Manjula Mummadisetti —BIO-PROTOCOL

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Aksiniya Asenova University of California, Davis
14 protocols

Alexandros Alexandratos National and Kapodistrian University of Athens
31 protocols

Amey Redkar Department of Genetics, University of Cordoba
21 protocols

Amit Dey International Centre for Genetic Engineering and Biotechnology
14 protocols

Editor

Manjula Mummadisetti

Post-Doc, Rutgers university

Research focus

Biochemistry
Photosynthesis, Plant Biochemistry, Protein Chemistry, Biofuels, Marine Biology and Environmental Sciences, Biomineralization.

Reviewer merit
Editor merit

Education

PhD, Louisiana State University, 2016

Publications

https://scholar.google.com/citations?user=NT6QpIQAAAAJ&hl=en

4 Protocols reviewed

Biomechanical Characterization of Onion Epidermal Cell Walls

Authors: Daniel M. Durachko, Yong Bum Park, Tian Zhang and Daniel J. Cosgrove, date: 12/20/2017, view: 7187, Q&A: 1

Here we describe two experimental protocols to measure the biomechanical properties of primary (growing) plant cell walls, with a focus on analyzing cell wall epidermal strips of onion scales. The first protocol measures cell wall creep ...

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A Method for Radioactive Labelling of Hebeloma cylindrosporum to Study Plant-fungus Interactions

Authors: Adeline Becquer, Margarita Torres-Aquino, Christine Le Guernevé, Laurie K Amenc, Carlos Trives-Segura, Siobhan Staunton, Hervé Quiquampoix and Claude Plassard, date: 10/20/2017, view: 4646, Q&A: 0

In order to quantify P accumulation and P efflux in the ectomycorrhizal basidiomycete fungus Hebeloma cylindrosporum, we supplied ³²P to mycelia previously grown in vitro in liquid medium. The culture had four main steps ...

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6 Protocols edited

Preparation of HeLa Total Membranes and Assay of Lipid-inhibition of Serine Palmitoyltransferase Activity

Authors: Muthukumar Kannan, Deanna L. Davis, John Suemitsu, Christopher D. Oltorik and Binks Wattenberg, date: 06/20/2020, view: 667, Q&A: 0

Serine palmitoyltranferase (SPT) is a pyridoxal 5′ phosphate (PLP)-dependent enzyme that catalyzes the first and rate-limiting step of de novo synthesis of sphingolipids. SPT activity is homeostatically regulated in response to increased ...

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Negative Ion Mode nanoLC-ESI-MS/MS Analyses of Permethylated Sulfated Glycans

Authors: Shin-Yi Yu, Chu-Wen Cheng and Kay-Hooi Khoo, date: 05/20/2020, view: 894, Q&A: 0

We have developed enabling techniques for sulfoglycomics based on MALDI-MS mapping and MS/MS sequencing of permethylated sulfated glycans. We then extended further the analytical workflow to C18 reverse phase (RP)-nanoLC-nanoESI-MS/MS analyses of ...

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