Musa Mhlanga IDM, University of Cape Town, Cape Town
1 protocol

Sipho Mamputha Sipho Mamputha
1 protocol

Stoyan Stoychev 1 protocol
Andrea Puhar MIMS and Department of Molecular Biology, Umeå University
51 protocols

Cristina Suárez Heidelberg University
3 protocols

Dhruv Patel Oligomerix, Inc.
2 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
95 protocols

Reviewer
Anca Flavia Savulescu
  • Research scientist, University of Cape Town
Research focus
  • Cell biology
  • 1 Author merit

Education

PhD, Technion, 2011

Lab information

Musa Mhlanga's lab

1 Protocol published
Biochemical Pulldown of mRNAs and Long Noncoding RNAs from Cellular Lysates Coupled with Mass Spectrometry to Identify Protein Binding Partners
Authors:  Anca F. Savulescu, Stoyan Stoychev, Sipho Mamputha and Musa M. Mhlanga, date: 06/05/2020, view: 2080, Q&A: 0
RNA binding proteins (RBPs) interact with cellular mRNAs, controlling various steps throughout the lifetime of these transcripts, including transcription, cellular transport, subcellular localization, translation and degradation. In addition to ...
21 Protocols reviewed
Relative Quantification of NaV1.1 Protein in Mouse Brains Using a Meso Scale Discovery-Electrochemiluminescence (MSD-ECL) Method
Authors:  Zhou Han, Anne Christiansen, Meena and Gene Liau, date: 02/05/2021, view: 407, Q&A: 0

Densitometric analysis is often used to quantify NaV1.1 protein on immunoblots, although the sensitivity and dilution linearity of the method are usually poor. Here we present a protocol for quantification of NaV1.1 in mouse brain tissues using a

...
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Identification of Buffer Conditions for Optimal Thermostability and Solubility of Herpesviral Protein UL37 Using the Thermofluor Assay
Authors:  Andrea L. Koenigsberg, Jared D. Pitts and Ekaterina E. Heldwein, date: 06/20/2020, view: 1180, Q&A: 0
Structural and biochemical studies of proteins require high amounts of stable, purified proteins. Protein stability often depends on the buffer composition, which includes pH and concentration of salts or other solutes such as glycerol, hence an ...
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