Alba Blesa Universidad Francisco de Vitoria
26 protocols

Ana Almeida University College Cork
8 protocols

Anca Savulescu University of Cape Town
23 protocols

Anna Zorina Russian Academy of Sciences
5 protocols

Editor
Juan Facundo Rodriguez Ayala
  • Research associate, Instituto de Nanociencia y Nanotecnología
Research focus
  • Microbiology
  • Molecular mechanims involved in exoyctosis
    C. elegans, aging, B. subtilis, Probiotic, Neurobiology
    Mesoporous thin films, biodegradable polymers, osteointegration
  • 0 Author merit

Education

PhD, PROBIOL, National University of Cuyo, 2012

Lab information

Instituto de Nanociencia y Nanotecnología
Comisión Nacional de Energía Atómica (CNEA)
Av. Gral. Paz 1499 , San Martín (CP 1650),
Provincia de Buenos Aires, Argentina
http://www.cab.cnea.gov.ar/inn/index.php/el-inn

Publications

https://bio-protocol.org/e2379
1) Facundo Rodriguez Ayala, Carlos Bauman, Cecilia Leñini, Marco Bartolini, Sebastián Cogliati, Federico Argañaraz, and Roberto Grau (2017) “Induction of the host complement system by probiotic Bacillus subtilis”. Molecular Microbiology (sent).
2) Sebastián Cogliati, Facundo Rodriguez Ayala*, Carlos Bauman, Cecilia Leñini, Marco Bartolini, Juan Manuel Villalba, Federico Argañaraz and Roberto Grau (2017). “Determination of NO and CSF Levels Produced by Bacillus subtilis”. BioProtocol Vol 7, Iss 13, doi:10.21769/BioProtoc.2379 (Corresponding author).
3) Facundo Rodriguez Ayala*, Sebastián Cogliati, Carlos Bauman, Cecilia Leñini, Marco Bartolini, Juan Manuel Villalba, Federico Argañaraz and Roberto Grau (2017). “Culturing Bacteria from Caenorhabditis elegans Gut to Assess Colonization Proficiency”. BioProtocol Vol 7, Iss 12, Jun 20, doi:10.21769/BioProtoc.2345 (Corresponding author).
4) Ayala FR, Bauman C, Cogliati S, Leñini C, Bartolini M, Grau R (2017). “Microbial flora, probiotics, Bacillus subtilis and the search for a long and healthy human longevity.” Microb Cell. 16;4(4):133-136. doi: 10.15698/mic2017.04.569.
5) Rodriguez Ayala F, Bauman C, Bartolini M, Saball E, Salvarrey M, Leñini C, Cogliati S, Strauch M, Grau R. (2017). “Transcriptional regulation of adhesive properties of Bacillus subtilis to extracellular matrix proteins through the fibronectin-binding protein YloA”. Mol Microbiol. 104(5):804-821. doi: 10.1111/mmi.13666.
6) Facundo Rodriguez Ayala*; Verónica Donato*; Sebastián Cogliati*; Juan Gabriel Costa; Cecilia Leñini and Roberto Grau (2017). “Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway.” Nat Commun. 30; 8:14332. https://www.nature.com/articles/ncomms14332
7) Sebastián Cogliati; Juan Gabriel Costa; Facundo Rodriguez Ayala; Verónica Donato and Roberto Grau (2016). “Bacterial spores and its relatives as agents of mass destruction”. J Bioterror Biodef, 7:1. http://dx.doi.org/10.4172/2157-2526.1000141.
8) Rodriguez, F; Zanetti, MN; Mayorga LS; Tomes CN (2012). ˮMunc18 controls protein complex assembly during human sperm acrosomal exocytosis”. J Biol Chem. 21; 287(52):43825-39; doi: 10.1074/jbc.M112.409649.
9) Rodriguez, F; Bustos, MA; Zanetti, MN; Ruete, C; Mayorga LS; Tomes CN (2011). ˮα-SNAP prevents docking of the acrosome because sequesters monomeric syntaxin” PLoS One; 6(7):e21925; doi: 10.1371/journal.pone.0021925.
10) Bátiz LF, De BlasGA, Michaut MA, Ramírez AR, Rodriguez F, Ratto MH, Oliver C, Tomes CN, Rodríguez EM, and Mayorga LS (2009). ˮA point mutation of aSNAP causes impaired acrosome reaction and reduced male fertilityˮ. PLoS ONE. 4(3):e4963; doi: 10.1371/journal.pone.0004963.
9 Protocols reviewed
Multiple Modification of Chromosomal Loci Using URA5.3 Selection Marker in the Unicellular Red Alga Cyanidioschyzon merolae
Authors:  Tokiaki Takemura, Sousuke Imamura, Yuki Kobayashi and Kan Tanaka, date: 04/05/2019, view: 3544, Q&A: 0
The unicellular red alga Cyanidioschyzon merolae has been used as a eukaryotic photosynthetic model for various basic and applied studies. Although the nuclear genome of C. merolae can be modified by homologous recombination with ...
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Microirradiation for Precise, Double-strand Break Induction in vivo in Caenorhabditis elegans
Authors:  Kailey E. Harrell, Emily Koury and Sarit Smolikove, date: 12/20/2018, view: 3167, Q&A: 0
DNA double-strand breaks (DSBs) are toxic lesions that every cell must accurately repair in order to survive. The repair of DSBs is an integral part of a cell life cycle and can lead to lethality if repaired incorrectly. Laser microirradiation is an ...
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16 Protocols edited
Electron Tomography to Study the Three-dimensional Structure of the Reovirus Egress Pathway in Mammalian Cells

Mammalian orthoreoviruses (reoviruses) are nonenveloped, double-stranded RNA viruses that replicate and assemble in cytoplasmic membranous organelles called viral inclusions (VIs). To define the cellular compartments involved in nonlytic reovirus

...
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Tracking the Subcellular Localization of Surface Proteins in Staphylococcus aureus by Immunofluorescence Microscopy
Authors:  Salvatore J. Scaffidi, Mac A. Shebes and Wenqi Yu, date: 05/20/2021, view: 3022, Q&A: 0

Surface proteins of Staphylococcus aureus and other Gram-positive bacteria play essential roles in bacterial colonization and host-microbe interactions. Surface protein precursors containing a YSIRK/GXXS signal peptide are translocated across the

...
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