Marco Pirazzini Department of Biomedical Sciences, University of Padova, Italy, Italy,
1 protocol

Samuele Negro Department of Biomedical Sciences, University of Padova, Italy, Italy,
1 protocol

Giulia Zanetti Department of Biomedical Sciences, University of Padova, Italy, Italy,
1 protocol

Paola Caccin
  • Department of Biomedical Sciences, University of Padova, Italy, Italy,
  • 1 Author merit


PhD, cellular biology, University of Padua, Italy, 2009

Current position

Technician, Department of Biomedical Sciences, University of Padua, Italy


  1. Caccin, P., Scorzeto, M., Damiano, N., Marin, O., Megighian, A. and Montecucco, C. (2015). The synaptotagmin juxtamembrane domain is involved in neuroexocytosis. FEBS Open Bio 5: 388-396.

  2. Bercsenyi, K., Schmieg, N., Bryson, J. B., Wallace, M., Caccin, P., Golding, M., Zanotti, G., Greensmith, L., Nischt, R. and Schiavo, G. (2014). Tetanus toxin entry. Nidogens are therapeutic targets for the prevention of tetanus. Science 346(6213): 1118-1123.

  3. Vono, M., Taccone, M., Caccin, P., Gallotta, M., Donvito, G., Falzoni, S., Palmieri, E., Pallaoro, M., Rappuoli, R., Di Virgilio, F., De Gregorio, E., Montecucco, C. and Seubert, A. (2013). The adjuvant MF59 induces ATP release from muscle that potentiates response to vaccination. Proc Natl Acad Sci U S A 110(52): 21095-21100.

  4. Fernandez, J., Caccin, P., Koster, G., Lomonte, B., Gutierrez, J. M., Montecucco, C. and Postle, A. D. (2013). Muscle phospholipid hydrolysis by Bothrops asper Asp49 and Lys49 phospholipase A(2) myotoxins--distinct mechanisms of action. FEBS J 280(16): 3878-3886.

  5. Caccin, P., Pellegatti, P., Fernandez, J., Vono, M., Cintra-Francischinelli, M., Lomonte, B., Gutierrez, J. M., Di Virgilio, F. and Montecucco, C. (2013). Why myotoxin-containing snake venoms possess powerful nucleotidases? Biochem Biophys Res Commun 430(4): 1289-1293.

  6. Eleopra, R., Montecucco, C., Devigili, G., Lettieri, C., Rinaldo, S., Verriello, L., Pirazzini, M., Caccin, P. and Rossetto, O. (2013). Botulinum neurotoxin serotype D is poorly effective in humans: an in vivo electrophysiological study. Clin Neurophysiol 124(5): 999-1004.

  7. Zornetta, I., Caccin, P., Fernandez, J., Lomonte, B., Gutierrez, J. M. and Montecucco, C. (2012). Envenomations by Bothrops and Crotalus snakes induce the release of mitochondrial alarmins. PLoS Negl Trop Dis 6(2): e1526.

  8. Silva-Cardoso, L., Caccin, P., Magnabosco, A., Patron, M., Targino, M., Fuly, A., Oliveira, G. A., Pereira, M. H., do Carmo, M., Souza, A. S., Silva-Neto, M. A., Montecucco, C. and Atella, G. C. (2010). Paralytic activity of lysophosphatidylcholine from saliva of the waterbug Belostoma anurum. J Exp Biol 213(Pt 19): 3305-3310.

  9. Cintra-Francischinelli, M., Caccin, P., Chiavegato, A., Pizzo, P., Carmignoto, G., Angulo, Y., Lomonte, B., Gutierrez, J. M. and Montecucco, C. (2010). Bothrops snake myotoxins induce a large efflux of ATP and potassium with spreading of cell damage and pain. Proc Natl Acad Sci U S A 107(32): 14140-14145.

  10. Caccin, P., Magnabosco, A., Tedesco, E., Silva-Cardoso, L., Atella, G. C. and Montecucco, C. (2010). Lysophospholipids are evolutionary ancient venom components. Toxicon 56(8): 1525-1527.

  11. Tedesco, E., Rigoni, M., Caccin, P., Grishin, E., Rossetto, O. and Montecucco, C. (2009). Calcium overload in nerve terminals of cultured neurons intoxicated by alpha-latrotoxin and snake PLA2 neurotoxins. Toxicon 54(2): 138-144.

  12. Caccin, P., Rossetto, O. and Montecucco, C. (2009). Neurotoxicity of inverted-cone shaped lipids. Neurotoxicology 30(2): 174-181.

  13. Montecucco, C., Rossetto, O., Caccin, P., Rigoni, M., Carli, L., Morbiato, L., Muraro, L. and Paoli, M. (2009). Different mechanisms of inhibition of nerve terminals by botulinum and snake presynaptic neurotoxins. Toxicon 54(5): 561-564.

  14. Rigoni, M., Paoli, M., Milanesi, E., Caccin, P., Rasola, A., Bernardi, P. and Montecucco, C. (2008). Snake phospholipase A2 neurotoxins enter neurons, bind specifically to mitochondria, and open their transition pores. J Biol Chem 283(49): 34013-34020.

  15. Rigoni, M., Pizzo, P., Schiavo, G., Weston, A. E., Zatti, G., Caccin, P., Rossetto, O., Pozzan, T. and Montecucco, C. (2007). Calcium influx and mitochondrial alterations at synapses exposed to snake neurotoxins or their phospholipid hydrolysis products. J Biol Chem 282(15): 11238-11245.

  16. Megighian, A., Rigoni, M., Caccin, P., Zordan, M. A. and Montecucco, C. (2007). A lysolecithin/fatty acid mixture promotes and then blocks neurotransmitter release at the Drosophila melanogaster larval neuromuscular junction. Neurosci Lett 416(1): 6-11.

  17. Verderio, C., Grumelli, C., Raiteri, L., Coco, S., Paluzzi, S., Caccin, P., Rossetto, O., Bonanno, G., Montecucco, C. and Matteoli, M. (2007). Traffic of botulinum toxins A and E in excitatory and inhibitory neurons. Traffic 8(2): 142-153.

  18. Caccin, P., Rigoni, M., Bisceglie, A., Rossetto, O. and Montecucco, C. (2006). Reversible skeletal neuromuscular paralysis induced by different lysophospholipids. FEBS Lett 580(27): 6317-6321.

  19. Rigoni, M., Caccin, P., Gschmeissner, S., Koster, G., Postle, A. D., Rossetto, O., Schiavo, G. and Montecucco, C. (2005). Equivalent effects of snake PLA2 neurotoxins and lysophospholipid-fatty acid mixtures. Science 310(5754): 1678-1680.

  20. Caccin, P., Rossetto, O., Rigoni, M., Johnson, E., Schiavo, G. and Montecucco, C. (2003). VAMP/synaptobrevin cleavage by tetanus and botulinum neurotoxins is strongly enhanced by acidic liposomes. FEBS Lett 542(1-3): 132-136.

  21. Rossetto, O., Caccin, P., Rigoni, M., Tonello, F., Bortoletto, N., Stevens, R. C. and Montecucco, C. (2001). Active-site mutagenesis of tetanus neurotoxin implicates TYR-375 and GLU-271 in metalloproteolytic activity. Toxicon 39(8): 1151-1159.

1 Protocol published
Mouse Phrenic Nerve Hemidiaphragm Assay (MPN)
Authors:  Giulia Zanetti, Samuele Negro, Marco Pirazzini and Paola Caccin, date: 03/05/2018, view: 255, Q&A: 0
The neuromuscular junction (NMJ) is the specialized synapse by which peripheral motor neurons innervate muscle fibers and control skeletal muscle contraction. The NMJ is the target of several xenobiotics, including chemicals, plant, animal and ...