Maki Kawai-Yamada Graduate School of Science and Engineering, Saitama University, JAPAN
2 protocols

Yuma Ishikawa Graduate School of Science and Engineering, Saitama University, Saitama
1 protocol
Gazala Ameen North Dakota State University
5 protocols

María Martin Instituto de Investigaciones en Biodiversidad y Biotecnología. INBIOTEC-CONICET y FIBA
3 protocols

Reviewer
Shin‐nosuke Hashida
  • Research scientist, Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry
Research focus
  • -
  • 2 Author merit
2 Protocols published
Measurement of Chloroplastic NAD Kinase Activity and Whole Tissue NAD Kinase Assay
Authors:  Yuuma Ishikawa, Maki Kawai-Yamada and Shin-nosuke Hashida, date: 01/05/2020, view: 1603, Q&A: 0
Nicotinamide adenine dinucleotide phosphate (NADP) synthesis requires nicotinamide adenine dinucleotide (NAD) kinase activity, substrate NAD and ATP. The NAD kinase responds to various environmental stimuli and its activity is regulated via various ...
Detection of Disulfides in Protein Extracts of Arabidopsis thaliana Using Monobromobimane (mBB)
Authors:  Shin‐nosuke Hashida and Maki Kawai-Yamada, date: 03/05/2019, view: 3033, Q&A: 0
Thiol-disulfide exchange is a key posttranslational modification, determining the folding process of intra- and inter-protein structures. Thiols can be detected by colorimetric reagents, which are stoichiometrically reduced by free thiols, and by ...
1 Protocol reviewed
Ratiometric Measurement of Protein Abundance after Transient Expression of a Transgene in Nicotiana benthamiana
Authors:  Aashima Khosla and David C. Nelson, date: 09/05/2020, view: 582, Q&A: 0
Ratiometric reporters are tools to dynamically measure the relative abundance of a protein of interest. In these systems, a target protein fused to a fluorescent or bioluminescent reporter is expressed with fixed stoichiometry to a reference protein ...
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