Toshimitsu Kawate Molecular Medicine, Cornell University, USA, USA,
2 protocols
Arif Kabir Hokkaido University
1 protocol

Beatrice Li University of Oxford
7 protocols

Carey Huh University of California
4 protocols

Chen Fan Weill Cornell Medical College
0 protocol

Akira Karasawa
  • Research scientist, New York Structural Biology Center
Research focus
  • Biophysics
  • Membrane protein biochemistry/biophysics
  • 2 Author merit


PhD in Biology, Osaka University, 2008

Lab information


1. Karasawa, A. and Kawate, T. High-throughput YO-PRO-1 Uptake Assay for P2X7 Receptors Expressed in HEK Cells.
Bio-101: e2943 (2018)

2. Karasawa, A., Michalski, K., Mikhelzon, P., and Kawate, T.
The P2X7 receptor forms a dye-permeable pore independent of its intracellular domain but dependent on membrane lipid composition.
Elife 6, e31186 (2017)

3. Karasawa, A., and Kawate, T.
Expression and Purification of a Mammalian P2X7 Receptor from Sf9 Insect Cells.
Bio-protocol 7, e2544 (2017).

4. Karasawa, A., and Kawate, T.
Structural basis for subtype-specific inhibition of the P2X7 receptor.
Elife 5, e22153 (2016)

5. Karasawa A., Swier L.J., Stuart M.C., Brouwers J., Helms B., Poolman B.
Physicochemical factors controlling the activity and energy coupling of an ionic strength-gated ATP-binding cassette (ABC) transporter.
J. Biol. Chem. 288, 29862-71 (2013)

6. Gul N., Schuurman-Wolters G., Karasawa A., Poolman B.
Functional characterization of amphipathic a-helix in the osmoregulatory ABC transporter OpuA.
Biochemistry. 51, 5142-52 (2012)

7. Karasawa A., Erkens G.B., Berntsson R.P., Otten R., Schuurman-Wolters G.K., Mulder F.A., Poolman B.
Cystathionine b synthase CBS domains 1 and 2 fulfill different roles in ionic strength-sensing of the ABC transporter OpuA.
J. Biol. Chem. 286, 37280-37291, (2011)

8. Wolters J.C., Berntsson R.P., Gul N., Karasawa A, Thunnissen A.M., Slotboom D.J., Poolman B.
Ligand binding and crystal structures of the substrate-binding domain of the ABC transporter OpuA.
PLoS One. 5: e10361 (2010)

9. Karasawa A, Mitsui K., Matsushita M., Kanazawa H.
Intermolecular cross-linking of monomers in H. pylori Na+/H+ antiporter NhaA at dimer interface inhibits antiporter activity.
Biochem J. 426, 99–108 (2010)

10. Karasawa A., Mitsui K., Matsushita M., Kanazawa H.
Functional Assembly of the Na+/H+ Antiporter of Helicobacter pylori from Partial Fragments in vivo.
Biochemistry. 46, 14272-83 (2007)

11. Karasawa A., Tsuboi Y., Inoue H., Kinoshita R., Nakamura N., Kanazawa H.
Detection of oligomerization and conformational changes in the Na+/H+ antiporter from Helicobacter pylori by fluorescence resonance energy transfer.
J. Biol. Chem. 280, 41900-41911, (2005)
2 Protocols published
High-throughput YO-PRO-1 Uptake Assay for P2X7 Receptors Expressed in HEK Cells
Authors:  Akira Karasawa and Toshimitsu Kawate, date: 07/20/2018, view: 4596, Q&A: 0
P2X7 receptors are extracellular ATP-gated ion channels that play broad physiological and pathological roles in animals (Sluyter, 2017). Activation of P2X7 receptors lead to the opening of membrane channels permeable for small cations like Na+ ...
Expression and Purification of a Mammalian P2X7 Receptor from Sf9 Insect Cells
Authors:  Akira Karasawa and Toshimitsu Kawate, date: 09/05/2017, view: 7914, Q&A: 0
The P2X7 receptor is an extracellular ATP-gated ion channel found only in eukaryotes (Bartlett et al., 2014). Due to its unique properties among P2X receptors, such as formation of a large conductance pore, the P2X7 receptor has been ...
6 Protocols reviewed
Preparing Viable Hippocampal Slices from Adult Mice for the Study of Sharp Wave-ripples
Authors:  Linhua Liu, Xiaojing Zhou and Jian-young Wu, date: 10/05/2020, view: 766, Q&A: 0
We describe a protocol for preparing acute brain slices which can produce robust hippocampal sharp wave-ripples (SWRs) in vitro. The protocol is optimized for its simplicity and reliability for the preparation of solutions, slicing, and ...
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Expression and Purification of Adeno-associated Virus Virus-like Particles in a Baculovirus System and AAVR Ectodomain Constructs in E. coli
Adeno-associated virus (AAV) is a promising gene therapy vector and the biophysical characterization of its interactions with host proteins is a critical foundation for engineering tissue targeting and immune escape. Presented here are protocols for ...
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