Bogumil Karas 1 protocol

Samuel Slattery Department of Biochemistry, Schulich School of Medicine & Dentistry, Western University, Canada, Canada,
1 protocol

Helen Wang Department of Biochemistry, Schulich School of Medicine & Dentistry, Western University, Canada, Canada,
1 protocol

David R Edgell
  • Professor and Acting Chair, Department of Biochemistry, Department of Biochemistry, Schulich School of Medicine & Dentistry, The University of Western Ontario, Canada, Canada,
Research focus
  • Genome editing, synthetic biology, mobile genetic elements
  • 1 Author merit


PhD, Dalhousie


Pereira CV, Bacman SR, Arguello T, Zekonyte U, Williams SL, Edgell DR, Moraes
CT. mitoTev-TALE: a monomeric DNA editing enzyme to reduce mutant mitochondrial
DNA levels. EMBO Mol Med. 2018 Jul 16. pii: e8084. doi: 10.15252/emmm.201708084.
[Epub ahead of print] PubMed PMID: 30012581.

Kaiser JC, King AN, Grigg JC, Sheldon JR, Edgell DR, Murphy MEP, Brinsmade SR,
Heinrichs DE. Repression of branched-chain amino acid synthesis in Staphylococcus
aureus is mediated by isoleucine via CodY, and by a leucine-rich attenuator
peptide. PLoS Genet. 2018 Jan 22;14(1):e1007159. doi:
10.1371/journal.pgen.1007159. eCollection 2018 Jan. PubMed PMID: 29357354; PubMed
Central PMCID: PMC5794164.

Slattery SS, Diamond A, Wang H, Therrien JA, Lant JT, Jazey T, Lee K, Klassen
Z, Desgagné-Penix I, Karas BJ, Edgell DR. An Expanded Plasmid-Based Genetic
Toolbox Enables Cas9 Genome Editing and Stable Maintenance of Synthetic Pathways
in Phaeodactylum tricornutum. ACS Synth Biol. 2018 Feb 16;7(2):328-338. doi:
10.1021/acssynbio.7b00191. Epub 2018 Jan 8. PubMed PMID: 29298053.

Guha TK, Edgell DR. Applications of Alternative Nucleases in the Age of
CRISPR/Cas9. Int J Mol Sci. 2017 Nov 29;18(12). pii: E2565. doi:
10.3390/ijms18122565. Review. PubMed PMID: 29186020; PubMed Central PMCID:

Wolfs JM, Hamilton TA, Lant JT, Laforet M, Zhang J, Salemi LM, Gloor GB,
Schild-Poulter C, Edgell DR. Biasing genome-editing events toward precise length
deletions with an RNA-guided TevCas9 dual nuclease. Proc Natl Acad Sci U S A.
2016 Dec 27;113(52):14988-14993. doi: 10.1073/pnas.1616343114. Epub 2016 Dec 12.
PubMed PMID: 27956611; PubMed Central PMCID: PMC5206545.

Roy AC, Wilson GG, Edgell DR. Perpetuating the homing endonuclease life cycle:
identification of mutations that modulate and change I-TevI cleavage preference.
Nucleic Acids Res. 2016 Sep 6;44(15):7350-9. doi: 10.1093/nar/gkw614. Epub 2016
Jul 7. PubMed PMID: 27387281; PubMed Central PMCID: PMC5009752.

Wolfs JM, DaSilva M, Meister SE, Wang X, Schild-Poulter C, Edgell DR.
MegaTevs: single-chain dual nucleases for efficient gene disruption. Nucleic
Acids Res. 2014 Jul;42(13):8816-29. doi: 10.1093/nar/gku573. Epub 2014 Jul 10.
PubMed PMID: 25013171; PubMed Central PMCID: PMC4117789.

McMurrough TA, Dickson RJ, Thibert SM, Gloor GB, Edgell DR. Control of
catalytic efficiency by a coevolving network of catalytic and noncatalytic
residues. Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):E2376-83. doi:
10.1073/pnas.1322352111. Epub 2014 May 27. PubMed PMID: 24912189; PubMed Central
PMCID: PMC4060692.

Kleinstiver BP, Wang L, Wolfs JM, Kolaczyk T, McDowell B, Wang X,
Schild-Poulter C, Bogdanove AJ, Edgell DR. The I-TevI nuclease and linker domains
contribute to the specificity of monomeric TALENs. G3 (Bethesda). 2014 Apr
16;4(6):1155-65. doi: 10.1534/g3.114.011445. PubMed PMID: 24739648; PubMed
Central PMCID: PMC4065259.

Kleinstiver BP, Wolfs JM, Edgell DR. The monomeric GIY-YIG homing
endonuclease I-BmoI uses a molecular anchor and a flexible tether to sequentially
nick DNA. Nucleic Acids Res. 2013 May 1;41(10):5413-27. doi: 10.1093/nar/gkt186.
Epub 2013 Apr 4. PubMed PMID: 23558745; PubMed Central PMCID: PMC3664794.

Kleinstiver BP, Wolfs JM, Kolaczyk T, Roberts AK, Hu SX, Edgell DR. Monomeric
site-specific nucleases for genome editing. Proc Natl Acad Sci U S A. 2012 May
22;109(21):8061-6. doi: 10.1073/pnas.1117984109. Epub 2012 May 7. PubMed PMID:
22566637; PubMed Central PMCID: PMC3361397.
1 Protocol published
Delivery of the Cas9 or TevCas9 System into Phaeodactylum tricornutum via Conjugation of Plasmids from a Bacterial Donor
Authors:  Helen Wang, Samuel S. Slattery, Bogumil J. Karas and David R. Edgell, date: 08/20/2018, view: 630, Q&A: 0
Diatoms are an ecologically important group of eukaryotic microalgae with properties that make them attractive for biotechnological applications such as biofuels, foods, cosmetics and pharmaceuticals. Phaeodactylum tricornutum is a model ...
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