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Coauthors
Toshimitsu Kawate Molecular Medicine, Cornell University, USA, USA,
2 protocols
Coreviewers
Ralph Boettcher Max Planck Institute for Biochemistry
31 protocols

Sarah Diermeier University of Otago
1 protocol

Reviewer
Akira Karasawa
  • Molecular Medicine, Cornell University, USA
  • 2 Author merit

Education

Ph.D, Osaka University, Japan, 2008

Current position

Research associate, Molecular Medicine, Cornell University, United States

Publications

  1. Karasawa, A. and Kawate, T. (2016). Structural basis for subtype-specific inhibition of the P2X7 receptor. Elife 5.
  2. Karasawa, A., Swier, L. J., Stuart, M. C., Brouwers, J., Helms, B. and Poolman, B. (2013). Physicochemical factors controlling the activity and energy coupling of an ionic strength-gated ATP-binding cassette (ABC) transporter. J Biol Chem 288(41): 29862-29871.
  3. Gul, N., Schuurman-Wolters, G., Karasawa, A. and Poolman, B. (2012). Functional characterization of amphipathic a-helix in the osmoregulatory ABC transporter OpuA. Biochemistry 51: 5142-52.
  4. Karasawa, A., Erkens, G. B., Berntsson, R. P., Otten, R., Schuurman-Wolters, G. K., Mulder, F. A. and Poolman, B. (2011). Cystathionine beta-synthase (CBS) domains 1 and 2 fulfill different roles in ionic strength sensing of the ATP-binding cassette (ABC) transporter OpuA. J Biol Chem 286(43): 37280-37291.
  5. Wolters, J. C., Berntsson, R. P., Gul, N., Karasawa, A., Thunnissen, A. M., Slotboom, D. J. and Poolman, B. (2010). Ligand binding and crystal structures of the substrate-binding domain of the ABC transporter OpuA. PLoS One 5(4): e10361.
  6. Karasawa, A., Mitsui, K., Matsushita, M. and Kanazawa, H. (2010). Intermolecular cross-linking of monomers in Helicobacter pylori Na+/H+ antiporter NhaA at the dimer interface inhibits antiporter activity. Biochem J 426(1): 99-108.
  7. Karasawa, A., Mitsui, K., Matsushita, M. and Kanazawa, H. (2007). Functional assembly of the Na+/H+ antiporter of Helicobacter pylori from partial fragments in vivo. Biochemistry 46(49): 14272-14283.
  8. Karasawa, A., Tsuboi, Y., Inoue, H., Kinoshita, R., Nakamura, N. and Kanazawa, H. (2005). Detection of oligomerization and conformational changes in the Na+/H+ antiporter from Helicobacter pylori by fluorescence resonance energy transfer. J Biol Chem 280(51): 41900-41911.
2 Protocols published
High-throughput YO-PRO-1 Uptake Assay for P2X7 Receptors Expressed in HEK Cells
Authors:  Akira Karasawa and Toshimitsu Kawate, date: 07/20/2018, view: 173, Q&A: 0
P2X7 receptors are extracellular ATP-gated ion channels that play broad physiological and pathological roles in animals (Sluyter, 2017). Activation of P2X7 receptors lead to the opening of membrane channels permeable for small cations like Na+ ...
Expression and Purification of a Mammalian P2X7 Receptor from Sf9 Insect Cells
Authors:  Akira Karasawa and Toshimitsu Kawate, date: 09/05/2017, view: 2808, Q&A: 0
The P2X7 receptor is an extracellular ATP-gated ion channel found only in eukaryotes (Bartlett et al., 2014). Due to its unique properties among P2X receptors, such as formation of a large conductance pore, the P2X7 receptor has been ...
1 Protocol reviewed
Guanine Nucleotide Exchange Assay Using Fluorescent MANT-GDP
Authors:  Tomoharu Kanie and Peter K. Jackson, date: 04/05/2018, view: 1289, Q&A: 0
GTPases are molecular switches that cycle between the inactive GDP-bound state and the active GTP-bound state. GTPases exchange nucleotides either by its intrinsic nucleotide exchange or by interaction with guanine nucleotide exchange factors ...
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