Tsukasa Okiyoneda
  • Faculty, Kwansei Gakuin University
Research focus
  • Biochemistry
  • Cystic Fibrosis, CFTR, protein quality control
  • 1 Author merit


Ph.D., Kumamoto University, 2003

Lab information

Tsukasa Okiyoneda Lab


• Sakai, R., Fukuda, R., Unida, S., Aki, M., Ono, Y., Endo, A., Kusumi, S., Koga, D., Fukushima, T., Komada, M. and Okiyoneda, T. (2019). The integral function of the endocytic recycling compartment is regulated by RFFL-mediated ubiquitylation of Rab11 effectors. J Cell Sci 132(3).
• Fukuda, R. and Okiyoneda, T. (2018). Peripheral Protein Quality Control as a Novel Drug Target for CFTR Stabilizer. Front Pharmacol 9: 1100.
• Okiyoneda, T., Veit, G., Sakai, R., Aki, M., Fujihara, T., Higashi, M., Susuki-Miyata, S., Miyata, M., Fukuda, N., Yoshida, A., Xu, H., Apaja, P. M. and Lukacs, G. L. (2018). Chaperone-Independent Peripheral Quality Control of CFTR by RFFL E3 Ligase. Dev Cell 44(6): 694-708 e697.
• Bagdany, M., Veit, G., Fukuda, R., Avramescu, R. G., Okiyoneda, T., Baaklini, I., Singh, J., Sovak, G., Xu, H., Apaja, P. M., Sattin, S., Beitel, L. K., Roldan, A., Colombo, G., Balch, W., Young, J. C. and Lukacs, G. L. (2017). Chaperones rescue the energetic landscape of mutant CFTR at single molecule and in cell. Nat Commun 8(1): 398.
• Okiyoneda, T. (2014). [Peripheral quality control mechanism eliminating aberrant proteins from the plasma membrane]. Seikagaku 86(1): 54-58.
• Apaja, P. M., Foo, B., Okiyoneda, T., Valinsky, W. C., Barriere, H., Atanasiu, R., Ficker, E., Lukacs, G. L. and Shrier, A. (2013). Ubiquitination-dependent quality control of hERG K+ channel with acquired and inherited conformational defect at the plasma membrane. Mol Biol Cell 24(24): 3787-3804.
• Okiyoneda, T., Veit, G., Dekkers, J. F., Bagdany, M., Soya, N., Xu, H., Roldan, A., Verkman, A. S., Kurth, M., Simon, A., Hegedus, T., Beekman, J. M. and Lukacs, G. L. (2013). Mechanism-based corrector combination restores DeltaF508-CFTR folding and function. Nat Chem Biol 9(7): 444-454.
• Okiyoneda, T. and Lukacs, G. L. (2012). Fixing cystic fibrosis by correcting CFTR domain assembly. J Cell Biol 199(2): 199-204.
• Rabeh, W. M., Bossard, F., Xu, H., Okiyoneda, T., Bagdany, M., Mulvihill, C. M., Du, K., di Bernardo, S., Liu, Y., Konermann, L., Roldan, A. and Lukacs, G. L. (2012). Correction of both NBD1 energetics and domain interface is required to restore DeltaF508 CFTR folding and function. Cell 148(1-2): 150-163.
• Sato, T., Sako, Y., Sho, M., Momohara, M., Suico, M. A., Shuto, T., Nishitoh, H., Okiyoneda, T., Kokame, K., Kaneko, M., Taura, M., Miyata, M., Chosa, K., Koga, T., Morino-Koga, S., Wada, I. and Kai, H. (2012). STT3B-dependent posttranslational N-glycosylation as a surveillance system for secretory protein. Mol Cell 47(1): 99-110.
• Barriere, H., Apaja, P., Okiyoneda, T. and Lukacs, G. L. (2011). Endocytic sorting of CFTR variants monitored by single-cell fluorescence ratiometric image analysis (FRIA) in living cells. Methods Mol Biol 741: 301-317.
• Okiyoneda, T., Apaja, P. M. and Lukacs, G. L. (2011). Protein quality control at the plasma membrane. Curr Opin Cell Biol 23(4): 483-491.
• Peters, K. W., Okiyoneda, T., Balch, W. E., Braakman, I., Brodsky, J. L., Guggino, W. B., Penland, C. M., Pollard, H. B., Sorscher, E. J., Skach, W. R., Thomas, P. J., Lukacs, G. L. and Frizzell, R. A. (2011). CFTR Folding Consortium: methods available for studies of CFTR folding and correction. Methods Mol Biol 742: 335-353.
• Hutt, D. M., Herman, D., Rodrigues, A. P., Noel, S., Pilewski, J. M., Matteson, J., Hoch, B., Kellner, W., Kelly, J. W., Schmidt, A., Thomas, P. J., Matsumura, Y., Skach, W. R., Gentzsch, M., Riordan, J. R., Sorscher, E. J., Okiyoneda, T., Yates, J. R., 3rd, Lukacs, G. L., Frizzell, R. A., Manning, G., Gottesfeld, J. M. and Balch, W. E. (2010). Reduced histone deacetylase 7 activity restores function to misfolded CFTR in cystic fibrosis. Nat Chem Biol 6(1): 25-33.
• Barriere, H., Bagdany, M., Bossard, F., Okiyoneda, T., Wojewodka, G., Gruenert, D., Radzioch, D. and Lukacs, G. L. (2009). Revisiting the role of cystic fibrosis transmembrane conductance regulator and counterion permeability in the pH regulation of endocytic organelles. Mol Biol Cell 20(13): 3125-3141.
• Glozman, R., Okiyoneda, T., Mulvihill, C. M., Rini, J. M., Barriere, H. and Lukacs, G. L. (2009). N-glycans are direct determinants of CFTR folding and stability in secretory and endocytic membrane traffic. J Cell Biol 184(6): 847-862.
• Hashimoto, Y., Okiyoneda, T., Harada, K., Ueno, K., Sugahara, T., Yamashita, A., Shuto, T., Suico, M. A. and Kai, H. (2008). Phosphatidic acid metabolism regulates the intracellular trafficking and retrotranslocation of CFTR. Biochim Biophys Acta 1783(1): 153-162.
• Okiyoneda, T., Niibori, A., Harada, K., Kohno, T., Michalak, M., Duszyk, M., Wada, I., Ikawa, M., Shuto, T., Suico, M. A. and Kai, H. (2008). Role of calnexin in the ER quality control and productive folding of CFTR; differential effect of calnexin knockout on wild-type and DeltaF508 CFTR. Biochim Biophys Acta 1783(9): 1585-1594.
• Harada, K., Okiyoneda, T., Hashimoto, Y., Oyokawa, K., Nakamura, K., Suico, M. A., Shuto, T. and Kai, H. (2007). Curcumin enhances cystic fibrosis transmembrane regulator expression by down-regulating calreticulin. Biochem Biophys Res Commun 353(2): 351-356.
• Okiyoneda, T. and Lukacs, G. L. (2007). Cell surface dynamics of CFTR: the ins and outs. Biochim Biophys Acta 1773(4): 476-479.
• Harada, K., Okiyoneda, T., Hashimoto, Y., Ueno, K., Nakamura, K., Yamahira, K., Sugahara, T., Shuto, T., Wada, I., Suico, M. A. and Kai, H. (2006). Calreticulin negatively regulates the cell surface expression of cystic fibrosis transmembrane conductance regulator. J Biol Chem 281(18): 12841-12848.
• Okiyoneda, T., Kono, T., Niibori, A., Harada, K., Kusuhara, H., Takada, T., Shuto, T., Suico, M. A., Sugiyama, Y. and Kai, H. (2006). Calreticulin facilitates the cell surface expression of ABCG5/G8. Biochem Biophys Res Commun 347(1): 67-75.
• Shuto, T., Furuta, T., Oba, M., Xu, H., Li, J. D., Cheung, J., Gruenert, D. C., Uehara, A., Suico, M. A., Okiyoneda, T. and Kai, H. (2006). Promoter hypomethylation of Toll-like receptor-2 gene is associated with increased proinflammatory response toward bacterial peptidoglycan in cystic fibrosis bronchial epithelial cells. FASEB J 20(6): 782-784.
• Okiyoneda, T., Niibori, A., Harada, K., Kohno, T., Hashimoto, Y., Kusuhara, H., Takada, T., Shuto, T., Suico, M. A., Sugiyama, Y. and Kai, H. (2006). Bafilomycin A1-sensitive pathway is required for the maturation of cystic fibrosis transmembrane conductance regulator. Biochim Biophys Acta 1763(10): 1017-1023.
• Shuto, T., Kato, K., Mori, Y., Viriyakosol, S., Oba, M., Furuta, T., Okiyoneda, T., Arima, H., Suico, M. A. and Kai, H. (2005). Membrane-anchored CD14 is required for LPS-induced TLR4 endocytosis in TLR4/MD-2/CD14 overexpressing CHO cells. Biochem Biophys Res Commun 338(3): 1402-1409.
• Koga, T., Suico, M. A., Nakamura, H., Taura, M., Lu, Z., Shuto, T., Okiyoneda, T. and Kai, H. (2005). Sp1-dependent regulation of Myeloid Elf-1 like factor in human epithelial cells. FEBS Lett 579(13): 2811-2816.
• Shimohara, S., Murakami, T., Morikawa, M., Matsuo, J., Nagayama, S., Shuto, T., Suico, M. A., Okiyoneda, T., Yamatsu, I., Mizushima, T., Shimasaki, T. and Kai, H. (2005). Vitamins K1 and K2 potentiate hyperthermia by down-regulating Hsp72 expression in vitro and in vivo. Int J Oncol 27(6): 1527-1533.
• Kondo, H., Uchiyama, K. and Okiyoneda, T. (2004). [p97-mediated membrane fusion is required for Golgi and ER assembly]. Tanpakushitsu Kakusan Koso 49(7 Suppl): 916-919.
• Nagayama, S., Kai, H., Okiyoneda, T., Horikawa, S. and Miyata, T. (1999). Characterization of CFTR expression in a human pulmonary mucoepidermoid carcinoma cell line, NCI-H292 cells. FEBS Lett 455(3): 215-218.
• Okiyoneda, T., Harada, K., Takeya, M., Yamahira, K., Wada, I., Shuto, T., Suico, M. A., Hashimoto, Y. and Kai, H. (2004). Delta F508 CFTR pool in the endoplasmic reticulum is increased by calnexin overexpression. Mol Biol Cell 15(2): 563-574.
• Okiyoneda, T., Harada, K., Yamahira, K., Wada, I., Hashimoto, Y., Ueno, K., Suico, M. A., Shuto, T. and Kai, H. (2004). Characterization of the trafficking pathway of cystic fibrosis transmembrane conductance regulator in baby hamster kidney cells. J Pharmacol Sci 95(4): 471-475.
• Suico, M. A., Koga, T., Shuto, T., Hisatsune, A., Lu, Z., Basbaum, C., Okiyoneda, T. and Kai, H. (2004). Sp1 is involved in the transcriptional activation of lysozyme in epithelial cells. Biochem Biophys Res Commun 324(4): 1302-1308.
• Okiyoneda, T. and Kai, H. (2002). [Introduction of the conventional method of DNA transfection by adenovirus vector]. Nihon Yakurigaku Zasshi 119(6): 337-344.
• Okiyoneda, T., Wada, I., Jono, H., Shuto, T., Yoshitake, K., Nakano, N., Nagayama, S., Harada, K., Isohama, Y., Miyata, T. and Kai, H. (2002). Calnexin Delta 185-520 partially reverses the misprocessing of the Delta F508 cystic fibrosis transmembrane conductance regulator. FEBS Lett 526(1-3): 87-92.
1 Protocol published
ELISA Based Protein Ubiquitylation Measurement
Authors:  Yuka Kamada, Ryosuke Fukuda and Tsukasa Okiyoneda, date: 11/20/2019, view: 368, Q&A: 0
Ubiquitylation is a common post-translational modification of cellular proteins that results in proteasomal and lysosomal degradations. Ubiquitylation is generally measured by methods such as immunoblotting using anti-ubiquitin antibodies after ...
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