Alba Blesa Universidad Francisco de Vitoria
18 protocols

Anna Zorina Russian Academy of Sciences
5 protocols

Chao Jiang Zhejiang University
8 protocols

David Cisneros Umeå University
31 protocols

Juan Facundo Rodriguez Ayala
  • Research associate, Instituto de Nanociencia y Nanotecnología
Research focus
  • Microbiology
  • Molecular mechanims involved in exoyctosis
    C. elegans, aging, B. subtilis, Probiotic, Neurobiology
    Mesoporous thin films, biodegradable polymers, osteointegration
  • 0 Author merit


PhD, PROBIOL, National University of Cuyo, 2012

Lab information

Instituto de Nanociencia y Nanotecnología
Comisión Nacional de Energía Atómica (CNEA)
Av. Gral. Paz 1499 , San Martín (CP 1650),
Provincia de Buenos Aires, Argentina

1) Facundo Rodriguez Ayala, Carlos Bauman, Cecilia Leñini, Marco Bartolini, Sebastián Cogliati, Federico Argañaraz, and Roberto Grau (2017) “Induction of the host complement system by probiotic Bacillus subtilis”. Molecular Microbiology (sent).
2) Sebastián Cogliati, Facundo Rodriguez Ayala*, Carlos Bauman, Cecilia Leñini, Marco Bartolini, Juan Manuel Villalba, Federico Argañaraz and Roberto Grau (2017). “Determination of NO and CSF Levels Produced by Bacillus subtilis”. BioProtocol Vol 7, Iss 13, doi:10.21769/BioProtoc.2379 (Corresponding author).
3) Facundo Rodriguez Ayala*, Sebastián Cogliati, Carlos Bauman, Cecilia Leñini, Marco Bartolini, Juan Manuel Villalba, Federico Argañaraz and Roberto Grau (2017). “Culturing Bacteria from Caenorhabditis elegans Gut to Assess Colonization Proficiency”. BioProtocol Vol 7, Iss 12, Jun 20, doi:10.21769/BioProtoc.2345 (Corresponding author).
4) Ayala FR, Bauman C, Cogliati S, Leñini C, Bartolini M, Grau R (2017). “Microbial flora, probiotics, Bacillus subtilis and the search for a long and healthy human longevity.” Microb Cell. 16;4(4):133-136. doi: 10.15698/mic2017.04.569.
5) Rodriguez Ayala F, Bauman C, Bartolini M, Saball E, Salvarrey M, Leñini C, Cogliati S, Strauch M, Grau R. (2017). “Transcriptional regulation of adhesive properties of Bacillus subtilis to extracellular matrix proteins through the fibronectin-binding protein YloA”. Mol Microbiol. 104(5):804-821. doi: 10.1111/mmi.13666.
6) Facundo Rodriguez Ayala*; Verónica Donato*; Sebastián Cogliati*; Juan Gabriel Costa; Cecilia Leñini and Roberto Grau (2017). “Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway.” Nat Commun. 30; 8:14332.
7) Sebastián Cogliati; Juan Gabriel Costa; Facundo Rodriguez Ayala; Verónica Donato and Roberto Grau (2016). “Bacterial spores and its relatives as agents of mass destruction”. J Bioterror Biodef, 7:1.
8) Rodriguez, F; Zanetti, MN; Mayorga LS; Tomes CN (2012). ˮMunc18 controls protein complex assembly during human sperm acrosomal exocytosis”. J Biol Chem. 21; 287(52):43825-39; doi: 10.1074/jbc.M112.409649.
9) Rodriguez, F; Bustos, MA; Zanetti, MN; Ruete, C; Mayorga LS; Tomes CN (2011). ˮα-SNAP prevents docking of the acrosome because sequesters monomeric syntaxin” PLoS One; 6(7):e21925; doi: 10.1371/journal.pone.0021925.
10) Bátiz LF, De BlasGA, Michaut MA, Ramírez AR, Rodriguez F, Ratto MH, Oliver C, Tomes CN, Rodríguez EM, and Mayorga LS (2009). ˮA point mutation of aSNAP causes impaired acrosome reaction and reduced male fertilityˮ. PLoS ONE. 4(3):e4963; doi: 10.1371/journal.pone.0004963.
9 Protocols reviewed
Multiple Modification of Chromosomal Loci Using URA5.3 Selection Marker in the Unicellular Red Alga Cyanidioschyzon merolae
Authors:  Tokiaki Takemura, Sousuke Imamura, Yuki Kobayashi and Kan Tanaka, date: 04/05/2019, view: 2639, Q&A: 0
The unicellular red alga Cyanidioschyzon merolae has been used as a eukaryotic photosynthetic model for various basic and applied studies. Although the nuclear genome of C. merolae can be modified by homologous recombination with ...
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Microirradiation for Precise, Double-strand Break Induction in vivo in Caenorhabditis elegans
Authors:  Kailey E. Harrell, Emily Koury and Sarit Smolikove, date: 12/20/2018, view: 2574, Q&A: 0
DNA double-strand breaks (DSBs) are toxic lesions that every cell must accurately repair in order to survive. The repair of DSBs is an integral part of a cell life cycle and can lead to lethality if repaired incorrectly. Laser microirradiation is an ...
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11 Protocols edited
Evaluation of Viable Cells in Pseudomonas aeruginosa Biofilms by Colony Count and Live/Dead Staining
Authors:  Magdalena Pezzoni, Ramón Augusto Pizarro and Cristina Susana Costa, date: 09/20/2020, view: 366, Q&A: 0
Pseudomonas aeruginosa is a human pathogen capable to form robust biofilms. P. aeruginosa biofilms represent a serious problem because of the adverse effects on human health and industry, from sanitary and economic points of view. ...
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Quantification of Bacteria Residing in Caenorhabditis elegans Intestine
Authors:  M. Fernanda Palominos and Andrea Calixto, date: 05/05/2020, view: 1250, Q&A: 0
Quantification of intestinal colonization by pathogenic or commensal bacteria constitute a critical part of the analysis to understand host-microbe interactions during different time points of their interplay. Here we detail a method to isolate ...
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