated osteoclast-like giant cell variant of cutaneous squamous cell carcinoma: uncovering its mutational landscape through massive parallel sequencing. Pathol. Res. Pract. 214 : 1898-1903
• Grillet, M.E., Hernández, J.V., Llewellyn, M., Paniz-Mondolfi, A., Tami, A.,Vincenti, F., Marquez, M., Mogollon-Mendoza, A. Hernandez-Pereira, C., Plaza-Morr, J., Blohm, G., Grijalva, M., Costales, J.A., Fergusson, H., Schwabl, P., Castro, L.H., Lamberton, P., Streiker, D., Haydon, D., Miles, M.A., Segovia, M., Acosta-Serrano, A., Acquattelam H., Basáñez, M.G., Benaim, G., Conn, J.E., Freilij, H., Hotez, P., Kato, H., Lednicky, J., Mas-Coma, S., Morris, J.E., Navarro, J.C., Urbina, J.A., Villegas, L., Martinez, C.E., Rodríguez, M., Carrasco, H., Crainey, L., Luz, S., Moreno, J., Noya. O., Ramírez, J.D., Alarcón-de Noya, B. (2019) Venezuela’s humanitarian crisis, resurgence of vector-borne diseases and implications for spillover in the region: a call for action. Lancet Infect. Dis. 3099: 30757-30756.
• Martinez-Sotillo, N., Pinto-Martinez, A., Hejchman, E. & Benaim, G. (2019) Antiproliferative effect of a benzofuran derivate based on the structure of amiodarone on Leishmania donovani affecting mitochondria, acidocalcisomes and intracellular Ca2+ homeostasis. Parasitol. Int. 70: 112-117
• Rodriguez-Duran, J., Pinto-Martinez, A., Castillo, C. & Benaim, G. (2019) Identification and Electrophysiological Properties of a Sphingosine-dependent Plasma Membrane Ca2+ Channel in Trypanosoma cruzi. FEBS J. 286: 3909-3925. doi: 10.1111/febs.14947
• Madigan, R., Majoy, S., Ritter, K., Concepción, J. L., Márquez, M. E., Silva, S. C., Zao, C. L., Pérez-Alvarez, A., Rodriguez-Morales, A., Mogollón-Mendoza, A. C.,Scot Estep, J., Benaím, G., & Paniz-Mondolfi, A.E. (2019) Successful treatment of canine Chagas’ Disease using combination treatment of Amiodarone and Itraconazole. J. Am. Vet. Med. Assoc. 255: 317-329
• Gil, Z., Martinez-Sotillo, N., Pinto-Martinez, A., Mejias, F., Martinez, J.C., Galindo-Castro, I., Oldfield, E. & Benaim, G. (2020) SQ109 inhibits proliferation of Leishmania donovani disruption of the intracellular Ca2+ homeostasis collapsing the mitochondrial electrochemical potential (ΔΨm) and affecting acidocalcisomes. Parasitol. Res. 119: 649-65782
• Benaim, G., Paniz-Mondolfi, Sordillo, E.M. & Martinez-Sotillo, N. (2020) Disruption of intracellular calcium homeostasis as a therapeutic target against Trypanosoma cruzi. Review. Frontiers Cell. Infect. Microbiol.10: 46. doi: 10.3389/fcimb.2020.00046
• Benaim, G., Alberto Paniz-Mondolfi y Emilia Mia Sordillo (2020) Rationale for Use of Amiodarone and its Derivatives for Treatment of Chagas´ Disease and Leishmaniasis. Review. Curr. Pharmac. Design (in press)
1 Protocol published
Determination of Intracellular Ca2+ Concentration in the Human Pathogens Trypanosomatids Leishmania mexicana and Trypanosoma cruzi by the Use of the Fluorescent Ca2+ Indicator Fura-2
Authors:  Andre Rey-Cibati, Mariana Valladares-Delgado and Gustavo Benaim, date: 09/20/2020, view: 275, Q&A: 0
Ca2+ is an essential signaling messenger in all eukariotic cells, playing a pivotal role in many cellular functions as cell growth control (differentiation, fertilization and apoptosis), secretion, gene expression, enzyme regulation, ...
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