Anna Zorina Russian Academy of Sciences
4 protocols

Chao Jiang Zhejiang University
5 protocols

David Cisneros Umeå University
28 protocols

Dennis Nürnberg Imperial College London
47 protocols

Editor
Juan Facundo Rodriguez Ayala
  • Research associate, Instituto de Nanociencia y Nanotecnología
Research focus
  • Microbiology
  • Molecular mechanims involved in exoyctosis
    C. elegans, aging, B. subtilis, Probiotic, Neurobiology
    Mesoporous thin films, biodegradable polymers, osteointegration

Education

PhD, PROBIOL, National University of Cuyo, 2012

Lab information

Instituto de Nanociencia y Nanotecnología
Comisión Nacional de Energía Atómica (CNEA)
Av. Gral. Paz 1499 , San Martín (CP 1650),
Provincia de Buenos Aires, Argentina
http://www.cab.cnea.gov.ar/inn/index.php/el-inn

Publications

https://bio-protocol.org/e2379
1) Facundo Rodriguez Ayala, Carlos Bauman, Cecilia Leñini, Marco Bartolini, Sebastián Cogliati, Federico Argañaraz, and Roberto Grau (2017) “Induction of the host complement system by probiotic Bacillus subtilis”. Molecular Microbiology (sent).
2) Sebastián Cogliati, Facundo Rodriguez Ayala*, Carlos Bauman, Cecilia Leñini, Marco Bartolini, Juan Manuel Villalba, Federico Argañaraz and Roberto Grau (2017). “Determination of NO and CSF Levels Produced by Bacillus subtilis”. BioProtocol Vol 7, Iss 13, doi:10.21769/BioProtoc.2379 (Corresponding author).
3) Facundo Rodriguez Ayala*, Sebastián Cogliati, Carlos Bauman, Cecilia Leñini, Marco Bartolini, Juan Manuel Villalba, Federico Argañaraz and Roberto Grau (2017). “Culturing Bacteria from Caenorhabditis elegans Gut to Assess Colonization Proficiency”. BioProtocol Vol 7, Iss 12, Jun 20, doi:10.21769/BioProtoc.2345 (Corresponding author).
4) Ayala FR, Bauman C, Cogliati S, Leñini C, Bartolini M, Grau R (2017). “Microbial flora, probiotics, Bacillus subtilis and the search for a long and healthy human longevity.” Microb Cell. 16;4(4):133-136. doi: 10.15698/mic2017.04.569.
5) Rodriguez Ayala F, Bauman C, Bartolini M, Saball E, Salvarrey M, Leñini C, Cogliati S, Strauch M, Grau R. (2017). “Transcriptional regulation of adhesive properties of Bacillus subtilis to extracellular matrix proteins through the fibronectin-binding protein YloA”. Mol Microbiol. 104(5):804-821. doi: 10.1111/mmi.13666.
6) Facundo Rodriguez Ayala*; Verónica Donato*; Sebastián Cogliati*; Juan Gabriel Costa; Cecilia Leñini and Roberto Grau (2017). “Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway.” Nat Commun. 30; 8:14332. https://www.nature.com/articles/ncomms14332
7) Sebastián Cogliati; Juan Gabriel Costa; Facundo Rodriguez Ayala; Verónica Donato and Roberto Grau (2016). “Bacterial spores and its relatives as agents of mass destruction”. J Bioterror Biodef, 7:1. http://dx.doi.org/10.4172/2157-2526.1000141.
8) Rodriguez, F; Zanetti, MN; Mayorga LS; Tomes CN (2012). ˮMunc18 controls protein complex assembly during human sperm acrosomal exocytosis”. J Biol Chem. 21; 287(52):43825-39; doi: 10.1074/jbc.M112.409649.
9) Rodriguez, F; Bustos, MA; Zanetti, MN; Ruete, C; Mayorga LS; Tomes CN (2011). ˮα-SNAP prevents docking of the acrosome because sequesters monomeric syntaxin” PLoS One; 6(7):e21925; doi: 10.1371/journal.pone.0021925.
10) Bátiz LF, De BlasGA, Michaut MA, Ramírez AR, Rodriguez F, Ratto MH, Oliver C, Tomes CN, Rodríguez EM, and Mayorga LS (2009). ˮA point mutation of aSNAP causes impaired acrosome reaction and reduced male fertilityˮ. PLoS ONE. 4(3):e4963; doi: 10.1371/journal.pone.0004963.
9 Protocols reviewed
Multiple Modification of Chromosomal Loci Using URA5.3 Selection Marker in the Unicellular Red Alga Cyanidioschyzon merolae
Authors:  Tokiaki Takemura, Sousuke Imamura, Yuki Kobayashi and Kan Tanaka, date: 04/05/2019, view: 1468, Q&A: 0
The unicellular red alga Cyanidioschyzon merolae has been used as a eukaryotic photosynthetic model for various basic and applied studies. Although the nuclear genome of C. merolae can be modified by homologous recombination with ...
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Microirradiation for Precise, Double-strand Break Induction in vivo in Caenorhabditis elegans
Authors:  Kailey E. Harrell, Emily Koury and Sarit Smolikove, date: 12/20/2018, view: 1910, Q&A: 0
DNA double-strand breaks (DSBs) are toxic lesions that every cell must accurately repair in order to survive. The repair of DSBs is an integral part of a cell life cycle and can lead to lethality if repaired incorrectly. Laser microirradiation is an ...
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6 Protocols edited
Measurement of the Promoter Activity in Escherichia coli by Using a Luciferase Reporter
Authors:  Yuki Yamanaka, Hiroki Watanabe, Erika Yamauchi, Yukari Miyake and Kaneyoshi Yamamoto, date: 01/20/2020, view: 278, Q&A: 0
The reporter system is widely used technique for measuring promoter activity in bacterial cells. Until now, a number of reporter system have been developed, but the bioluminescent reporter constructed from the bacterial luciferase genes is one of ...
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Microfluidic-based Growth and Imaging of Bacterial Biofilms
Authors:  James M Brown, Birte Blunk, Paul Williams and Kim R Hardie, date: 12/20/2019, view: 347, Q&A: 0
Biofilms are a ubiquitous form of growth for bacteria on surfaces in most environments, natural or manmade. Here we present a protocol using the Bioflux microfluidic system to investigate the organized structure and development of these ...
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