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A peer-reviewed protocol journal. No publication fee; no access fee.
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Musa Mhlanga
IDM, University of Cape Town, Cape Town
1 protocol
Sipho Mamputha
Sipho Mamputha
1 protocol
Stoyan Stoychev
1 protocol
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Andrea Puhar
The Laboratory For Molecular Infection Medicine Sweden (MIMS) and Department of Molecular Biology, Umeå University, Sweden
47 protocols
Cristina Suárez
Heidelberg University
2 protocols
Gal Haimovich
Molecular Genetics, Weizmann Institute of Science, Israel
93 protocols
Geoffrey Lau
City University of Hong Kong
18 protocols
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Reviewer
Anca Flavia Savulescu
Research scientist, University of Cape Town
Research focus
Cell biology
Contributions
1
Author merit
Reviewer merit
Editor merit
Education
PhD, Technion, 2011
Lab information
Musa Mhlanga's lab
1 Protocol published
Biochemical Pulldown of mRNAs and Long Noncoding RNAs from Cellular Lysates Coupled with Mass Spectrometry to Identify Protein Binding Partners
Authors:
Anca F. Savulescu
,
Stoyan Stoychev
,
Sipho Mamputha
and
Musa M. Mhlanga
,
date:
06/05/2020,
view:
1101,
Q&A:
0
RNA binding proteins (RBPs) interact with cellular mRNAs, controlling various steps throughout the lifetime of these transcripts, including transcription, cellular transport, subcellular localization, translation and degradation. In addition to ...
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20 Protocols reviewed
Identification of Buffer Conditions for Optimal Thermostability and Solubility of Herpesviral Protein UL37 Using the Thermofluor Assay
Authors:
Andrea L. Koenigsberg
,
Jared D. Pitts
and
Ekaterina E. Heldwein
,
date:
06/20/2020,
view:
594,
Q&A:
0
Structural and biochemical studies of proteins require high amounts of stable, purified proteins. Protein stability often depends on the buffer composition, which includes pH and concentration of salts or other solutes such as glycerol, hence an ...
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A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA
in situ
Hybridization Protocol
Author:
Chiara Sinigaglia
,
date:
09/05/2019,
view:
2276,
Q&A:
0
In situ
hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a ...
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