Diego Wengier 1 protocol

M. Anleu Gil Department of Biology, Stanford University, USA, USA,
1 protocol
Imre Gáspár INSTITUTE OF MOLECULAR BIOTECHNOLOGY, IMBA, Vienna
19 protocols

Shalini Low-Nam Purdue University
12 protocols

Reviewer
Nidhi Sharma
  • HHMI/Stanford University
Research focus
  • Plant science
  • 2 Author merit

Education

Ph.D., The University of Texas at Austin, USA, 2011

Current position

Research Specialist/Lab Manager, Biology, Stanford University/HHMI, United States

Publications

  1. Sharma, N. and Barton, M. K. A novel epistatic mechanism between SHOOTMERISTEMLESS and CLAVATA genes in regulating stem cell population at Shoot Apical Meristem. (Manuscript in preparation).
  2. Magnani, E.,  Longhurst, A., Cabalona, L., Sharma, N., Reinhart, B. J., Liu, T., Talavera-Rauh, F., Huang, T., Hokin, S. A., Kerstetter, R. A. and Barton, M. K. The Abscisic Acid Signaling Pathway and the Ad/Abaxial Developmental Pathway Interact to Regulate Germination and Vegetative Growth in Arabidopsis. (In review).
  3. Sharma, N., Xin, R., Kim, D. H., Sung, S., Lange, T. and Huq, E. (2016). NO FLOWERING IN SHORT DAY (NFL) is a bHLH transcription factor that promotes flowering specifically under short-day conditions in Arabidopsis. Development 143(4): 682-690.
  4. Sharma, N. and Huq, E. (2011). Identification and functional characterization of target genes for NFL. 6:32493.
2 Protocols published
A Quick and Easy Method for Making Competent Escherichia coli Cells for Transformation Using Rubidium Chloride
Authors:  Nidhi Sharma, M. Ximena Anleu Gil and Diego Wengier, date: 11/05/2017, view: 14021, Q&A: 1
This protocol describes a quick and efficient method to make competent E. coli cells for transformation using rubidium chloride. Commercial competent cells are expensive and this protocol provides a cheaper alternative to them.
Leaf Clearing Protocol to Observe Stomata and Other Cells on Leaf Surface
Author:  Nidhi Sharma, date: 09/05/2017, view: 12677, Q&A: 1
In this protocol, leaves are cleared and fixed in an ethanol and acetic acid solution, and mounted in Hoyer’s solution. The cleared leaves are imaged under differential interference contrast (DIC) microscope. This protocol is beneficial for studying ...
2 Protocols reviewed
Detection of Individual RNA in Fixed Cells and Tissues by Chromogenic ISH
Authors:  Meng Jiang, Chen Lin and Rongqin Ke, date: 02/05/2020, view: 1250, Q&A: 0
Visualization of RNA molecules in situ helps to better understand the functions of expressed genes. Currently, most conventional in situ hybridization methods for visualization of individual RNAs are based on fluorescence ...
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Analysis of Metals in Whole Cells, Thylakoids and Photosynthetic Protein Complexes in Synechocystis sp. PCC6803
Authors:  Chiara Gandini, Søren Husted and Sidsel Birkelund Schmidt, date: 06/20/2018, view: 2892, Q&A: 0
Metals are essential in many biological processes, including oxygenic photosynthesis. Here we described a method to measure the metal pool in whole cells and thylakoids, including the bioactive pool in intact photosynthetic protein complexes in the ...
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