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Maki Kawai-Yamada
Graduate School of Science and Engineering, Saitama University, JAPAN
2 protocols
Yuma Ishikawa
Graduate School of Science and Engineering, Saitama University, Saitama
1 protocol
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Gazala Ameen
South Dakota State University
5 protocols
María Martin
Instituto de Investigaciones en Biodiversidad y Biotecnología- National Scientific and Technical Research Council. INBIOTEC-CONICET
4 protocols
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Reviewer
Shin‐nosuke Hashida
Research scientist, Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry
Research focus
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2
Author merit
Reviewer merit
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Education
Ph.D., Department of Agriculture, Hokkaido University, Japan, 2005
Publications
https://www.scopus.com/authid/detail.uri?authorId=7006275663
http://orcid.org/0000-0003-1510-0410
http://www.researcherid.com/rid/A-6971-2013
2 Protocols published
Measurement of Chloroplastic NAD Kinase Activity and Whole Tissue NAD Kinase Assay
Authors:
Yuuma Ishikawa
,
Maki Kawai-Yamada
and
Shin-nosuke Hashida
,
date:
01/05/2020,
view:
3248,
Q&A:
0
Nicotinamide adenine dinucleotide phosphate (NADP) synthesis requires nicotinamide adenine dinucleotide (NAD) kinase activity, substrate NAD and ATP. The NAD kinase responds to various environmental stimuli and its activity is regulated via various ...
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Detection of Disulfides in Protein Extracts of
Arabidopsis thaliana
Using Monobromobimane (mBB)
Authors:
Shin‐nosuke Hashida
and
Maki Kawai-Yamada
,
date:
03/05/2019,
view:
4797,
Q&A:
0
Thiol-disulfide exchange is a key posttranslational modification, determining the folding process of intra- and inter-protein structures. Thiols can be detected by colorimetric reagents, which are stoichiometrically reduced by free thiols, and by ...
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2 Protocols reviewed
A Protocol for Flavonols, Kaempferol and Quercetin, Staining in Plant Root Tips
Author:
Nguyen Hoai Nguyen
,
date:
10/05/2020,
view:
2878,
Q&A:
0
Flavonols are a subclass of flavonoids of the group of plant secondary metabolites.
In planta
, flavonols play various functions such as antioxidant and natural regulator of auxin polar transport. Many lines of evidence have shown that ...
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Ratiometric Measurement of Protein Abundance after Transient Expression of a Transgene in
Nicotiana benthamiana
Authors:
Aashima Khosla
and
David C. Nelson
,
date:
09/05/2020,
view:
3608,
Q&A:
0
Ratiometric reporters are tools to dynamically measure the relative abundance of a protein of interest. In these systems, a target protein fused to a fluorescent or bioluminescent reporter is expressed with fixed stoichiometry to a reference protein ...
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