Toshimitsu Kawate Molecular Medicine, Cornell University, USA, USA,
2 protocols
Beatrice Li University of Oxford
7 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
93 protocols

Joe Zhang Stanford University
4 protocols

Longping Tse University of North Carolina at Chapel Hill
27 protocols

Reviewer
Akira Karasawa
  • Molecular Medicine, Cornell University, USA
Research focus
  • Microbiology
  • 2 Author merit

Education

Ph.D, Osaka University, Japan, 2008

Current position

Research associate, Molecular Medicine, Cornell University, United States

Publications

  1. Karasawa, A. and Kawate, T. (2016). Structural basis for subtype-specific inhibition of the P2X7 receptor. Elife 5.
  2. Karasawa, A., Swier, L. J., Stuart, M. C., Brouwers, J., Helms, B. and Poolman, B. (2013). Physicochemical factors controlling the activity and energy coupling of an ionic strength-gated ATP-binding cassette (ABC) transporter. J Biol Chem 288(41): 29862-29871.
  3. Gul, N., Schuurman-Wolters, G., Karasawa, A. and Poolman, B. (2012). Functional characterization of amphipathic a-helix in the osmoregulatory ABC transporter OpuA. Biochemistry 51: 5142-52.
  4. Karasawa, A., Erkens, G. B., Berntsson, R. P., Otten, R., Schuurman-Wolters, G. K., Mulder, F. A. and Poolman, B. (2011). Cystathionine beta-synthase (CBS) domains 1 and 2 fulfill different roles in ionic strength sensing of the ATP-binding cassette (ABC) transporter OpuA. J Biol Chem 286(43): 37280-37291.
  5. Wolters, J. C., Berntsson, R. P., Gul, N., Karasawa, A., Thunnissen, A. M., Slotboom, D. J. and Poolman, B. (2010). Ligand binding and crystal structures of the substrate-binding domain of the ABC transporter OpuA. PLoS One 5(4): e10361.
  6. Karasawa, A., Mitsui, K., Matsushita, M. and Kanazawa, H. (2010). Intermolecular cross-linking of monomers in Helicobacter pylori Na+/H+ antiporter NhaA at the dimer interface inhibits antiporter activity. Biochem J 426(1): 99-108.
  7. Karasawa, A., Mitsui, K., Matsushita, M. and Kanazawa, H. (2007). Functional assembly of the Na+/H+ antiporter of Helicobacter pylori from partial fragments in vivo. Biochemistry 46(49): 14272-14283.
  8. Karasawa, A., Tsuboi, Y., Inoue, H., Kinoshita, R., Nakamura, N. and Kanazawa, H. (2005). Detection of oligomerization and conformational changes in the Na+/H+ antiporter from Helicobacter pylori by fluorescence resonance energy transfer. J Biol Chem 280(51): 41900-41911.
2 Protocols published
High-throughput YO-PRO-1 Uptake Assay for P2X7 Receptors Expressed in HEK Cells
Authors:  Akira Karasawa and Toshimitsu Kawate, date: 07/20/2018, view: 3707, Q&A: 0
P2X7 receptors are extracellular ATP-gated ion channels that play broad physiological and pathological roles in animals (Sluyter, 2017). Activation of P2X7 receptors lead to the opening of membrane channels permeable for small cations like Na+ ...
Expression and Purification of a Mammalian P2X7 Receptor from Sf9 Insect Cells
Authors:  Akira Karasawa and Toshimitsu Kawate, date: 09/05/2017, view: 6801, Q&A: 0
The P2X7 receptor is an extracellular ATP-gated ion channel found only in eukaryotes (Bartlett et al., 2014). Due to its unique properties among P2X receptors, such as formation of a large conductance pore, the P2X7 receptor has been ...
5 Protocols reviewed
Expression and Purification of Adeno-associated Virus Virus-like Particles in a Baculovirus System and AAVR Ectodomain Constructs in E. coli
Adeno-associated virus (AAV) is a promising gene therapy vector and the biophysical characterization of its interactions with host proteins is a critical foundation for engineering tissue targeting and immune escape. Presented here are protocols for ...
More >
Rapid Detection of Proliferative Bacteria by Electrical Stimulation
Authors:  Conor LA Edwards, Dmitry Malyshev, James P Stratford and Munehiro Asally, date: 02/05/2020, view: 1601, Q&A: 0
Detecting live bacteria is an important task for antimicrobial susceptibility testing (AST) in the medical sector and for quality-monitoring in biological industries. Current methods for live-bacteria detection suffer limitations in speed or ...
More >
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.