Anna Zorina Russian Academy of Sciences
4 protocols

David Cisneros Umeå University
25 protocols

Dennis Nürnberg Imperial College London
46 protocols

DURAI SELLEGOUNDER Washington State University
2 protocols

Editor
Juan Facundo Rodriguez Ayala
  • Research associate, National University of Rosario
Research focus
  • Microbiology
  • C. elegans, aging, B. subtilis, Probiotic, Neurobiology

Education

PhD, PROBIOL, National University of Cuyo, 2012

Lab information

Dr. Roberto Grau's lab
http://microbiologiarosario.org/

Publications

https://bio-protocol.org/e2379
1) Facundo Rodriguez Ayala, Carlos Bauman, Cecilia Leñini, Marco Bartolini, Sebastián Cogliati, Federico Argañaraz, and Roberto Grau (2017) “Induction of the host complement system by probiotic Bacillus subtilis”. Molecular Microbiology (sent).
2) Sebastián Cogliati, Facundo Rodriguez Ayala*, Carlos Bauman, Cecilia Leñini, Marco Bartolini, Juan Manuel Villalba, Federico Argañaraz and Roberto Grau (2017). “Determination of NO and CSF Levels Produced by Bacillus subtilis”. BioProtocol Vol 7, Iss 13, doi:10.21769/BioProtoc.2379 (Corresponding author).
3) Facundo Rodriguez Ayala*, Sebastián Cogliati, Carlos Bauman, Cecilia Leñini, Marco Bartolini, Juan Manuel Villalba, Federico Argañaraz and Roberto Grau (2017). “Culturing Bacteria from Caenorhabditis elegans Gut to Assess Colonization Proficiency”. BioProtocol Vol 7, Iss 12, Jun 20, doi:10.21769/BioProtoc.2345 (Corresponding author).
4) Ayala FR, Bauman C, Cogliati S, Leñini C, Bartolini M, Grau R (2017). “Microbial flora, probiotics, Bacillus subtilis and the search for a long and healthy human longevity.” Microb Cell. 16;4(4):133-136. doi: 10.15698/mic2017.04.569.
5) Rodriguez Ayala F, Bauman C, Bartolini M, Saball E, Salvarrey M, Leñini C, Cogliati S, Strauch M, Grau R. (2017). “Transcriptional regulation of adhesive properties of Bacillus subtilis to extracellular matrix proteins through the fibronectin-binding protein YloA”. Mol Microbiol. 104(5):804-821. doi: 10.1111/mmi.13666.
6) Facundo Rodriguez Ayala*; Verónica Donato*; Sebastián Cogliati*; Juan Gabriel Costa; Cecilia Leñini and Roberto Grau (2017). “Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway.” Nat Commun. 30; 8:14332. https://www.nature.com/articles/ncomms14332
7) Sebastián Cogliati; Juan Gabriel Costa; Facundo Rodriguez Ayala; Verónica Donato and Roberto Grau (2016). “Bacterial spores and its relatives as agents of mass destruction”. J Bioterror Biodef, 7:1. http://dx.doi.org/10.4172/2157-2526.1000141.
8) Rodriguez, F; Zanetti, MN; Mayorga LS; Tomes CN (2012). ˮMunc18 controls protein complex assembly during human sperm acrosomal exocytosis”. J Biol Chem. 21; 287(52):43825-39; doi: 10.1074/jbc.M112.409649.
9) Rodriguez, F; Bustos, MA; Zanetti, MN; Ruete, C; Mayorga LS; Tomes CN (2011). ˮα-SNAP prevents docking of the acrosome because sequesters monomeric syntaxin” PLoS One; 6(7):e21925; doi: 10.1371/journal.pone.0021925.
10) Bátiz LF, De BlasGA, Michaut MA, Ramírez AR, Rodriguez F, Ratto MH, Oliver C, Tomes CN, Rodríguez EM, and Mayorga LS (2009). ˮA point mutation of aSNAP causes impaired acrosome reaction and reduced male fertilityˮ. PLoS ONE. 4(3):e4963; doi: 10.1371/journal.pone.0004963.
9 Protocols reviewed
Multiple Modification of Chromosomal Loci Using URA5.3 Selection Marker in the Unicellular Red Alga Cyanidioschyzon merolae
Authors:  Tokiaki Takemura, Sousuke Imamura, Yuki Kobayashi and Kan Tanaka, date: 04/05/2019, view: 1020, Q&A: 0
The unicellular red alga Cyanidioschyzon merolae has been used as a eukaryotic photosynthetic model for various basic and applied studies. Although the nuclear genome of C. merolae can be modified by homologous recombination with ...
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Microirradiation for Precise, Double-strand Break Induction in vivo in Caenorhabditis elegans
Authors:  Kailey E. Harrell, Emily Koury and Sarit Smolikove, date: 12/20/2018, view: 1564, Q&A: 0
DNA double-strand breaks (DSBs) are toxic lesions that every cell must accurately repair in order to survive. The repair of DSBs is an integral part of a cell life cycle and can lead to lethality if repaired incorrectly. Laser microirradiation is an ...
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2 Protocols edited
Analyzing the Functionality of Non-native Hsp70 Proteins in Saccharomyces cerevisiae
Authors:  Laura E. Knighton, Lizbeth P. Saa, Adam M. Reitzel and Andrew W. Truman, date: 10/05/2019, view: 108, Q&A: 0
Yeast are an ideal system to study Heat Shock Protein 70 (Hsp70) function in a cellular context. This protocol was generated to analyze the function of non-native Hsp70 proteins by expressing them as the sole cytosolic Hsp70 in yeast. As an initial ...
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Imaging Gene Expression Dynamics in Pseudomonas fluorescens In5 during Interactions with the Fungus Fusarium graminearum PH-1
Authors:  Rosanna C. Hennessy, Peter Stougaard and Stefan Olsson, date: 06/20/2019, view: 810, Q&A: 0
Genomics, transcriptomics and metabolomics are powerful technologies for studying microbial interactions. The main drawback of these methods is the requirement for destructive sampling. We have established an alternative but complementary technique ...
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