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Coauthors
Carlos Duarte CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Portugal, Portugal,
2 protocols

Simone Frisari CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Portugal, Portugal,
1 protocol

Bárbara Correia CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Portugal, Portugal,
1 protocol

Rui Costa CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Portugal, Portugal,
1 protocol

Miranda Mele
  • CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Portugal, Portugal,
  • 1 Author merit

Education

PhD, University of Coimbra , Portugal, 2013

Current position

Post-Doc, Neuroscience and disease, Center for Neuroscience and Cell Biology of Coimbra, Portugal

Publications

  1. Mele, M., Leal, G. and Duarte, C. B. (2016). Role of GABAA R trafficking in the plasticity of inhibitory synapses. J Neurochem 139(6): 997-1018.
  2. Curcio, M., Salazar, I. L., Mele, M., Canzoniero, L. M. and Duarte, C. B. (2016). Calpains and neuronal damage in the ischemic brain: The swiss knife in synaptic injury. Prog Neurobiol 143: 1-35.
  3. Mele, M., Aspromonte, M. C. and Duarte, C. B. (2017). Downregulation of GABAA Receptor Recycling Mediated by HAP1 Contributes to Neuronal Death in In Vitro Brain Ischemia. Mol Neurobiol 54(1): 45-57.
  4. Vieira, M. M., Schmidt, J., Ferreira, J. S., She, K., Oku, S., Mele, M., Santos, A. E., Duarte, C. B., Craig, A. M. and Carvalho, A. L. (2016). Multiple domains in the C-terminus of NMDA receptor GluN2B subunit contribute to neuronal death following in vitro ischemia. Neurobiol Dis 89: 223-234.
  5. Costa, J. T., Mele, M., Baptista, M. S., Gomes, J. R., Ruscher, K., Nobre, R. J., de Almeida, L. P., Wieloch, T. and Duarte, C. B. (2016). Gephyrin Cleavage in In Vitro Brain Ischemia Decreases GABAA Receptor Clustering and Contributes to Neuronal Death. Mol Neurobiol 53(6): 3513-3527.
  6. Santos, A. R., Mele, M., Vaz, S. H., Kellermayer, B., Grimaldi, M., Colino-Oliveira, M., Rombo, D. M., Comprido, D., Sebastiao, A. M. and Duarte, C. B. (2015). Differential role of the proteasome in the early and late phases of BDNF-induced facilitation of LTP. J Neurosci 35(8): 3319-3329.
  7. Mele, M., Ribeiro, L., Inacio, A. R., Wieloch, T. and Duarte, C. B. (2014). GABA(A) receptor dephosphorylation followed by internalization is coupled to neuronal death in in vitro ischemia. Neurobiol Dis 65: 220-232.
  8. Melo, C. V., Mele, M., Curcio, M., Comprido, D., Silva, C. G. and Duarte, C. B. (2013). BDNF regulates the expression and distribution of vesicular glutamate transporters in cultured hippocampal neurons. PLoS One 8(1): e53793.
  9. Caldeira, M. V., Curcio, M., Leal, G., Salazar, I. L., Mele, M., Santos, A. R., Melo, C. V., Pereira, P., Canzoniero, L. M. and Duarte, C. B. (2013). Excitotoxic stimulation downregulates the ubiquitin-proteasome system through activation of NMDA receptors in cultured hippocampal neurons. Biochim Biophys Acta 1832(1): 263-274.
  10. Lobo, A. C., Gomes, J. R., Catarino, T., Mele, M., Fernandez, P., Inacio, A. R., Bahr, B. A., Santos, A. E., Wieloch, T., Carvalho, A. L. and Duarte, C. B. (2011). Cleavage of the vesicular glutamate transporters under excitotoxic conditions. Neurobiol Dis 44(3): 292-303.
1 Protocol published
Preparation of Primary Cultures of Embryonic Rat Hippocampal and Cerebrocortical Neurons
This protocol aims at standardizing the procedure to obtain primary cultures of hippocampal and cerebrocortical neurons for in vitro experiments. Cultures should be prepared from cells isolated during embryonic development when neuronal ...