Shin‐nosuke Hashida —BIO-PROTOCOL

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Maki Kawai-Yamada Graduate School of Science and Engineering, Saitama University, JAPAN
2 protocols

Yuma Ishikawa Graduate School of Science and Engineering, Saitama University, Saitama
1 protocol

Gazala Ameen Washington State University
5 protocols

María Martin Instituto de Investigaciones en Biodiversidad y Biotecnología- National Scientific and Technical Research Council. INBIOTEC-CONICET
4 protocols

Reviewer

Shin‐nosuke Hashida

Research scientist, Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry

Research focus

2 Author merit
Reviewer merit
Editor merit

Education

Ph.D., Department of Agriculture, Hokkaido University, Japan, 2005

Publications

https://www.scopus.com/authid/detail.uri?authorId=7006275663

http://orcid.org/0000-0003-1510-0410
http://www.researcherid.com/rid/A-6971-2013

2 Protocols published

Measurement of Chloroplastic NAD Kinase Activity and Whole Tissue NAD Kinase Assay

Authors: Yuuma Ishikawa, Maki Kawai-Yamada and Shin-nosuke Hashida, date: 01/05/2020, view: 2419, Q&A: 0

Nicotinamide adenine dinucleotide phosphate (NADP) synthesis requires nicotinamide adenine dinucleotide (NAD) kinase activity, substrate NAD and ATP. The NAD kinase responds to various environmental stimuli and its activity is regulated via various ...

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Detection of Disulfides in Protein Extracts of Arabidopsis thaliana Using Monobromobimane (mBB)

Authors: Shin‐nosuke Hashida and Maki Kawai-Yamada, date: 03/05/2019, view: 3853, Q&A: 0

Thiol-disulfide exchange is a key posttranslational modification, determining the folding process of intra- and inter-protein structures. Thiols can be detected by colorimetric reagents, which are stoichiometrically reduced by free thiols, and by ...

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2 Protocols reviewed

A Protocol for Flavonols, Kaempferol and Quercetin, Staining in Plant Root Tips

Author: Nguyen Hoai Nguyen, date: 10/05/2020, view: 1409, Q&A: 0

Flavonols are a subclass of flavonoids of the group of plant secondary metabolites. In planta, flavonols play various functions such as antioxidant and natural regulator of auxin polar transport. Many lines of evidence have shown that ...

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Ratiometric Measurement of Protein Abundance after Transient Expression of a Transgene in Nicotiana benthamiana

Authors: Aashima Khosla and David C. Nelson, date: 09/05/2020, view: 2385, Q&A: 0

Ratiometric reporters are tools to dynamically measure the relative abundance of a protein of interest. In these systems, a target protein fused to a fluorescent or bioluminescent reporter is expressed with fixed stoichiometry to a reference protein ...

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