Search
Coauthors
Kristina Põšnograjeva Institute of Molecular and Cell Biology, University of Tartu, Estonia
1 protocol

Triinu Visnapuu Institute of Molecular and Cell Biology, University of Tartu, Estonia
1 protocol

Katrin Viigand Institute of Molecular and Cell Biology, University of Tartu, Estonia
1 protocol

Karin Ernits Institute of Molecular and Cell Biology, University of Tartu, Estonia
1 protocol

Tiina Alamäe
  • Institute of Molecular and Cell Biology, University of Tartu, Estonia
  • 1 Author merit

Education

Ph.D, University of Tartu, Estonia (defence in Institute of Microbiology, Minsk, Bielorussia), 1986

Current position

Associated Professor , Genetics, Institute of Molecular and Cell Biology, University of Tartu, Estonia

Publications (since 1994)

  1. Mardo, K., Visnapuu, T., Vija, H., Aasamets, A., Viigand, K. and Alamae, T. (2017). A Highly Active Endo-Levanase BT1760 of a Dominant Mammalian Gut Commensal Bacteroides thetaiotaomicron Cleaves Not Only Various Bacterial Levans, but Also Levan of Timothy Grass. PLoS One 12(1): e0169989.
  2. Bondarenko, O. M., Ivask, A., Kahru, A., Vija, H., Titma, T., Visnapuu, M., Joost, U., Pudova, K., Adamberg, S., Visnapuu, T. and Alamae, T. (2016). Bacterial polysaccharide levan as stabilizing, non-toxic and functional coating material for microelement-nanoparticles. Carbohydr Polym 136: 710-720.
  3. Viigand, K., Visnapuu, T., Mardo, K., Aasamets, A. and Alamae, T. (2016). Maltase protein of Ogataea (Hansenula) polymorpha is a counterpart to the resurrected ancestor protein ancMALS of yeast maltases and isomaltases. Yeast 33(8): 415-432.
  4. Adamberg, K., Tomson, K., Talve, T., Pudova, K., Puurand, M., Visnapuu, T., Alamae, T. and Adamberg, S. (2015). Levan Enhances Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota. PLoS One 10(12): e0144042.
  5. Visnapuu, T., Mardo, K. and Alamae, T. (2015). Levansucrases of a Pseudomonas syringae pathovar as catalysts for the synthesis of potentially prebiotic oligo- and polysaccharides. N Biotechnol 32(6): 597-605.
  6. Adamberg, S., Tomson, K., Vija, H., Puurand, M., Kabanova, N., Visnapuu, T., Jogi, E., Alamae, T. and Adamberg, K. (2014). Degradation of Fructans and Production of Propionic Acid by Bacteroides thetaiotaomicron are Enhanced by the Shortage of Amino Acids. Front Nutr 1: 21.
  7. Mardo, K., Visnapuu, T., Gromkova, M., Aasamets, A., Viigand, K., Vija, H. and Alamae, T. (2014). High-throughput assay of levansucrase variants in search of feasible catalysts for the synthesis of fructooligosaccharides and levan. Molecules 19(6): 8434-8455.
  8. Mardo, K., Visnapuu, T., Vija, H., Elmi, T. and Alamae, T. (2014). Mutational analysis of conserved regions harboring catalytic triad residues of the levansucrase protein encoded by the lsc-3 gene (lsc3) of Pseudomonas syringae pv. tomato DC3000. Biotechnol Appl Biochem 61(1): 11-22.
  9. Adamberg, S., Tomson, K., Vija, H., Puurand, M., Kabanova, N., Visnapuu, T., Jogi, E., Alamae, T. and Adamberg, K. (2014). Degradation of Fructans and Production of Propionic Acid by Bacteroides thetaiotaomicron are Enhanced by the Shortage of Amino Acids. Front Nutr 1: 21.
  10. Suppi, S., Michelson, T., Viigand, K. and Alamae, T. (2013). Repression vs. activation of MOX, FMD, MPP1 and MAL1 promoters by sugars in Hansenula polymorpha: the outcome depends on cell's ability to phosphorylate sugar. FEMS Yeast Res 13(2): 219-232.
  11. Pollumaa, L., Alamae, T. and Mae, A. (2012). Quorum sensing and expression of virulence in pectobacteria. Sensors (Basel) 12(3): 3327-3349.
  12. Alamäe, T., Visnapuu, T., Mardo, K., Mäe, A.  Zamfir, A. D. (2012). Levansucrases of Pseudomonas bacteria: novel approaches for protein expression, assay of enzymes, fructooligosaccharides and heterooligofructans. In:  Rauter, A. P. and Lindhorst, T. K. (Eds). Carbohydrate Chemistry. Cambridge, UK: Royal Soc Chemistry, 176–191.
  13. Visnapuu, T., Mardo, K., Mosoarca, C., Zamfir, A. D., Vigants, A. and Alamae, T. (2011). Levansucrases from Pseudomonas syringae pv. tomato and P. chlororaphis subsp. aurantiaca: substrate specificity, polymerizing properties and usage of different acceptors for fructosylation. J Biotechnol 155(3): 338-349.
  14. Visnapuu, T., Mäe, A. and Alamäe, T. (2008). Hansenula polymorpha maltase gene promoter with sigma 70-like elements is feasible for Escherichia coli-based biotechnological applications: expression of three genomic levansucrase genes of Pseudomonas syringae pv. tomato. Process Biochem 43(4): 414-422.
  15. Viigand, K. and Alamae, T. (2007). Further study of the Hansenula polymorpha MAL locus: characterization of the alpha-glucoside permease encoded by the HpMAL2 gene. FEMS Yeast Res 7(7): 1134-1144.
  16. Andresen, L., Koiv, V., Alamae, T. and Mae, A. (2007). The Rcs phosphorelay modulates the expression of plant cell wall degrading enzymes and virulence in Pectobacterium carotovorum ssp. carotovorum. FEMS Microbiol Lett 273(2): 229-238.
  17. Viigand, K., Tammus, K. and Alamae, T. (2005). Clustering of MAL genes in Hansenula polymorpha: cloning of the maltose permease gene and expression from the divergent intergenic region between the maltose permease and maltase genes. FEMS Yeast Res 5(11): 1019-1028.
  18. Karp, H., Jarviste, A., Kriegel, T. M. and Alamae, T. (2003). Cloning and biochemical characterization of hexokinase from the methylotrophic yeast Hansenula polymorpha. Curr Genet 44(5): 268-276.
  19. Alamae, T., Parn, P., Viigand, K. and Karp, H. (2003). Regulation of the Hansenula polymorpha maltase gene promoter in H. polymorpha and Saccharomyces cerevisiae1. FEMS Yeast Res 4(2): 165-173.
  20. Laht, S., Karp, H., Kotka, P., Jarviste, A. and Alamae, T. (2002). Cloning and characterization of glucokinase from a methylotrophic yeast Hansenula polymorpha: different effects on glucose repression in H. polymorpha and Saccharomyces cerevisiae. Gene 296(1-2): 195-203.
  21. Liiv, L., Parn, P. and Alamae, T. (2001). Cloning of maltase gene from a methylotrophic yeast, Hansenula polymorpha. Gene 265(1-2): 77-85.
  22. Alamae, T. and Jarviste, A. (1995). Permeabilization of the methylotrophic yeast pichia-pinus for intracellular enzyme analysis - a quantitative study. J Microbiol Meth 22(2): 193-205.
  23. Alamae, T. and Simisker, J. (1994). Isolation and preliminary characterization of Pichia pinus mutants insensitive to glucose repression. Yeast 10(11): 1459-1466.
1 Protocol published
Thermostability Measurement of an α-Glucosidase Using a Classical Activity-based Assay and a Novel Thermofluor Method
Authors:  Karin Ernits, Katrin Viigand, Triinu Visnapuu, Kristina Põšnograjeva and Tiina Alamäe, date: 06/20/2017, view: 2964, Q&A: 0
α-glucosidases (including maltases and isomaltases) are enzymes which release glucose from a set of α-glucosidic substrates. Their catalytic activity, substrate specificity and thermostability can be assayed using this trait. Thermostability of ...