Isolation of Nuclei in Tagged Cell Types (INTACT), RNA Extraction and Ribosomal RNA Degradation to Prepare Material for RNA-Seq
Authors: Mauricio A. Reynoso,
Germain C. Pauluzzi,
Sean Cabanlit,
Joel Velasco,
Jérémie Bazin,
Roger Deal,
Siobhan Brady,
Neelima Sinha,
Julia Bailey-Serres and
Kaisa Kajala,
date: 04/05/2018,
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Gene expression is dynamically regulated on many levels, including chromatin accessibility and transcription. In order to study these nuclear regulatory events, we describe our method to purify nuclei with Isolation of Nuclei in TAgged Cell Types ...